Assembly of anhydrobiotic midge Polypedilum vanderplanki genome using Illumina and PacBio data
A completely new version of assembly of the African anhydrobiotic midge Polypedilum vanderplanki genome derived by deep DNA sequencing of the Pv11 cell line has been discussed. The input data include paired-end and mate-paired reads with various insert sizes supplemented with ultra-long reads of Pa...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Kazan Federal University
2018-06-01
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| Series: | Учёные записки Казанского университета: Серия Естественные науки |
| Subjects: | |
| Online Access: | https://kpfu.ru/assembly-of-anhydrobiotic-midge-polypedilum-342807.html |
| _version_ | 1826908537589071872 |
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| author | O.S. Kozlova Z.I. Abramova |
| author_facet | O.S. Kozlova Z.I. Abramova |
| author_sort | O.S. Kozlova |
| collection | DOAJ |
| description | A completely new version of assembly of the African anhydrobiotic midge Polypedilum vanderplanki genome derived by deep DNA sequencing of the Pv11 cell line has been discussed. The input data include paired-end and mate-paired reads with various insert sizes supplemented with ultra-long reads of Pacific Biosciences platform sequencing. We have shown that the resulting set of scaffolds has higher continuity and completeness metrics and, besides, can provide more correct predictions of coding sequences as compared to the previous assembly version, which has been demonstrated based on heat-shock proteins HSP20 and HSP70. |
| first_indexed | 2024-03-13T07:03:05Z |
| format | Article |
| id | doaj.art-285267a9ccb14c668b90a7746004995c |
| institution | Directory Open Access Journal |
| issn | 2542-064X 2500-218X |
| language | English |
| last_indexed | 2025-02-17T09:26:21Z |
| publishDate | 2018-06-01 |
| publisher | Kazan Federal University |
| record_format | Article |
| series | Учёные записки Казанского университета: Серия Естественные науки |
| spelling | doaj.art-285267a9ccb14c668b90a7746004995c2025-01-02T11:41:00ZengKazan Federal UniversityУчёные записки Казанского университета: Серия Естественные науки2542-064X2500-218X2018-06-011602214226Assembly of anhydrobiotic midge Polypedilum vanderplanki genome using Illumina and PacBio dataO.S. Kozlova0Z.I. Abramova1Kazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaA completely new version of assembly of the African anhydrobiotic midge Polypedilum vanderplanki genome derived by deep DNA sequencing of the Pv11 cell line has been discussed. The input data include paired-end and mate-paired reads with various insert sizes supplemented with ultra-long reads of Pacific Biosciences platform sequencing. We have shown that the resulting set of scaffolds has higher continuity and completeness metrics and, besides, can provide more correct predictions of coding sequences as compared to the previous assembly version, which has been demonstrated based on heat-shock proteins HSP20 and HSP70.https://kpfu.ru/assembly-of-anhydrobiotic-midge-polypedilum-342807.htmlpolypedilum vanderplankianhydrobiosisdna sequencinggenome assembly |
| spellingShingle | O.S. Kozlova Z.I. Abramova Assembly of anhydrobiotic midge Polypedilum vanderplanki genome using Illumina and PacBio data Учёные записки Казанского университета: Серия Естественные науки polypedilum vanderplanki anhydrobiosis dna sequencing genome assembly |
| title | Assembly of anhydrobiotic midge Polypedilum vanderplanki genome using Illumina and PacBio data |
| title_full | Assembly of anhydrobiotic midge Polypedilum vanderplanki genome using Illumina and PacBio data |
| title_fullStr | Assembly of anhydrobiotic midge Polypedilum vanderplanki genome using Illumina and PacBio data |
| title_full_unstemmed | Assembly of anhydrobiotic midge Polypedilum vanderplanki genome using Illumina and PacBio data |
| title_short | Assembly of anhydrobiotic midge Polypedilum vanderplanki genome using Illumina and PacBio data |
| title_sort | assembly of anhydrobiotic midge polypedilum vanderplanki genome using illumina and pacbio data |
| topic | polypedilum vanderplanki anhydrobiosis dna sequencing genome assembly |
| url | https://kpfu.ru/assembly-of-anhydrobiotic-midge-polypedilum-342807.html |
| work_keys_str_mv | AT oskozlova assemblyofanhydrobioticmidgepolypedilumvanderplankigenomeusingilluminaandpacbiodata AT ziabramova assemblyofanhydrobioticmidgepolypedilumvanderplankigenomeusingilluminaandpacbiodata |