Syngeneic B16F10 Melanoma Causes Cachexia and Impaired Skeletal Muscle Strength and Locomotor Activity in Mice
Muscle wasting has been emerging as one of the principal components of cancer cachexia, leading to progressive impairment of work capacity. Despite early stages melanomas rarely promotes weight loss, the appearance of metastatic and/or solid tumor melanoma can leads to cachexia development. Here, we...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-09-01
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| Series: | Frontiers in Physiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2017.00715/full |
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| author | Fabrício A. Voltarelli Fabrício A. Voltarelli Fernando T. Frajacomo Fernando T. Frajacomo Camila de Souza Padilha Mayra T. J. Testa Paola S. Cella Diogo F. Ribeiro Donizete X. de Oliveira Luciana C. Veronez Gabriela S. Bisson Felipe A. Moura Rafael Deminice |
| author_facet | Fabrício A. Voltarelli Fabrício A. Voltarelli Fernando T. Frajacomo Fernando T. Frajacomo Camila de Souza Padilha Mayra T. J. Testa Paola S. Cella Diogo F. Ribeiro Donizete X. de Oliveira Luciana C. Veronez Gabriela S. Bisson Felipe A. Moura Rafael Deminice |
| author_sort | Fabrício A. Voltarelli |
| collection | DOAJ |
| description | Muscle wasting has been emerging as one of the principal components of cancer cachexia, leading to progressive impairment of work capacity. Despite early stages melanomas rarely promotes weight loss, the appearance of metastatic and/or solid tumor melanoma can leads to cachexia development. Here, we investigated the B16F10 tumor-induced cachexia and its contribution to muscle strength and locomotor-like activity impairment. C57BL/6 mice were subcutaneously injected with 5 × 104 B16F10 melanoma cells or PBS as a Sham negative control. Tumor growth was monitored during a period of 28 days. Compared to Sham mice, tumor group depicts a loss of skeletal muscle, as well as significantly reduced muscle grip strength and epididymal fat mass. This data are in agreement with mild to severe catabolic host response promoted by elevated serum tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and lactate dehydrogenase (LDH) activity. Tumor implantation has also compromised general locomotor activity and decreased exploratory behavior. Likewise, muscle loss, and elevated inflammatory interleukin were associated to muscle strength loss and locomotor activity impairment. In conclusion, our data demonstrated that subcutaneous B16F10 melanoma tumor-driven catabolic state in response to a pro-inflammatory environment that is associated with impaired skeletal muscle strength and decreased locomotor activity in tumor-bearing mice. |
| first_indexed | 2024-04-13T13:31:12Z |
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| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-13T13:31:12Z |
| publishDate | 2017-09-01 |
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| series | Frontiers in Physiology |
| spelling | doaj.art-287b11af598e4ade8508f267ce9a1a142022-12-22T02:44:58ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2017-09-01810.3389/fphys.2017.00715280108Syngeneic B16F10 Melanoma Causes Cachexia and Impaired Skeletal Muscle Strength and Locomotor Activity in MiceFabrício A. Voltarelli0Fabrício A. Voltarelli1Fernando T. Frajacomo2Fernando T. Frajacomo3Camila de Souza Padilha4Mayra T. J. Testa5Paola S. Cella6Diogo F. Ribeiro7Donizete X. de Oliveira8Luciana C. Veronez9Gabriela S. Bisson10Felipe A. Moura11Rafael Deminice12Department of Physical Education, Faculty of Physical Education and Sport, State University of LondrinaLondrina, BrazilDepartment of Physical Education, Faculty of Physical Education, Federal University of Mato GrossoCuiabá, BrazilDepartment of Physical Education, Faculty of Physical Education and Sport, State University of LondrinaLondrina, BrazilProgram of Molecular Carcinogenesis, Brazilian National Institute of CancerRio de Janeiro, BrazilDepartment of Physical Education, Faculty of Physical Education and