HLA-C Genotyping Reveals Haplotype C*07 as a Potential Biomarker of Late Psoriasis Onset in Moroccan Patients
Psoriasis still has an unknown etiology. Genetic predisposition shows the association between HLA-Cw6 allele and psoriasis. Although biotherapies have been proven effective in psoriasis treatment, methotrexate (MTX) is still used as a first-line systemic therapy due to its efficacy/affordability, bu...
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MDPI AG
2023-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/2/66 |
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| author | Chaimaa Benlabsir Myriam Riyad Imane El Idrissi Saik Hanaa Ettayebi Oussama Aazzane Kawtar Nassar Soukaina Zaher Siham Bennani Brahim Admou Samy Housbane Khalid Sadki Soumiya Chiheb Hassan Fellah |
| author_facet | Chaimaa Benlabsir Myriam Riyad Imane El Idrissi Saik Hanaa Ettayebi Oussama Aazzane Kawtar Nassar Soukaina Zaher Siham Bennani Brahim Admou Samy Housbane Khalid Sadki Soumiya Chiheb Hassan Fellah |
| author_sort | Chaimaa Benlabsir |
| collection | DOAJ |
| description | Psoriasis still has an unknown etiology. Genetic predisposition shows the association between HLA-Cw6 allele and psoriasis. Although biotherapies have been proven effective in psoriasis treatment, methotrexate (MTX) is still used as a first-line systemic therapy due to its efficacy/affordability, but the differential response to MTX is mostly related to interindividual genetic variability and remains an issue. Our study aimed to analyze HLA-C allele frequencies in a sample of Moroccan psoriatic patients and assess the therapeutic response to MTX. Whole blood of 54 Moroccan psoriatic patients was collected and DNA was extracted. Patients’ HLA-C locus was genotyped by PCR-SSO. Results were analyzed with Luminex xMAP Technology and Match-it DNA Evolution 3.4. HLA-C typing results of 77 sex- and age-matched unrelated non-psoriatic healthy subjects were included. We observed no difference in the allelic distribution of HLA-C between patients and healthy controls, suggesting that none of the HLA-C alleles were significantly associated with psoriasis. Moreover, the HLA-C*07 allele was associated with a late age at disease onset (>30 years old) (<i>p</i> = 0.007). No statistically significant association was found between HLA-C allele expression and response to MTX, despite a higher frequency of HLA-C*06 in responders compared to non-responders. Thus, HLA-C*07 could be a biomarker of late psoriasis onset in the Moroccan population. |
| first_indexed | 2024-03-11T08:58:41Z |
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| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T08:58:41Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-287d47bb630847ce9faf9da190dd70152023-11-16T19:50:37ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-01-014521012102310.3390/cimb45020066HLA-C Genotyping Reveals Haplotype C*07 as a Potential Biomarker of Late Psoriasis Onset in Moroccan PatientsChaimaa Benlabsir0Myriam Riyad1Imane El Idrissi Saik2Hanaa Ettayebi3Oussama Aazzane4Kawtar Nassar5Soukaina Zaher6Siham Bennani7Brahim Admou8Samy Housbane9Khalid Sadki10Soumiya Chiheb11Hassan Fellah12Laboratory of Cellular and Molecular Pathology, Research Team on Immunopathology of Infectious and Systemic Diseases, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Casablanca 20000, MoroccoLaboratory of Cellular and Molecular Pathology, Research Team on Immunopathology of Infectious and Systemic Diseases, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Casablanca 20000, MoroccoLaboratory of Cellular and Molecular Pathology, Research Team on Immunopathology of Infectious and Systemic Diseases, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Casablanca 20000, MoroccoLaboratory of Immunology, Ibn Rochd University Hospital Center, Casablanca 20000, MoroccoLaboratory of Cellular and Molecular Pathology, Research Team on Immunopathology of Infectious and Systemic Diseases, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Casablanca 