Identification and Characterization of Resistance Loci to Wheat Leaf Rust and Stripe Rust in Afghan Landrace “KU3067”
Leaf rust and stripe rust are important wheat diseases worldwide causing significant losses where susceptible varieties are grown. Resistant cultivars offer long-term control and reduce the use of hazardous chemicals, which can be detrimental to both human health and the environment. Land races have...
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.894528/full |
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| author | Peipei Zhang Caixia Lan Ravi P. Singh Julio Huerta-Espino Zaifeng Li Evans Lagudah Sridhar Bhavani |
| author_facet | Peipei Zhang Caixia Lan Ravi P. Singh Julio Huerta-Espino Zaifeng Li Evans Lagudah Sridhar Bhavani |
| author_sort | Peipei Zhang |
| collection | DOAJ |
| description | Leaf rust and stripe rust are important wheat diseases worldwide causing significant losses where susceptible varieties are grown. Resistant cultivars offer long-term control and reduce the use of hazardous chemicals, which can be detrimental to both human health and the environment. Land races have been a valuable resource for mining new genes for various abiotic and biotic stresses including wheat rusts. Afghan wheat landrace “KU3067” displayed high seedling infection type (IT) for leaf rust and low IT for stripe rust; however, it displayed high levels of field resistance for both rusts when tested for multiple seasons against the Mexican rust isolates. This study focused on identifying loci-conferring seedling resistance to stripe rust, and also loci-conferring adult plant resistance (APR) against the Mexican races of leaf rust and stripe rust. A backcrossed inbred line (BIL) population advanced to the BC1F5 generation derived from the cross of KU3067 and Apav (triple rust susceptible line) was used for both, inheritance and QTL mapping studies. The population and parents were genotyped with Diversity Arrays Technology-genotyping-by-sequencing (DArT-Seq) and phenotyped for leaf rust and stripe rust response at both seedling and adult plant stages during multiple seasons in Mexico with relevant pathotypes. Mapping results identified an all-stage resistance gene for stripe rust, temporarily designated as YrKU, on chromosome 7BL. In total, six QTL-conferring APR to leaf rust on 1AS, 2AL, 4DL, 6BL, 7AL, and 7BL, and four QTL for stripe rust resistance on 1BS, 2AL, 4DL, and 7BL were detected in the analyses. Among these, pleiotropic gene Lr67/Yr46 on 4DL with a significantly large effect is the first report in an Afghan landrace-conferring resistance to both leaf and stripe rusts. QLr.cim-7BL/YrKU showed pleiotropic resistance to both rusts and explained 7.5–17.2 and 12.6–19.3% of the phenotypic variance for leaf and stripe rusts, respectively. QYr.cim-1BS and QYr.cim-2AL detected in all stripe environments with phenotypic variance explained (PVE) 12.9–20.5 and 5.4–12.5%, and QLr.cim-6BL are likely to be new. These QTL and their closely linked markers will be useful for fine mapping and marker-assisted selection (MAS) in breeding for durable resistance to multiple rust diseases. |
| first_indexed | 2024-04-12T12:19:38Z |
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| id | doaj.art-285707504c1d4c808d9000fab72b6edf |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-04-12T12:19:38Z |
| publishDate | 2022-06-01 |
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| spelling | doaj.art-285707504c1d4c808d9000fab72b6edf2022-12-22T03:33:20ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-06-011310.3389/fpls.2022.894528894528Identification and Characterization of Resistance Loci to Wheat Leaf Rust and Stripe Rust in Afghan Landrace “KU3067”Peipei Zhang0Caixia Lan1Ravi P. Singh2Julio Huerta-Espino3Zaifeng Li4Evans Lagudah5Sridhar Bhavani6State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding, ChinaHubei Hongshan Laboratory, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, ChinaInternational Maize and Wheat Improvement Center (CIMMYT), Texcoco, MexicoCampo Experimental Valle de México the National Institute