Sport, State University of LondrinaLondrina, BrazilDepartment of Physical Education, Faculty of Physical Education and Sport, State University of LondrinaLondrina, BrazilDepartment of Physical Education, Faculty of Physical Education and Sport, State University of LondrinaLondrina, BrazilDepartment of Physical Education, Faculty of Physical Education and Sport, State University of LondrinaLondrina, BrazilDepartment of Physical Education, Faculty of Physical Education and Sport, State University of LondrinaLondrina, BrazilDepartment of Maternal-Infant Nursing and Public Health, Ribeirao Preto College of Nursing, University of São PauloSão Paulo, BrazilDepartment of Maternal-Infant Nursing and Public Health, Ribeirao Preto College of Nursing, University of São PauloSão Paulo, BrazilDepartment of Physical Education, Faculty of Physical Education and Sport, State University of LondrinaLondrina, BrazilDepartment of Physical Education, Faculty of Physical Education and Sport, State University of LondrinaLondrina, BrazilMuscle wasting has been emerging as one of the principal components of cancer cachexia, leading to progressive impairment of work capacity. Despite early stages melanomas rarely promotes weight loss, the appearance of metastatic and/or solid tumor melanoma can leads to cachexia development. Here, we investigated the B16F10 tumor-induced cachexia and its contribution to muscle strength and locomotor-like activity impairment. C57BL/6 mice were subcutaneously injected with 5 × 104 B16F10 melanoma cells or PBS as a Sham negative control. Tumor growth was monitored during a period of 28 days. Compared to Sham mice, tumor group depicts a loss of skeletal muscle, as well as significantly reduced muscle grip strength and epididymal fat mass. This data are in agreement with mild to severe catabolic host response promoted by elevated serum tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and lactate dehydrogenase (LDH) activity. Tumor implantation has also compromised general locomotor activity and decreased exploratory behavior. Likewise, muscle loss, and elevated inflammatory interleukin were associated to muscle strength loss and locomotor activity impairment. In conclusion, our data demonstrated that subcutaneous B16F10 melanoma tumor-driven catabolic state in response to a pro-inflammatory environment that is associated with impaired skeletal muscle strength and decreased locomotor activity in tumor-bearing mice.http://journal.frontiersin.org/article/10.3389/fphys.2017.00715/fullcancermelanomainflammationmuscle strengthgeneral locomotor activity |
| spellingShingle | Fabrício A. Voltarelli Fabrício A. Voltarelli Fernando T. Frajacomo Fernando T. Frajacomo Camila de Souza Padilha Mayra T. J. Testa Paola S. Cella Diogo F. Ribeiro Donizete X. de Oliveira Luciana C. Veronez Gabriela S. Bisson Felipe A. Moura Rafael Deminice Syngeneic B16F10 Melanoma Causes Cachexia and Impaired Skeletal Muscle Strength and Locomotor Activity in Mice Frontiers in Physiology cancer melanoma inflammation muscle strength general locomotor activity |
| title | Syngeneic B16F10 Melanoma Causes Cachexia and Impaired Skeletal Muscle Strength and Locomotor Activity in Mice |
| title_full | Syngeneic B16F10 Melanoma Causes Cachexia and Impaired Skeletal Muscle Strength and Locomotor Activity in Mice |
| title_fullStr | Syngeneic B16F10 Melanoma Causes Cachexia and Impaired Skeletal Muscle Strength and Locomotor Activity in Mice |
| title_full_unstemmed | Syngeneic B16F10 Melanoma Causes Cachexia and Impaired Skeletal Muscle Strength and Locomotor Activity in Mice |
| title_short | Syngeneic B16F10 Melanoma Causes Cachexia and Impaired Skeletal Muscle Strength and Locomotor Activity in Mice |
| title_sort | syngeneic b16f10 melanoma causes cachexia and impaired skeletal muscle strength and locomotor activity in mice |
| topic | cancer melanoma inflammation muscle strength general locomotor activity |
| url | http://journal.frontiersin.org/article/10.3389/fphys.2017.00715/full |
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