Molecular characterization and expression pattern of tobacco (Nicotiana tabacum) ascorbate oxidase gene
The complete coding sequence of one tobacco (Nicotiana tabacum) gene, ascorbate oxidase (AO), was isolated by the application of reverse transcription-polymerase chain reaction. The tobacco AO gene consists of a 1722-bp open reading frame and encodes a protein of 573 amino acids. Sequence comparison...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2016-11-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2016.1231594 |
| _version_ | 1811274078845140992 |
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| author | Zhihua Li Jian Chen Chao Wang Yunjie Huang Jiajun Wang Shifei Dong Lu Tang Jiani Liu Zebin Chen Dingkang Wang Shengguang Xu Lei Yu |
| author_facet | Zhihua Li Jian Chen Chao Wang Yunjie Huang Jiajun Wang Shifei Dong Lu Tang Jiani Liu Zebin Chen Dingkang Wang Shengguang Xu Lei Yu |
| author_sort | Zhihua Li |
| collection | DOAJ |
| description | The complete coding sequence of one tobacco (Nicotiana tabacum) gene, ascorbate oxidase (AO), was isolated by the application of reverse transcription-polymerase chain reaction. The tobacco AO gene consists of a 1722-bp open reading frame and encodes a protein of 573 amino acids. Sequence comparison analysis revealed that the tobacco AO protein shares high homology with the AO proteins of Lycopersicon esculentum (89%), Populus trichocarpa (75%), soybean (74%), castor bean (73%) and peach (73%). The prediction of transmembrane helices showed that tobacco AO might be a transmembrane protein. The expression profile was studied and the results indicated that the tobacco AO gene was diversely expressed in different tobacco tissues, including leaves, stem, roots and flowers. Our experiment laid the grounds for further research on this tobacco gene. |
| first_indexed | 2024-04-12T23:12:27Z |
| format | Article |
| id | doaj.art-28749f9cf8e848a69fd2b2497ed7ff2d |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-04-12T23:12:27Z |
| publishDate | 2016-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-28749f9cf8e848a69fd2b2497ed7ff2d2022-12-22T03:12:46ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302016-11-013061059106410.1080/13102818.2016.12315941231594Molecular characterization and expression pattern of tobacco (Nicotiana tabacum) ascorbate oxidase geneZhihua Li0Jian Chen1Chao Wang2Yunjie Huang3Jiajun Wang4Shifei Dong5Lu Tang6Jiani Liu7Zebin Chen8Dingkang Wang9Shengguang Xu10Lei Yu11Hongyun Honghe Tobacco (Group) Co., Ltd.Technology Center of Yunnan Tobacco Industry Co., Ltd.Hongyun Honghe Tobacco (Group) Co., Ltd.Technology Center of Yunnan Tobacco Industry Co., Ltd.Technology Center of Yunnan Tobacco Industry Co., Ltd.Hongyun Honghe Tobacco (Group) Co., Ltd.Hongyun Honghe Tobacco (Group) Co., Ltd.Kunming UniversityKunming UniversityKunming UniversityKunming UniversityKunming UniversityThe complete coding sequence of one tobacco (Nicotiana tabacum) gene, ascorbate oxidase (AO), was isolated by the application of reverse transcription-polymerase chain reaction. The tobacco AO gene consists of a 1722-bp open reading frame and encodes a protein of 573 amino acids. Sequence comparison analysis revealed that the tobacco AO protein shares high homology with the AO proteins of Lycopersicon esculentum (89%), Populus trichocarpa (75%), soybean (74%), castor bean (73%) and peach (73%). The prediction of transmembrane helices showed that tobacco AO might be a transmembrane protein. The expression profile was studied and the results indicated that the tobacco AO gene was diversely expressed in different tobacco tissues, including leaves, stem, roots and flowers. Our experiment laid the grounds for further research on this tobacco gene.http://dx.doi.org/10.1080/13102818.2016.1231594TobaccogeneAOexpression pattern |
| spellingShingle | Zhihua Li Jian Chen Chao Wang Yunjie Huang Jiajun Wang Shifei Dong Lu Tang Jiani Liu Zebin Chen Dingkang Wang Shengguang Xu Lei Yu Molecular characterization and expression pattern of tobacco (Nicotiana tabacum) ascorbate oxidase gene Biotechnology & Biotechnological Equipment Tobacco gene AO expression pattern |
| title | Molecular characterization and expression pattern of tobacco (Nicotiana tabacum) ascorbate oxidase gene |
| title_full | Molecular characterization and expression pattern of tobacco (Nicotiana tabacum) ascorbate oxidase gene |
| title_fullStr | Molecular characterization and expression pattern of tobacco (Nicotiana tabacum) ascorbate oxidase gene |
| title_full_unstemmed | Molecular characterization and expression pattern of tobacco (Nicotiana tabacum) ascorbate oxidase gene |
| title_short | Molecular characterization and expression pattern of tobacco (Nicotiana tabacum) ascorbate oxidase gene |
| title_sort | molecular characterization and expression pattern of tobacco nicotiana tabacum ascorbate oxidase gene |
| topic | Tobacco gene AO expression pattern |
| url | http://dx.doi.org/10.1080/13102818.2016.1231594 |
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