Power Spectral Density Analysis of Purkinje Cell Tonic and Burst Firing Patterns From a Rat Model of Ataxia and Riluzole Treated
Introduction: Purkinje Cell (PC) output displays a complex firing pattern consisting of high frequency sodium spikes and low frequency calcium spikes, and disruption in this firing behavior may contribute to cerebellar ataxia. Riluzole, neuroprotective agent, has been demonstrated to have neuroprote...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Iran University of Medical Sciences
2017-01-01
|
| Series: | Basic and Clinical Neuroscience |
| Subjects: | |
| Online Access: | http://bcn.iums.ac.ir/browse.php?a_code=A-10-188-2&slc_lang=en&sid=1 |
| _version_ | 1797284977391435776 |
|---|---|
| author | Samira Abbasi Ataollah Abbasi Yashar Sarbaz Mahyar Janahmadi |
| author_facet | Samira Abbasi Ataollah Abbasi Yashar Sarbaz Mahyar Janahmadi |
| author_sort | Samira Abbasi |
| collection | DOAJ |
| description | Introduction: Purkinje Cell (PC) output displays a complex firing pattern consisting of high frequency sodium spikes and low frequency calcium spikes, and disruption in this firing behavior may contribute to cerebellar ataxia. Riluzole, neuroprotective agent, has been demonstrated to have neuroprotective effects in cerebellar ataxia. Here, the spectral analysis of PCs firing in control, 3-acetylpyridine (3-AP), neurotoxin agent, treated alone and riluzole plus 3-AP treated were investigated to determine changes in the firing properties. Difference in the power spectra of tonic and burst firing was assessed. Furthermore, the role of calcium-activated potassium channels in the power spectra was evaluated.
Methods: Analysis was performed using Matlab. Power spectral density (PSD) of PCs output were obtained. Peak frequencies were extracted from the spectrum and statistical comparisons were done. In addition, a multi-compartment computational model of a Purkinje cell was used. This computational stimulation allowed us to study the changes in the power spectral density of the PC output as a result of alteration in ion channels.
Results: Spectral analysis showed that in the spectrum of tonic and burst firing pattern only high sodium frequency and low calcium frequency was seen, respectively. In addition, there was a significant difference between the frequency components of PCs firing obtained from normal, ataxia and riluzole treated rats. Results indicated that sodium firing frequency of normal, ataxic and treated PCs occurred in approximate frequency of 22.53±5.49, 6.46±0.23, and 31.34±4.07 Hz, respectively; and calcium frequency occurred in frequency of 4.22±2.02, 1.52±1.19, and 3.88±1.37 Hz, respectively. The simulation results demonstrated that blockade of calciumactivated potassium channels in the PC model changed the PSD of the PC model firing activity. This change was similar to PSD changes in ataxia condition.
