Optimization of Naked DNA Delivery for Interferon Subtype Immunotherapy in Cytomegalovirus Infection
<p>Type I interferon (IFN) gene therapy modulates the immune response leading to inflammatory heart disease following cytomegalovirus (CMV) infection in a murine model of post-viral myocarditis. Efficacy of different immunisation protocols for the IFN constructs was influenced by the dose of D...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2003-01-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/45/m45.htm |
| _version_ | 1819084837582012416 |
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| author | Bartlett Emmalene J. Cull Vanessa S. Mowe Eva N. Mansfield Josephine P. James Cassandra M. |
| author_facet | Bartlett Emmalene J. Cull Vanessa S. Mowe Eva N. Mansfield Josephine P. James Cassandra M. |
| author_sort | Bartlett Emmalene J. |
| collection | DOAJ |
| description | <p>Type I interferon (IFN) gene therapy modulates the immune response leading to inflammatory heart disease following cytomegalovirus (CMV) infection in a murine model of post-viral myocarditis. Efficacy of different immunisation protocols for the IFN constructs was influenced by the dose of DNA, subtype choice, combination use, pre-medication, and timing of DNA administration. Optimal efficacy was found with bupivacaine treatment prior to DNA inoculation of 200mg <it>IFN</it> DNA 14 days prior to virus challenge. Maximal antiviral and antimyocarditic effects were achieved with this vaccination schedule. Furthermore, inoculation of synergistic IFN subtypes demonstrated enhanced efficacy when delivered either alone or with CMV <it>gB</it> DNA vaccination in the CMV model. Thus naked DNA delivery of IFN provides an avenue of immunotherapy for regulating herpesvirus-induced diseases. |
| first_indexed | 2024-12-21T20:54:48Z |
| format | Article |
| id | doaj.art-2850c8335fee42f2bf1519cd998d8b53 |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-21T20:54:48Z |
| publishDate | 2003-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-2850c8335fee42f2bf1519cd998d8b532022-12-21T18:50:37ZengBMCBiological Procedures Online1480-92222003-01-0151435210.1251/bpo45Optimization of Naked DNA Delivery for Interferon Subtype Immunotherapy in Cytomegalovirus InfectionBartlett Emmalene J.Cull Vanessa S.Mowe Eva N.Mansfield Josephine P.James Cassandra M.<p>Type I interferon (IFN) gene therapy modulates the immune response leading to inflammatory heart disease following cytomegalovirus (CMV) infection in a murine model of post-viral myocarditis. Efficacy of different immunisation protocols for the IFN constructs was influenced by the dose of DNA, subtype choice, combination use, pre-medication, and timing of DNA administration. Optimal efficacy was found with bupivacaine treatment prior to DNA inoculation of 200mg <it>IFN</it> DNA 14 days prior to virus challenge. Maximal antiviral and antimyocarditic effects were achieved with this vaccination schedule. Furthermore, inoculation of synergistic IFN subtypes demonstrated enhanced efficacy when delivered either alone or with CMV <it>gB</it> DNA vaccination in the CMV model. Thus naked DNA delivery of IFN provides an avenue of immunotherapy for regulating herpesvirus-induced diseases.http://www.biologicalprocedures.com/bpo/arts/1/45/m45.htminterferonsgene therapycytomegalovirusDNA |
| spellingShingle | Bartlett Emmalene J. Cull Vanessa S. Mowe Eva N. Mansfield Josephine P. James Cassandra M. Optimization of Naked DNA Delivery for Interferon Subtype Immunotherapy in Cytomegalovirus Infection Biological Procedures Online interferons gene therapy cytomegalovirus DNA |
| title | Optimization of Naked DNA Delivery for Interferon Subtype Immunotherapy in Cytomegalovirus Infection |
| title_full | Optimization of Naked DNA Delivery for Interferon Subtype Immunotherapy in Cytomegalovirus Infection |
| title_fullStr | Optimization of Naked DNA Delivery for Interferon Subtype Immunotherapy in Cytomegalovirus Infection |
| title_full_unstemmed | Optimization of Naked DNA Delivery for Interferon Subtype Immunotherapy in Cytomegalovirus Infection |
| title_short | Optimization of Naked DNA Delivery for Interferon Subtype Immunotherapy in Cytomegalovirus Infection |
| title_sort | optimization of naked dna delivery for interferon subtype immunotherapy in cytomegalovirus infection |
| topic | interferons gene therapy cytomegalovirus DNA |
| url | http://www.biologicalprocedures.com/bpo/arts/1/45/m45.htm |
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