Effects of Long-Term (17 Years) Nitrogen Input on Soil Bacterial Community in Sanjiang Plain: The Largest Marsh Wetland in China
Increased nitrogen (N) input from natural factors and human activities may negatively impact the health of marsh wetlands. However, the understanding of how exogenous N affects the ecosystem remains limited. We selected the soil bacterial community as the index of ecosystem health and performed a lo...
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MDPI AG
2023-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1552 |
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| author | Zhenbo Chen Chi Zhang Zhihong Liu Changchun Song Shuai Xin |
| author_facet | Zhenbo Chen Chi Zhang Zhihong Liu Changchun Song Shuai Xin |
| author_sort | Zhenbo Chen |
| collection | DOAJ |
| description | Increased nitrogen (N) input from natural factors and human activities may negatively impact the health of marsh wetlands. However, the understanding of how exogenous N affects the ecosystem remains limited. We selected the soil bacterial community as the index of ecosystem health and performed a long-term N input experiment, including four N levels of 0, 6, 12, and 24 gN·m<sup>−2</sup>·a<sup>−1</sup> (denoted as CK, C1, C2, and C3, respectively). The results showed that a high-level N (24 gN·m<sup>−2</sup>·a<sup>−1</sup>) input could significantly reduce the Chao index and ACE index for the bacterial community and inhibit some dominant microorganisms. The RDA results indicated that TN and NH<sub>4</sub><sup>+</sup> were the critical factors influencing the soil microbial community under the long-term N input. Moreover, the long-term N input was found to significantly reduce the abundance of <i>Azospirillum</i> and <i>Desulfovibrio</i>, which were typical N-fixing microorganisms. Conversely, the long-term N input was found to significantly increase the abundance of <i>Nitrosospira</i> and <i>Clostridium_sensu_stricto_1</i>, which were typical nitrifying and denitrifying microorganisms. Increased soil N content has been suggested to inhibit the N fixation function of the wetland and exert a positive effect on the processes of nitrification and denitrification in the wetland ecosystem. Our research can be used to improve strategies to protect wetland health. |
| first_indexed | 2024-03-11T02:08:02Z |
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| id | doaj.art-287777134aa149c7b01c4d1c2efb0325 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T02:08:02Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-287777134aa149c7b01c4d1c2efb03252023-11-18T11:43:28ZengMDPI AGMicroorganisms2076-26072023-06-01116155210.3390/microorganisms11061552Effects of Long-Term (17 Years) Nitrogen Input on Soil Bacterial Community in Sanjiang Plain: The Largest Marsh Wetland in ChinaZhenbo Chen0Chi Zhang1Zhihong Liu2Changchun Song3Shuai Xin4Faculty of Infrastructure Engineering, Dalian University of Technology, Dalian 116023, ChinaFaculty of Infrastructure Engineering, Dalian University of Technology, Dalian 116023, ChinaFaculty of Infrastructure Engineering, Dalian University of Technology, Dalian 116023, ChinaFaculty of Infrastructure Engineering, Dalian University of Technology, Dalian 116023, ChinaFaculty of Infrastructure Engineering, Dalian University of Technology, Dalian 116023, ChinaIncreased nitrogen (N) input from natural factors and human activities may negatively impact the health of marsh wetlands. However, the understanding of how exogenous N affects the ecosystem remains limited. We selected the soil bacterial community as the index of ecosystem health and performed a long-term N input experiment, including four N levels of 0, 6, 12, and 24 gN·m<sup>−2</sup>·a<sup>−1</sup> (denoted as CK, C1, C2, and C3, respectively). The results showed that a high-level N (24 gN·m<sup>−2</sup>·a<sup>−1</sup>) input could significantly reduce the Chao index and ACE index for the bacterial community and inhibit some dominant microorganisms. The RDA results indicated that TN and NH<sub>4</sub><sup>+</sup> were the critical factors influencing the soil microbial community under the long-term N input. Moreover, the long-term N input was found to significantly reduce the abundance of <i>Azospirillum</i> and <i>Desulfovibrio</i>, which were typical N-fixing microorganisms. Conversely, the long-term N input was found to significantly increase the abundance of <i>Nitrosospira</i> and <i>Clostridium_sensu_stricto_1</i>, which were typical nitrifying and denitrifying microorganisms. Increased soil N content has been suggested to inhibit the N fixation function of the wetland and exert a positive effect on the processes of nitrification and denitrification in the wetland ecosystem. Our research can be used to improve strategies to protect wetland health.https://www.mdpi.com/2076-2607/11/6/1552soil microorganismslong termnitrogen inputnitrogen cyclewetland |
| spellingShingle | Zhenbo Chen Chi Zhang Zhihong Liu Changchun Song Shuai Xin Effects of Long-Term (17 Years) Nitrogen Input on Soil Bacterial Community in Sanjiang Plain: The Largest Marsh Wetland in China Microorganisms soil microorganisms long term nitrogen input nitrogen cycle wetland |
| title | Effects of Long-Term (17 Years) Nitrogen Input on Soil Bacterial Community in Sanjiang Plain: The Largest Marsh Wetland in China |
| title_full | Effects of Long-Term (17 Years) Nitrogen Input on Soil Bacterial Community in Sanjiang Plain: The Largest Marsh Wetland in China |
| title_fullStr | Effects of Long-Term (17 Years) Nitrogen Input on Soil Bacterial Community in Sanjiang Plain: The Largest Marsh Wetland in China |
| title_full_unstemmed | Effects of Long-Term (17 Years) Nitrogen Input on Soil Bacterial Community in Sanjiang Plain: The Largest Marsh Wetland in China |
| title_short | Effects of Long-Term (17 Years) Nitrogen Input on Soil Bacterial Community in Sanjiang Plain: The Largest Marsh Wetland in China |
| title_sort | effects of long term 17 years nitrogen input on soil bacterial community in sanjiang plain the largest marsh wetland in china |
| topic | soil microorganisms long term nitrogen input nitrogen cycle wetland |
| url | https://www.mdpi.com/2076-2607/11/6/1552 |
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