Genetic Dissection of Light-Regulated Adventitious Root Induction in <i>Arabidopsis thaliana</i> Hypocotyls
Photomorphogenic responses of etiolated seedlings include the inhibition of hypocotyl elongation and opening of the apical hook. In addition, dark-grown seedlings respond to light by the formation of adventitious roots (AR) on the hypocotyl. How light signaling controls adventitious rooting is less...
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/10/5301 |
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| author | Yinwei Zeng Sebastien Schotte Hoang Khai Trinh Inge Verstraeten Jing Li Ellen Van de Velde Steffen Vanneste Danny Geelen |
| author_facet | Yinwei Zeng Sebastien Schotte Hoang Khai Trinh Inge Verstraeten Jing Li Ellen Van de Velde Steffen Vanneste Danny Geelen |
| author_sort | Yinwei Zeng |
| collection | DOAJ |
| description | Photomorphogenic responses of etiolated seedlings include the inhibition of hypocotyl elongation and opening of the apical hook. In addition, dark-grown seedlings respond to light by the formation of adventitious roots (AR) on the hypocotyl. How light signaling controls adventitious rooting is less well understood. Hereto, we analyzed adventitious rooting under different light conditions in wild type and photomorphogenesis mutants in <i>Arabidopsis thaliana</i>. Etiolation was not essential for AR formation but raised the competence to form AR under white and blue light. The blue light receptors CRY1 and PHOT1/PHOT2 are key elements contributing to the induction of AR formation in response to light. Furthermore, etiolation-controlled competence for AR formation depended on the COP9 signalosome, E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC (COP1), the COP1 interacting SUPPRESSOR OF PHYA-105 (SPA) kinase family members (SPA1,2 and 3) and Phytochrome-Interacting Factors (PIF). In contrast, ELONGATED HYPOCOTYL5 (HY5), suppressed AR formation. These findings provide a genetic framework that explains the high and low AR competence of <i>Arabidopsis thaliana</i> hypocotyls that were treated with dark, and light, respectively. We propose that light-induced auxin signal dissipation generates a transient auxin maximum that explains AR induction by a dark to light switch. |
| first_indexed | 2024-03-10T03:46:07Z |
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| id | doaj.art-2871d73a9b4c42809f281cab1bc8cf69 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:46:07Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2871d73a9b4c42809f281cab1bc8cf692023-11-23T11:20:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310530110.3390/ijms23105301Genetic Dissection of Light-Regulated Adventitious Root Induction in <i>Arabidopsis thaliana</i> HypocotylsYinwei Zeng0Sebastien Schotte1Hoang Khai Trinh2Inge Verstraeten3Jing Li4Ellen Van de Velde5Steffen Vanneste6Danny Geelen7Department Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumDepartment Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumDepartment Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumDepartment Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumDepartment Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumDepartment Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumDepartment Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumDepartment Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, BelgiumPhotomorphogenic responses of etiolated seedlings include the inhibition of hypocotyl elongation and opening of the apical hook. In addition, dark-grown seedlings respond to light by the formation of adventitious roots (AR) on the hypocotyl. How light signaling controls adventitious rooting is less well understood. Hereto, we analyzed adventitious rooting under different light conditions in wild type and photomorphogenesis mutants in <i>Arabidopsis thaliana</i>. Etiolation was not essential for AR formation but raised the competence to form AR under white and blue light. The blue light receptors CRY1 and PHOT1/PHOT2 are key elements contributing to the induction of AR formation in response to light. Furthermore, etiolation-controlled competence for AR formation depended on the COP9 signalosome, E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC (COP1), the COP1 interacting SUPPRESSOR OF PHYA-105 (SPA) kinase family members (SPA1,2 and 3) and Phytochrome-Interacting Factors (PIF). In contrast, ELONGATED HYPOCOTYL5 (HY5), suppressed AR formation. These findings provide a genetic framework that explains the high and low AR competence of <i>Arabidopsis thaliana</i> hypocotyls that were treated with dark, and light, respectively. We propose that light-induced auxin signal dissipation generates a transient auxin maximum that explains AR induction by a dark to light switch.https://www.mdpi.com/1422-0067/23/10/5301adventitious roothypocotylphotomorphogenesislight<i>Arabidopsis thaliana</i> |
| spellingShingle | Yinwei Zeng Sebastien Schotte Hoang Khai Trinh Inge Verstraeten Jing Li Ellen Van de Velde Steffen Vanneste Danny Geelen Genetic Dissection of Light-Regulated Adventitious Root Induction in <i>Arabidopsis thaliana</i> Hypocotyls International Journal of Molecular Sciences adventitious root hypocotyl photomorphogenesis light <i>Arabidopsis thaliana</i> |
| title | Genetic Dissection of Light-Regulated Adventitious Root Induction in <i>Arabidopsis thaliana</i> Hypocotyls |
| title_full | Genetic Dissection of Light-Regulated Adventitious Root Induction in <i>Arabidopsis thaliana</i> Hypocotyls |
| title_fullStr | Genetic Dissection of Light-Regulated Adventitious Root Induction in <i>Arabidopsis thaliana</i> Hypocotyls |
| title_full_unstemmed | Genetic Dissection of Light-Regulated Adventitious Root Induction in <i>Arabidopsis thaliana</i> Hypocotyls |
| title_short | Genetic Dissection of Light-Regulated Adventitious Root Induction in <i>Arabidopsis thaliana</i> Hypocotyls |
| title_sort | genetic dissection of light regulated adventitious root induction in i arabidopsis thaliana i hypocotyls |
| topic | adventitious root hypocotyl photomorphogenesis light <i>Arabidopsis thaliana</i> |
| url | https://www.mdpi.com/1422-0067/23/10/5301 |
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