Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles
Supported lipid membranes are widely used platforms which serve as simplified models of cell membranes. Among numerous methods used for preparation of planar lipid films, self-assembly of bicelles appears to be promising strategy. Therefore, in this paper we have examined the mechanism of formation...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/11/1/11 |
| _version_ | 1797543684387897344 |
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| author | Damian Dziubak Kamil Strzelak Slawomir Sek |
| author_facet | Damian Dziubak Kamil Strzelak Slawomir Sek |
| author_sort | Damian Dziubak |
| collection | DOAJ |
| description | Supported lipid membranes are widely used platforms which serve as simplified models of cell membranes. Among numerous methods used for preparation of planar lipid films, self-assembly of bicelles appears to be promising strategy. Therefore, in this paper we have examined the mechanism of formation and the electrochemical properties of lipid films deposited onto thioglucose-modified gold electrodes from bicellar mixtures. It was found that adsorption of the bicelles occurs by replacement of interfacial water and it leads to formation of a double bilayer structure on the electrode surface. The resulting lipid assembly contains numerous defects and pinholes which affect the permeability of the membrane for ions and water. Significant improvement in morphology and electrochemical characteristics is achieved upon freeze–thaw treatment of the deposited membrane. The lipid assembly is rearranged to single bilayer configuration with locally occurring patches of the second bilayer, and the number of pinholes is substantially decreased. Electrochemical characterization of the lipid membrane after freeze–thaw treatment demonstrated that its permeability for ions and water is significantly reduced, which was manifested by the relatively high value of the membrane resistance. |
| first_indexed | 2024-03-10T13:49:09Z |
| format | Article |
| id | doaj.art-287c6a8326bc4b85937c5923b538873b |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-10T13:49:09Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-287c6a8326bc4b85937c5923b538873b2023-11-21T02:17:33ZengMDPI AGMembranes2077-03752020-12-011111110.3390/membranes11010011Electrochemical Properties of Lipid Membranes Self-Assembled from BicellesDamian Dziubak0Kamil Strzelak1Slawomir Sek2Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089 Warsaw, PolandFaculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, PolandFaculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089 Warsaw, PolandSupported lipid membranes are widely used platforms which serve as simplified models of cell membranes. Among numerous methods used for preparation of planar lipid films, self-assembly of bicelles appears to be promising strategy. Therefore, in this paper we have examined the mechanism of formation and the electrochemical properties of lipid films deposited onto thioglucose-modified gold electrodes from bicellar mixtures. It was found that adsorption of the bicelles occurs by replacement of interfacial water and it leads to formation of a double bilayer structure on the electrode surface. The resulting lipid assembly contains numerous defects and pinholes which affect the permeability of the membrane for ions and water. Significant improvement in morphology and electrochemical characteristics is achieved upon freeze–thaw treatment of the deposited membrane. The lipid assembly is rearranged to single bilayer configuration with locally occurring patches of the second bilayer, and the number of pinholes is substantially decreased. Electrochemical characterization of the lipid membrane after freeze–thaw treatment demonstrated that its permeability for ions and water is significantly reduced, which was manifested by the relatively high value of the membrane resistance.https://www.mdpi.com/2077-0375/11/1/11electrochemistrygold electrodesupported lipid membranesbicellesself-assembly |
| spellingShingle | Damian Dziubak Kamil Strzelak Slawomir Sek Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles Membranes electrochemistry gold electrode supported lipid membranes bicelles self-assembly |
| title | Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles |
| title_full | Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles |
| title_fullStr | Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles |
| title_full_unstemmed | Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles |
| title_short | Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles |
| title_sort | electrochemical properties of lipid membranes self assembled from bicelles |
| topic | electrochemistry gold electrode supported lipid membranes bicelles self-assembly |
| url | https://www.mdpi.com/2077-0375/11/1/11 |
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