The expression of the SCD1 gene and its correlation with fattening and carcass traits in sheep
Stearoyl-CoA desaturase 1 (SCD1) is a critical enzyme that catalyzes the synthesis of monounsaturated fatty acids and is involved in several signaling pathways related to lipid metabolism. The objective of the present study was to estimate the expression of the SCD1 gene in three different ovine tis...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2016-01-01
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| Series: | Archives Animal Breeding |
| Online Access: | http://www.arch-anim-breed.net/59/37/2016/aab-59-37-2016.pdf |
| Summary: | Stearoyl-CoA desaturase 1 (SCD1) is a critical enzyme that catalyzes the synthesis
of monounsaturated fatty acids and is involved in several signaling
pathways related to lipid metabolism. The objective of the present study was
to estimate the expression of the SCD1 gene in three different ovine tissues
strongly associated with lipid homeostasis. The SCD1 gene expression measurement
was performed on three tissues (liver, subcutaneous fat, perirenal fat)
originated from 15 old-type Polish Merino sheep. The SCD1 transcript abundance
was evaluated based on the two most stable endogenous controls (RPS2 –
ribosomal protein S2; ATP5G2 – H(+)-transporting ATP
synthase). The highest expression of the SCD1 gene was observed in ovine
subcutaneous fat compared to perirenal fat and liver. Furthermore, the
present research indicated the significant correlation between ovine SCD1
transcript abundance and several important production traits. The expression
of the SCD1 gene in liver and perirenal fat highly positively correlated with
the feed : gain ratio, test of daily gain and age of the animals at slaughter.
Moreover, in both tissues, the SCD1mRNA level positively correlated with weight
and content of perirenal fat and subcutaneous fat (<i>R</i> = 0.64, 0.8, 0.6,
respectively) and negatively with assessment of external fat content with
the use of the EUROP scale (<i>R</i> = −0.64). The SCD1 expression in subcutaneous fat
also corresponds with back fat of blade chop and thickness of longissimus dorsi muscles
evaluated using USG (ultrasonography) (<i>R</i> = −0.6 and 0.62, respectively). The
significant correlation between SCD1 transcript abundance and fattening and
slaughtering traits indicate the ability to improve important production
traits in sheep via modification of expression of the SCD1
gene. |
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| ISSN: | 0003-9438 2363-9822 |