Unusually high incidence of polyomavirus JC infection in the higher grade of colorectal cancer tissues in Taiwan
Abstract Introduction The human JC polyomavirus (JCPyV) has been detected in colorectal cancer (CRC) tissues and is suggested to contribute to CRC tumorigenesis. The rearrangement of the JCPyV regulatory region is supposedly associated with CRC development. The progression of CRC involves the stepwi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-07-01
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| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-022-00756-2 |
| _version_ | 1811287228204187648 |
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| author | Chuan-Yin Fang San-Yuan Chen Bo-Xiu Hsiao Hsin-Yi Huang Yi-Ju Chen Chun-Liang Tung Chiung-Yao Fang |
| author_facet | Chuan-Yin Fang San-Yuan Chen Bo-Xiu Hsiao Hsin-Yi Huang Yi-Ju Chen Chun-Liang Tung Chiung-Yao Fang |
| author_sort | Chuan-Yin Fang |
| collection | DOAJ |
| description | Abstract Introduction The human JC polyomavirus (JCPyV) has been detected in colorectal cancer (CRC) tissues and is suggested to contribute to CRC tumorigenesis. The rearrangement of the JCPyV regulatory region is supposedly associated with CRC development. The progression of CRC involves the stepwise accumulation of mutations. The large tumor antigen (LT) of JCPyV can trigger uncontrolled cell cycle progression by targeting oncogenes, and tumor suppressor genes, and causing chromosome instability. Few studies have focused on the presence of JCPyV DNA in the higher grade of CRC tissues. Methods We collected 95 tissue blocks from samples of stages I, II, III, and IV CRC. Nested PCR targeting the regulatory region of the viral genome was performed to determine the presence of JCPyV DNA in the various stages of colorectal cancer tissues. Results The nested PCR results showed that the positive rate of JCPyV DNA increased with the progression of CRC stages. The archetypal-like, non-rearrangement genotype of JCPyV with subtle mutations was the major genotype found in CRC samples. Conclusions This finding in our study suggests that there may be an association between JCPyV and CRC progression. |
| first_indexed | 2024-04-13T03:15:02Z |
| format | Article |
| id | doaj.art-2876ff46561a47dd8b8f6f9d44f7ec52 |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-04-13T03:15:02Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-2876ff46561a47dd8b8f6f9d44f7ec522022-12-22T03:04:56ZengBMCEuropean Journal of Medical Research2047-783X2022-07-012711810.1186/s40001-022-00756-2Unusually high incidence of polyomavirus JC infection in the higher grade of colorectal cancer tissues in TaiwanChuan-Yin Fang0San-Yuan Chen1Bo-Xiu Hsiao2Hsin-Yi Huang3Yi-Ju Chen4Chun-Liang Tung5Chiung-Yao Fang6Division of Colon and Rectal Surgery, Ditmanson Medical Foundation Chia-Yi Christian HospitalDepartment of Chinese Medicine, Ditmanson Medical Foundation Chiayi Christian HospitalDepartment of Chinese Medicine, Ditmanson Medical Foundation Chiayi Christian HospitalDepartment of Medical Research, Ditmanson Medical Foundation Chiayi Christian HospitalDepartment of Pathology, Ditmanson Medical Foundation Chiayi Christian HospitalDepartment of Pathology, Ditmanson Medical Foundation Chiayi Christian HospitalDepartment of Medical Research, Ditmanson Medical Foundation Chiayi Christian HospitalAbstract Introduction The human JC polyomavirus (JCPyV) has been detected in colorectal cancer (CRC) tissues and is suggested to contribute to CRC tumorigenesis. The rearrangement of the JCPyV regulatory region is supposedly associated with CRC development. The progression of CRC involves the stepwise accumulation of mutations. The large tumor antigen (LT) of JCPyV can trigger uncontrolled cell cycle progression by targeting oncogenes, and tumor suppressor genes, and causing chromosome instability. Few studies have focused on the presence of JCPyV DNA in the higher grade of CRC tissues. Methods We collected 95 tissue blocks from samples of stages I, II, III, and IV CRC. Nested PCR targeting the regulatory region of the viral genome was performed to determine the presence of JCPyV DNA in the various stages of colorectal cancer tissues. Results The nested PCR results showed that the positive rate of JCPyV DNA increased with the progression of CRC stages. The archetypal-like, non-rearrangement genotype of JCPyV with subtle mutations was the major genotype found in CRC samples. Conclusions This finding in our study suggests that there may be an association between JCPyV and CRC progression.https://doi.org/10.1186/s40001-022-00756-2JCPyVColorectal cancerNested PCRTumor progressionDNA virus |
| spellingShingle | Chuan-Yin Fang San-Yuan Chen Bo-Xiu Hsiao Hsin-Yi Huang Yi-Ju Chen Chun-Liang Tung Chiung-Yao Fang Unusually high incidence of polyomavirus JC infection in the higher grade of colorectal cancer tissues in Taiwan European Journal of Medical Research JCPyV Colorectal cancer Nested PCR Tumor progression DNA virus |
| title | Unusually high incidence of polyomavirus JC infection in the higher grade of colorectal cancer tissues in Taiwan |
| title_full | Unusually high incidence of polyomavirus JC infection in the higher grade of colorectal cancer tissues in Taiwan |
| title_fullStr | Unusually high incidence of polyomavirus JC infection in the higher grade of colorectal cancer tissues in Taiwan |
| title_full_unstemmed | Unusually high incidence of polyomavirus JC infection in the higher grade of colorectal cancer tissues in Taiwan |
| title_short | Unusually high incidence of polyomavirus JC infection in the higher grade of colorectal cancer tissues in Taiwan |
| title_sort | unusually high incidence of polyomavirus jc infection in the higher grade of colorectal cancer tissues in taiwan |
| topic | JCPyV Colorectal cancer Nested PCR Tumor progression DNA virus |
| url | https://doi.org/10.1186/s40001-022-00756-2 |
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