of Forestry, Agricultural and Livestock Research (INIFAP), Texcoco, MexicoState Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding, ChinaCommonwealth Scientific and Industrial Research Organisation (CSIRO) Plant Industry, Canberra, ACT, AustraliaInternational Maize and Wheat Improvement Center (CIMMYT), Texcoco, MexicoLeaf rust and stripe rust are important wheat diseases worldwide causing significant losses where susceptible varieties are grown. Resistant cultivars offer long-term control and reduce the use of hazardous chemicals, which can be detrimental to both human health and the environment. Land races have been a valuable resource for mining new genes for various abiotic and biotic stresses including wheat rusts. Afghan wheat landrace “KU3067” displayed high seedling infection type (IT) for leaf rust and low IT for stripe rust; however, it displayed high levels of field resistance for both rusts when tested for multiple seasons against the Mexican rust isolates. This study focused on identifying loci-conferring seedling resistance to stripe rust, and also loci-conferring adult plant resistance (APR) against the Mexican races of leaf rust and stripe rust. A backcrossed inbred line (BIL) population advanced to the BC1F5 generation derived from the cross of KU3067 and Apav (triple rust susceptible line) was used for both, inheritance and QTL mapping studies. The population and parents were genotyped with Diversity Arrays Technology-genotyping-by-sequencing (DArT-Seq) and phenotyped for leaf rust and stripe rust response at both seedling and adult plant stages during multiple seasons in Mexico with relevant pathotypes. Mapping results identified an all-stage resistance gene for stripe rust, temporarily designated as YrKU, on chromosome 7BL. In total, six QTL-conferring APR to leaf rust on 1AS, 2AL, 4DL, 6BL, 7AL, and 7BL, and four QTL for stripe rust resistance on 1BS, 2AL, 4DL, and 7BL were detected in the analyses. Among these, pleiotropic gene Lr67/Yr46 on 4DL with a significantly large effect is the first report in an Afghan landrace-conferring resistance to both leaf and stripe rusts. QLr.cim-7BL/YrKU showed pleiotropic resistance to both rusts and explained 7.5–17.2 and 12.6–19.3% of the phenotypic variance for leaf and stripe rusts, respectively. QYr.cim-1BS and QYr.cim-2AL detected in all stripe environments with phenotypic variance explained (PVE) 12.9–20.5 and 5.4–12.5%, and QLr.cim-6BL are likely to be new. These QTL and their closely linked markers will be useful for fine mapping and marker-assisted selection (MAS) in breeding for durable resistance to multiple rust diseases.https://www.frontiersin.org/articles/10.3389/fpls.2022.894528/fullgenetic analysismolecular mappingwheat rustsAPR geneslandraces |
| spellingShingle | Peipei Zhang Caixia Lan Ravi P. Singh Julio Huerta-Espino Zaifeng Li Evans Lagudah Sridhar Bhavani Identification and Characterization of Resistance Loci to Wheat Leaf Rust and Stripe Rust in Afghan Landrace “KU3067” Frontiers in Plant Science genetic analysis molecular mapping wheat rusts APR genes landraces |
| title | Identification and Characterization of Resistance Loci to Wheat Leaf Rust and Stripe Rust in Afghan Landrace “KU3067” |
| title_full | Identification and Characterization of Resistance Loci to Wheat Leaf Rust and Stripe Rust in Afghan Landrace “KU3067” |
| title_fullStr | Identification and Characterization of Resistance Loci to Wheat Leaf Rust and Stripe Rust in Afghan Landrace “KU3067” |
| title_full_unstemmed | Identification and Characterization of Resistance Loci to Wheat Leaf Rust and Stripe Rust in Afghan Landrace “KU3067” |
| title_short | Identification and Characterization of Resistance Loci to Wheat Leaf Rust and Stripe Rust in Afghan Landrace “KU3067” |
| title_sort | identification and characterization of resistance loci to wheat leaf rust and stripe rust in afghan landrace ku3067 |
| topic | genetic analysis molecular mapping wheat rusts APR genes landraces |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.894528/full |
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