20000, MoroccoRheumatology Department, Ibn Rochd University Hospital Center, Casablanca 20000, MoroccoRheumatology Department, Ibn Rochd University Hospital Center, Casablanca 20000, MoroccoHLA Typing Laboratory, Pasteur Institute of Morocco, Casablanca 20000, MoroccoLaboratory of Immunology, Center of Clinical Research, University Hospital Mohammed VI, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakesh 40000, MoroccoLaboratory of Cellular and Molecular Pathology, Research Team on Immunopathology of Infectious and Systemic Diseases, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Casablanca 20000, MoroccoOral Biotechnology Laboratory, Fundamental Sciences Department, Faculty of Dental Medicine, Mohammed V University, Rabat 10000, MoroccoLaboratory of Cellular and Molecular Pathology, Research Team on Immunopathology of Infectious and Systemic Diseases, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Casablanca 20000, MoroccoLaboratory of Cellular and Molecular Pathology, Research Team on Immunopathology of Infectious and Systemic Diseases, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Casablanca 20000, MoroccoPsoriasis still has an unknown etiology. Genetic predisposition shows the association between HLA-Cw6 allele and psoriasis. Although biotherapies have been proven effective in psoriasis treatment, methotrexate (MTX) is still used as a first-line systemic therapy due to its efficacy/affordability, but the differential response to MTX is mostly related to interindividual genetic variability and remains an issue. Our study aimed to analyze HLA-C allele frequencies in a sample of Moroccan psoriatic patients and assess the therapeutic response to MTX. Whole blood of 54 Moroccan psoriatic patients was collected and DNA was extracted. Patients’ HLA-C locus was genotyped by PCR-SSO. Results were analyzed with Luminex xMAP Technology and Match-it DNA Evolution 3.4. HLA-C typing results of 77 sex- and age-matched unrelated non-psoriatic healthy subjects were included. We observed no difference in the allelic distribution of HLA-C between patients and healthy controls, suggesting that none of the HLA-C alleles were significantly associated with psoriasis. Moreover, the HLA-C*07 allele was associated with a late age at disease onset (>30 years old) (<i>p</i> = 0.007). No statistically significant association was found between HLA-C allele expression and response to MTX, despite a higher frequency of HLA-C*06 in responders compared to non-responders. Thus, HLA-C*07 could be a biomarker of late psoriasis onset in the Moroccan population.https://www.mdpi.com/1467-3045/45/2/66psoriasisHLA-C allelemethotrexate |
| spellingShingle | Chaimaa Benlabsir Myriam Riyad Imane El Idrissi Saik Hanaa Ettayebi Oussama Aazzane Kawtar Nassar Soukaina Zaher Siham Bennani Brahim Admou Samy Housbane Khalid Sadki Soumiya Chiheb Hassan Fellah HLA-C Genotyping Reveals Haplotype C*07 as a Potential Biomarker of Late Psoriasis Onset in Moroccan Patients Current Issues in Molecular Biology psoriasis HLA-C allele methotrexate |
| title | HLA-C Genotyping Reveals Haplotype C*07 as a Potential Biomarker of Late Psoriasis Onset in Moroccan Patients |
| title_full | HLA-C Genotyping Reveals Haplotype C*07 as a Potential Biomarker of Late Psoriasis Onset in Moroccan Patients |
| title_fullStr | HLA-C Genotyping Reveals Haplotype C*07 as a Potential Biomarker of Late Psoriasis Onset in Moroccan Patients |
| title_full_unstemmed | HLA-C Genotyping Reveals Haplotype C*07 as a Potential Biomarker of Late Psoriasis Onset in Moroccan Patients |
| title_short | HLA-C Genotyping Reveals Haplotype C*07 as a Potential Biomarker of Late Psoriasis Onset in Moroccan Patients |
| title_sort | hla c genotyping reveals haplotype c 07 as a potential biomarker of late psoriasis onset in moroccan patients |
| topic | psoriasis HLA-C allele methotrexate |
| url | https://www.mdpi.com/1467-3045/45/2/66 |
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