Tissue-based diagnosis of systemic amyloidosis: Experience of the informal diagnostic center at Uppsala University Hospital
Diagnosis of systemic amyloidosis is a clinical challenge and usually relies on a tissue biopsy. We have developed diagnostic methods based on the presence of amyloid deposits in abdominal subcutaneous fat tissue. This tissue is also used to determine the biochemical type of amyloidosis, performed b...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Upsala Medical Society
2022-09-01
|
| Series: | Upsala Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://ujms.net/index.php/ujms/article/view/8913/14885 |
| _version_ | 1797705239120314368 |
|---|---|
| author | Justina Damjanovic Vesterlund Elisabet Ihse Ulrika Thelander Alice Zancanaro Gunilla T. Westermark Per Westermark |
| author_facet | Justina Damjanovic Vesterlund Elisabet Ihse Ulrika Thelander Alice Zancanaro Gunilla T. Westermark Per Westermark |
| author_sort | Justina Damjanovic Vesterlund |
| collection | DOAJ |
| description | Diagnosis of systemic amyloidosis is a clinical challenge and usually relies on a tissue biopsy. We have developed diagnostic methods based on the presence of amyloid deposits in abdominal subcutaneous fat tissue. This tissue is also used to determine the biochemical type of amyloidosis, performed by western blot and immunohistochemical analyses with the aid of in-house developed rabbit antisera and mouse monoclonal antibodies. Mass spectrometric methods are under development for selected cases. The diagnostic outcome for 2018-2020 was studied. During this period, we obtained 1,562 biopsies, of which 1,397 were unfixed subcutaneous fat tissue with varying degrees of suspicion of systemic amyloidosis. Of these, 440 contained amyloid deposits. The biochemical nature of the amyloid was determined by western blot analysis in 319 specimens and by immunohistochemistry in further 51 cases. |
| first_indexed | 2024-03-12T05:33:12Z |
| format | Article |
| id | doaj.art-285a18c9f08e469391cc5aab4d296091 |
| institution | Directory Open Access Journal |
| issn | 0300-9734 2000-1967 |
| language | English |
| last_indexed | 2024-03-12T05:33:12Z |
| publishDate | 2022-09-01 |
| publisher | Upsala Medical Society |
| record_format | Article |
| series | Upsala Journal of Medical Sciences |
| spelling | doaj.art-285a18c9f08e469391cc5aab4d2960912023-09-03T06:33:43ZengUpsala Medical SocietyUpsala Journal of Medical Sciences0300-97342000-19672022-09-011271810.48101/ujms.v127.89138913Tissue-based diagnosis of systemic amyloidosis: Experience of the informal diagnostic center at Uppsala University HospitalJustina Damjanovic Vesterlund0Elisabet Ihse1Ulrika Thelander2Alice Zancanaro3Gunilla T. Westermark4Per Westermark5Clinical Pathology, Uppsala University HospitalClinical Pathology, Uppsala University HospitalClinical Pathology, Uppsala University HospitalDepartment of Genetics, Immunology and PathologyDepartment of Medical Cell Biology, Uppsala University, Uppsala, SwedenClinical Pathology, Uppsala University HospitalDiagnosis of systemic amyloidosis is a clinical challenge and usually relies on a tissue biopsy. We have developed diagnostic methods based on the presence of amyloid deposits in abdominal subcutaneous fat tissue. This tissue is also used to determine the biochemical type of amyloidosis, performed by western blot and immunohistochemical analyses with the aid of in-house developed rabbit antisera and mouse monoclonal antibodies. Mass spectrometric methods are under development for selected cases. The diagnostic outcome for 2018-2020 was studied. During this period, we obtained 1,562 biopsies, of which 1,397 were unfixed subcutaneous fat tissue with varying degrees of suspicion of systemic amyloidosis. Of these, 440 contained amyloid deposits. The biochemical nature of the amyloid was determined by western blot analysis in 319 specimens and by immunohistochemistry in further 51 cases.https://ujms.net/index.php/ujms/article/view/8913/14885amyloidosissystemicmonoclonal antibodiessubcutaneous fat tissuebiopsy |
| spellingShingle | Justina Damjanovic Vesterlund Elisabet Ihse Ulrika Thelander Alice Zancanaro Gunilla T. Westermark Per Westermark Tissue-based diagnosis of systemic amyloidosis: Experience of the informal diagnostic center at Uppsala University Hospital Upsala Journal of Medical Sciences amyloidosis systemic monoclonal antibodies subcutaneous fat tissue biopsy |
| title | Tissue-based diagnosis of systemic amyloidosis: Experience of the informal diagnostic center at Uppsala University Hospital |
| title_full | Tissue-based diagnosis of systemic amyloidosis: Experience of the informal diagnostic center at Uppsala University Hospital |
| title_fullStr | Tissue-based diagnosis of systemic amyloidosis: Experience of the informal diagnostic center at Uppsala University Hospital |
| title_full_unstemmed | Tissue-based diagnosis of systemic amyloidosis: Experience of the informal diagnostic center at Uppsala University Hospital |
| title_short | Tissue-based diagnosis of systemic amyloidosis: Experience of the informal diagnostic center at Uppsala University Hospital |
| title_sort | tissue based diagnosis of systemic amyloidosis experience of the informal diagnostic center at uppsala university hospital |
| topic | amyloidosis systemic monoclonal antibodies subcutaneous fat tissue biopsy |
| url | https://ujms.net/index.php/ujms/article/view/8913/14885 |
| work_keys_str_mv | AT justinadamjanovicvesterlund tissuebaseddiagnosisofsystemicamyloidosisexperienceoftheinformaldiagnosticcenteratuppsalauniversityhospital AT elisabetihse tissuebaseddiagnosisofsystemicamyloidosisexperienceoftheinformaldiagnosticcenteratuppsalauniversityhospital AT ulrikathelander tissuebaseddiagnosisofsystemicamyloidosisexperienceoftheinformaldiagnosticcenteratuppsalauniversityhospital AT alicezancanaro tissuebaseddiagnosisofsystemicamyloidosisexperienceoftheinformaldiagnosticcenteratuppsalauniversityhospital AT gunillatwestermark tissuebaseddiagnosisofsystemicamyloidosisexperienceoftheinformaldiagnosticcenteratuppsalauniversityhospital AT perwestermark tissuebaseddiagnosisofsystemicamyloidosisexperienceoftheinformaldiagnosticcenteratuppsalauniversityhospital |