Detection of Phage-Induced Mobile Genetic Elements in Strains of <i>Vibrio cholerae</i> O1 Biovar El Tor
The acquisition of new mobile genetic elements contributes to the genetic diversity of Vibrio cholerae strains. An important role in this process belongs to the genetic material obtained from phages. The aim of this work was to identify phage-induced PLE islands in strains of V. cholerae O1 serogrou...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2023-07-01
|
| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/1828 |
| _version_ | 1797220718795030528 |
|---|---|
| author | S. P. Zadnova N. A. Plekhanov A. Yu. Spirina I. G. Shvidenko V. N. Savel’ev |
| author_facet | S. P. Zadnova N. A. Plekhanov A. Yu. Spirina I. G. Shvidenko V. N. Savel’ev |
| author_sort | S. P. Zadnova |
| collection | DOAJ |
| description | The acquisition of new mobile genetic elements contributes to the genetic diversity of Vibrio cholerae strains. An important role in this process belongs to the genetic material obtained from phages. The aim of this work was to identify phage-induced PLE islands in strains of V. cholerae O1 serogroup and to determine the resistance of isolates with and without those mobile genetic elements to the lytic activity of the diagnostic cholera El Tor bacteriophage. Materials and methods. Whole genomes nucleotide sequences of toxigenic and non-toxigenic V. cholerae O1 strains presented in the NCBI GenBank were used for the work. Bioinformatic analysis was performed using the BLAST algorithm, MEGA X (or BioEdit v. 7.0.9.0). The test with phages was carried out according A. Gratia technique. Results and discussion. The analysis of 39 toxigenic strains imported to the territory of the Russian Federation and neighboring countries has revealed one strain of V. cholerae O1 of the classical biovar containing the PLE5 island, and 13 strains of V. cholerae O1 of the El Tor biovar containing the PLE4 island. PLE islands have not been found in non-toxigenic strains. It is shown that strains with PLE4 belong to V. cholerae O1 genovariants of the El Tor biovar and have the ctxB1 gene allele. Isolates with this mobile element caused sporadic cases of the disease in 1994–1999, as well as cholera outbreaks in the Russian Federation (in 1993–1994, in 1998 – Dagestan, and 1993 – Tatarstan) and Ukraine (1994–1995). It has been suggested that, perhaps, the presence of the PLE4 island makes a certain contribution to the resistance of V. cholerae O1 strains of the El Tor biovar to the diagnostic cholera El Tor phage (55.6 % of phage-resistant isolates were detected), but there are other mechanisms that have not yet been identified. Thus, the data on the presence of new mobile genetic elements in the genome of earlier imported toxigenic strains of V. cholerae O1, biovar El Tor have been obtained, which expands information about their genetic organization. |
| first_indexed | 2024-03-08T13:40:57Z |
| format | Article |
| id | doaj.art-285486b9de604ab6925cbc59aa2c6728 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:54:00Z |
| publishDate | 2023-07-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-285486b9de604ab6925cbc59aa2c67282024-04-05T16:47:18ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2023-07-010211211910.21055/0370-1069-2023-2-112-1191463Detection of Phage-Induced Mobile Genetic Elements in Strains of <i>Vibrio cholerae</i> O1 Biovar El TorS. P. Zadnova0N. A. Plekhanov1A. Yu. Spirina2I. G. Shvidenko3V. N. Savel’ev4Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Stavropol Research Anti-Plague InstituteThe acquisition of new mobile genetic elements contributes to the genetic diversity of Vibrio cholerae strains. An important role in this process belongs to the genetic material obtained from phages. The aim of this work was to identify phage-induced PLE islands in strains of V. cholerae O1 serogroup and to determine the resistance of isolates with and without those mobile genetic elements to the lytic activity of the diagnostic cholera El Tor bacteriophage. Materials and methods. Whole genomes nucleotide sequences of toxigenic and non-toxigenic V. cholerae O1 strains presented in the NCBI GenBank were used for the work. Bioinformatic analysis was performed using the BLAST algorithm, MEGA X (or BioEdit v. 7.0.9.0). The test with phages was carried out according A. Gratia technique. Results and discussion. The analysis of 39 toxigenic strains imported to the territory of the Russian Federation and neighboring countries has revealed one strain of V. cholerae O1 of the classical biovar containing the PLE5 island, and 13 strains of V. cholerae O1 of the El Tor biovar containing the PLE4 island. PLE islands have not been found in non-toxigenic strains. It is shown that strains with PLE4 belong to V. cholerae O1 genovariants of the El Tor biovar and have the ctxB1 gene allele. Isolates with this mobile element caused sporadic cases of the disease in 1994–1999, as well as cholera outbreaks in the Russian Federation (in 1993–1994, in 1998 – Dagestan, and 1993 – Tatarstan) and Ukraine (1994–1995). It has been suggested that, perhaps, the presence of the PLE4 island makes a certain contribution to the resistance of V. cholerae O1 strains of the El Tor biovar to the diagnostic cholera El Tor phage (55.6 % of phage-resistant isolates were detected), but there are other mechanisms that have not yet been identified. Thus, the data on the presence of new mobile genetic elements in the genome of earlier imported toxigenic strains of V. cholerae O1, biovar El Tor have been obtained, which expands information about their genetic organization.https://journal.microbe.ru/jour/article/view/1828vibrio choleraeple islandslysis by diagnostic cholera el tor phage |
| spellingShingle | S. P. Zadnova N. A. Plekhanov A. Yu. Spirina I. G. Shvidenko V. N. Savel’ev Detection of Phage-Induced Mobile Genetic Elements in Strains of <i>Vibrio cholerae</i> O1 Biovar El Tor Проблемы особо опасных инфекций vibrio cholerae ple islands lysis by diagnostic cholera el tor phage |
| title | Detection of Phage-Induced Mobile Genetic Elements in Strains of <i>Vibrio cholerae</i> O1 Biovar El Tor |
| title_full | Detection of Phage-Induced Mobile Genetic Elements in Strains of <i>Vibrio cholerae</i> O1 Biovar El Tor |
| title_fullStr | Detection of Phage-Induced Mobile Genetic Elements in Strains of <i>Vibrio cholerae</i> O1 Biovar El Tor |
| title_full_unstemmed | Detection of Phage-Induced Mobile Genetic Elements in Strains of <i>Vibrio cholerae</i> O1 Biovar El Tor |
| title_short | Detection of Phage-Induced Mobile Genetic Elements in Strains of <i>Vibrio cholerae</i> O1 Biovar El Tor |
| title_sort | detection of phage induced mobile genetic elements in strains of i vibrio cholerae i o1 biovar el tor |
| topic | vibrio cholerae ple islands lysis by diagnostic cholera el tor phage |
| url | https://journal.microbe.ru/jour/article/view/1828 |
| work_keys_str_mv | AT spzadnova detectionofphageinducedmobilegeneticelementsinstrainsofivibriocholeraeio1biovareltor AT naplekhanov detectionofphageinducedmobilegeneticelementsinstrainsofivibriocholeraeio1biovareltor AT ayuspirina detectionofphageinducedmobilegeneticelementsinstrainsofivibriocholeraeio1biovareltor AT igshvidenko detectionofphageinducedmobilegeneticelementsinstrainsofivibriocholeraeio1biovareltor AT vnsavelev detectionofphageinducedmobilegeneticelementsinstrainsofivibriocholeraeio1biovareltor |