Fabrication of polycaprolactone/calcium phosphates hybrid scaffolds impregnated with plant extracts using 3D printing for potential bone regeneration
The increase in critical bone diseases and defects in the world's population increases the need for bone substitutes to restore form and function. Organic and inorganic scaffolds with antibacterial properties could provide advantages for bone regeneration. In this study, we obtained scaffolds o...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023003833 |
| _version_ | 1828000835914694656 |
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| author | Claudia Garcia Yeison Orozco Alejandra Betancur Ana Isabel Moreno Katherine Fuentes Alex Lopera Oscar Suarez Tatiana Lobo Alexander Ossa Alejandro Peláez-Vargas Carlos Paucar |
| author_facet | Claudia Garcia Yeison Orozco Alejandra Betancur Ana Isabel Moreno Katherine Fuentes Alex Lopera Oscar Suarez Tatiana Lobo Alexander Ossa Alejandro Peláez-Vargas Carlos Paucar |
| author_sort | Claudia Garcia |
| collection | DOAJ |
| description | The increase in critical bone diseases and defects in the world's population increases the need for bone substitutes to restore form and function. Organic and inorganic scaffolds with antibacterial properties could provide advantages for bone regeneration. In this study, we obtained scaffolds of polycaprolactone (PCL) charged with calcium phosphates nanoparticles and impregnated with extracts of Colombian plants as an alternative for potential bone regeneration. Calcium phosphate nanoparticles were obtained via auto-combustion synthesis. The nanoparticles were incorporated into the PCL with a chemical dissolution-disperse process. The composite obtained was used to produce a filament to print Triply Periodic Minimal Surface (TPMS) based scaffolds. Such geometry facilitates cellular growth thanks to its interconnected porosity. The scaffolds were impregnated with extracts of Justicia cf colorifera (Acanthaceae), and Billia rosea (Sapindaceae) due to their ancestral medical applications. A physical and biological characterization was conducted. The process to print scaffolds with an enhanced geometry to facilitate the flux of biological fluids was successful. The scaffolds loaded with B. rosea showed strong antibacterial behavior, suggesting the presence of reported terpenoids with antibacterial properties. The approach used in this study evidenced promising prospects for bone defect repair. |
| first_indexed | 2024-04-10T06:20:20Z |
| format | Article |
| id | doaj.art-2855805d67264d6a97a04b0c0093cdc3 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-10T06:20:20Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-2855805d67264d6a97a04b0c0093cdc32023-03-02T05:00:21ZengElsevierHeliyon2405-84402023-02-0192e13176Fabrication of polycaprolactone/calcium phosphates hybrid scaffolds impregnated with plant extracts using 3D printing for potential bone regenerationClaudia Garcia0Yeison Orozco1Alejandra Betancur2Ana Isabel Moreno3Katherine Fuentes4Alex Lopera5Oscar Suarez6Tatiana Lobo7Alexander Ossa8Alejandro Peláez-Vargas9Carlos Paucar10Universidad Nacional de Colombia sede Medellín, Physics school, Grupo de Materiales Cerámicos y Vítreos, Colombia; Corresponding author. Universidad Nacional de Colombia sede Medellín, Carrera 65 # 59A-100, Medellín, Colombia.Universidad Nacional de Colombia sede Medellín, Grupo de Materiales Cerámicos y Vítreos, ColombiaUniversidad Nacional de Colombia sede Medellín, Grupo de Materiales Cerámicos y Vítreos, ColombiaUniversidad Nacional de Colombia sede Medellín, Grupo de Materiales Cerámicos y Vítreos, ColombiaUniversidad Nacional de Colombia sede Medellín, ColombiaGrupo de Nanoestructuras y Física Aplicada (NANOUPAR), Dirección Académica, Universidad Nacional de Colombia, Sede de La Paz, Km 9 vía Valledupar La Paz, La Paz 202010, ColombiaUniversidad Nacional de Colombia sede Orinoquia, ColombiaUniversidad Nacional de Colombia sede Medellín, Chemistry school, ColombiaSchool of Applied Sciences and Engineering, Universidad Eafit, Medellín, ColombiaUniversidad Cooperativa de Colombia, Medellín, ColombiaUniversidad Nacional de Colombia sede Medellín, Chemistry school, Grupo de Materiales Cerámicos y Vítreos, ColombiaThe increase in critical bone diseases and defects in the world's population increases the need for bone substitutes to restore form and function. Organic and inorganic scaffolds with antibacterial properties could provide advantages for bone regeneration. In this study, we obtained scaffolds of polycaprolactone (PCL) charged with calcium phosphates nanoparticles and impregnated with extracts of Colombian plants as an alternative for potential bone regeneration. Calcium phosphate nanoparticles were obtained via auto-combustion synthesis. The nanoparticles were incorporated into the PCL with a chemical dissolution-disperse process. The composite obtained was used to produce a filament to print Triply Periodic Minimal Surface (TPMS) based scaffolds. Such geometry facilitates cellular growth thanks to its interconnected porosity. The scaffolds were impregnated with extracts of Justicia cf colorifera (Acanthaceae), and Billia rosea (Sapindaceae) due to their ancestral medical applications. A physical and biological characterization was conducted. The process to print scaffolds with an enhanced geometry to facilitate the flux of biological fluids was successful. The scaffolds loaded with B. rosea showed strong antibacterial behavior, suggesting the presence of reported terpenoids with antibacterial properties. The approach used in this study evidenced promising prospects for bone defect repair.http://www.sciencedirect.com/science/article/pii/S2405844023003833Scaffold3-D printingNatural extractsImpregnation methodPolycaprolactoneCalcium phosphate |
| spellingShingle | Claudia Garcia Yeison Orozco Alejandra Betancur Ana Isabel Moreno Katherine Fuentes Alex Lopera Oscar Suarez Tatiana Lobo Alexander Ossa Alejandro Peláez-Vargas Carlos Paucar Fabrication of polycaprolactone/calcium phosphates hybrid scaffolds impregnated with plant extracts using 3D printing for potential bone regeneration Heliyon Scaffold 3-D printing Natural extracts Impregnation method Polycaprolactone Calcium phosphate |
| title | Fabrication of polycaprolactone/calcium phosphates hybrid scaffolds impregnated with plant extracts using 3D printing for potential bone regeneration |
| title_full | Fabrication of polycaprolactone/calcium phosphates hybrid scaffolds impregnated with plant extracts using 3D printing for potential bone regeneration |
| title_fullStr | Fabrication of polycaprolactone/calcium phosphates hybrid scaffolds impregnated with plant extracts using 3D printing for potential bone regeneration |
| title_full_unstemmed | Fabrication of polycaprolactone/calcium phosphates hybrid scaffolds impregnated with plant extracts using 3D printing for potential bone regeneration |
| title_short | Fabrication of polycaprolactone/calcium phosphates hybrid scaffolds impregnated with plant extracts using 3D printing for potential bone regeneration |
| title_sort | fabrication of polycaprolactone calcium phosphates hybrid scaffolds impregnated with plant extracts using 3d printing for potential bone regeneration |
| topic | Scaffold 3-D printing Natural extracts Impregnation method Polycaprolactone Calcium phosphate |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023003833 |
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