Comprehensive Analysis of Soluble Mediator Profiles in Congenital CMV Infection Using an MCMV Model
Congenital human cytomegalovirus (HCMV) infection may cause life-threatening disease and permanent damage to the central nervous system. The mouse model of CMV infection is most commonly used to study mechanisms of infection and pathogenesis. While essential to limit mouse CMV (MCMV) replication, th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/16/2/208 |
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| author | Dubravka Karner Daria Kvestak Berislav Lisnic Maja Cokaric Brdovcak Vanda Juranic Lisnic Paola Kucan Brlic Milena Hasan Tihana Lenac Rovis |
| author_facet | Dubravka Karner Daria Kvestak Berislav Lisnic Maja Cokaric Brdovcak Vanda Juranic Lisnic Paola Kucan Brlic Milena Hasan Tihana Lenac Rovis |
| author_sort | Dubravka Karner |
| collection | DOAJ |
| description | Congenital human cytomegalovirus (HCMV) infection may cause life-threatening disease and permanent damage to the central nervous system. The mouse model of CMV infection is most commonly used to study mechanisms of infection and pathogenesis. While essential to limit mouse CMV (MCMV) replication, the inflammatory responses, particularly IFNγ and TNFα, cause neurodevelopmental abnormalities. Other soluble mediators of the immune response in most tissues remain largely unexplored. To address this gap, we quantified 48 soluble mediators of the immune response, including 32 cytokines, 10 chemokines, 3 growth factors/regulators, and 3 soluble receptors in the spleen, liver, lungs, and brain at 9 and 14 days postinfection (dpi). Our analysis found 25 induced molecules in the brain at 9 dpi, with an additional 8 showing statistically elevated responses at 14 dpi. Specifically, all analyzed CCL group cytokines (CCL2, CCL3, CCL4, CCL5, CCL7, and CCL11) were upregulated at 14 dpi in the brain. Furthermore, data revealed differentially regulated analytes across tissues, such as CCL11, CXCL5, and IL-10 in the brain, IL-33/IL-33R in the liver, and VEGF-a and IL-5 in the lungs. Overall, this study provides an overview of the immune dynamics of soluble mediators in congenital CMV. |
| first_indexed | 2024-03-07T22:10:50Z |
| format | Article |
| id | doaj.art-285d5ae4b0af41e8bc34919de725d020 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-07T22:10:50Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-285d5ae4b0af41e8bc34919de725d0202024-02-23T15:37:29ZengMDPI AGViruses1999-49152024-01-0116220810.3390/v16020208Comprehensive Analysis of Soluble Mediator Profiles in Congenital CMV Infection Using an MCMV ModelDubravka Karner0Daria Kvestak1Berislav Lisnic2Maja Cokaric Brdovcak3Vanda Juranic Lisnic4Paola Kucan Brlic5Milena Hasan6Tihana Lenac Rovis7Center for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaCytometry and Biomarkers Unit of Technology and Service (CB TechS), Institut Pasteur, Université Paris Cité, 75015 Paris, FranceCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaCongenital human cytomegalovirus (HCMV) infection may cause life-threatening disease and permanent damage to the central nervous system. The mouse model of CMV infection is most commonly used to study mechanisms of infection and pathogenesis. While essential to limit mouse CMV (MCMV) replication, the inflammatory responses, particularly IFNγ and TNFα, cause neurodevelopmental abnormalities. Other soluble mediators of the immune response in most tissues remain largely unexplored. To address this gap, we quantified 48 soluble mediators of the immune response, including 32 cytokines, 10 chemokines, 3 growth factors/regulators, and 3 soluble receptors in the spleen, liver, lungs, and brain at 9 and 14 days postinfection (dpi). Our analysis found 25 induced molecules in the brain at 9 dpi, with an additional 8 showing statistically elevated responses at 14 dpi. Specifically, all analyzed CCL group cytokines (CCL2, CCL3, CCL4, CCL5, CCL7, and CCL11) were upregulated at 14 dpi in the brain. Furthermore, data revealed differentially regulated analytes across tissues, such as CCL11, CXCL5, and IL-10 in the brain, IL-33/IL-33R in the liver, and VEGF-a and IL-5 in the lungs. Overall, this study provides an overview of the immune dynamics of soluble mediators in congenital CMV.https://www.mdpi.com/1999-4915/16/2/208congenital human cytomegalovirus infectionHCMVMCMVcytokinechemokine |
| spellingShingle | Dubravka Karner Daria Kvestak Berislav Lisnic Maja Cokaric Brdovcak Vanda Juranic Lisnic Paola Kucan Brlic Milena Hasan Tihana Lenac Rovis Comprehensive Analysis of Soluble Mediator Profiles in Congenital CMV Infection Using an MCMV Model Viruses congenital human cytomegalovirus infection HCMV MCMV cytokine chemokine |
| title | Comprehensive Analysis of Soluble Mediator Profiles in Congenital CMV Infection Using an MCMV Model |
| title_full | Comprehensive Analysis of Soluble Mediator Profiles in Congenital CMV Infection Using an MCMV Model |
| title_fullStr | Comprehensive Analysis of Soluble Mediator Profiles in Congenital CMV Infection Using an MCMV Model |
| title_full_unstemmed | Comprehensive Analysis of Soluble Mediator Profiles in Congenital CMV Infection Using an MCMV Model |
| title_short | Comprehensive Analysis of Soluble Mediator Profiles in Congenital CMV Infection Using an MCMV Model |
| title_sort | comprehensive analysis of soluble mediator profiles in congenital cmv infection using an mcmv model |
| topic | congenital human cytomegalovirus infection HCMV MCMV cytokine chemokine |
| url | https://www.mdpi.com/1999-4915/16/2/208 |
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