Conclusion: These alterations in the spectrum of PC output may be a basis for developing possible new treatment strategies to improve cerebellar ataxia. |
| first_indexed | 2024-03-07T17:55:35Z |
| format | Article |
| id | doaj.art-28814d7347ed419487b597c4b65b7e10 |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T17:55:35Z |
| publishDate | 2017-01-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-28814d7347ed419487b597c4b65b7e102024-03-02T12:14:08ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422017-01-01816168Power Spectral Density Analysis of Purkinje Cell Tonic and Burst Firing Patterns From a Rat Model of Ataxia and Riluzole TreatedSamira Abbasi0Ataollah Abbasi1Yashar Sarbaz2Mahyar Janahmadi3 Computational Neuroscience Laboratory, Department of Biomedical Engineering, Faculty of Electrical Engineering, Sahand University of Technology, Tabriz, Iran. Computational Neuroscience Laboratory, Department of Biomedical Engineering, Faculty of Electrical Engineering, Sahand University of Technology, Tabriz, Iran. Department of Mechatronics, School of Engineering- Emerging Technologies, University of Tabriz, Tabriz, Iran. Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Introduction: Purkinje Cell (PC) output displays a complex firing pattern consisting of high frequency sodium spikes and low frequency calcium spikes, and disruption in this firing behavior may contribute to cerebellar ataxia. Riluzole, neuroprotective agent, has been demonstrated to have neuroprotective effects in cerebellar ataxia. Here, the spectral analysis of PCs firing in control, 3-acetylpyridine (3-AP), neurotoxin agent, treated alone and riluzole plus 3-AP treated were investigated to determine changes in the firing properties. Difference in the power spectra of tonic and burst firing was assessed. Furthermore, the role of calcium-activated potassium channels in the power spectra was evaluated. Methods: Analysis was performed using Matlab. Power spectral density (PSD) of PCs output were obtained. Peak frequencies were extracted from the spectrum and statistical comparisons were done. In addition, a multi-compartment computational model of a Purkinje cell was used. This computational stimulation allowed us to study the changes in the power spectral density of the PC output as a result of alteration in ion channels. Results: Spectral analysis showed that in the spectrum of tonic and burst firing pattern only high sodium frequency and low calcium frequency was seen, respectively. In addition, there was a significant difference between the frequency components of PCs firing obtained from normal, ataxia and riluzole treated rats. Results indicated that sodium firing frequency of normal, ataxic and treated PCs occurred in approximate frequency of 22.53±5.49, 6.46±0.23, and 31.34±4.07 Hz, respectively; and calcium frequency occurred in frequency of 4.22±2.02, 1.52±1.19, and 3.88±1.37 Hz, respectively. The simulation results demonstrated that blockade of calciumactivated potassium channels in the PC model changed the PSD of the PC model firing activity. This change was similar to PSD changes in ataxia condition. Conclusion: These alterations in the spectrum of PC output may be a basis for developing possible new treatment strategies to improve cerebellar ataxia. http://bcn.iums.ac.ir/browse.php?a_code=A-10-188-2&slc_lang=en&sid=1Cerebellar ataxias Purkinje cells Calcium spike Sodium spike Signal processing |
| spellingShingle | Samira Abbasi Ataollah Abbasi Yashar Sarbaz Mahyar Janahmadi Power Spectral Density Analysis of Purkinje Cell Tonic and Burst Firing Patterns From a Rat Model of Ataxia and Riluzole Treated Basic and Clinical Neuroscience Cerebellar ataxias Purkinje cells Calcium spike Sodium spike Signal processing |
| title | Power Spectral Density Analysis of Purkinje Cell Tonic and Burst Firing Patterns From a Rat Model of Ataxia and Riluzole Treated |
| title_full | Power Spectral Density Analysis of Purkinje Cell Tonic and Burst Firing Patterns From a Rat Model of Ataxia and Riluzole Treated |
| title_fullStr | Power Spectral Density Analysis of Purkinje Cell Tonic and Burst Firing Patterns From a Rat Model of Ataxia and Riluzole Treated |
| title_full_unstemmed | Power Spectral Density Analysis of Purkinje Cell Tonic and Burst Firing Patterns From a Rat Model of Ataxia and Riluzole Treated |
| title_short | Power Spectral Density Analysis of Purkinje Cell Tonic and Burst Firing Patterns From a Rat Model of Ataxia and Riluzole Treated |
| title_sort | power spectral density analysis of purkinje cell tonic and burst firing patterns from a rat model of ataxia and riluzole treated |
| topic | Cerebellar ataxias Purkinje cells Calcium spike Sodium spike Signal processing |
| url | http://bcn.iums.ac.ir/browse.php?a_code=A-10-188-2&slc_lang=en&sid=1 |
| work_keys_str_mv | AT samiraabbasi powerspectraldensityanalysisofpurkinjecelltonicandburstfiringpatternsfromaratmodelofataxiaandriluzoletreated AT ataollahabbasi powerspectraldensityanalysisofpurkinjecelltonicandburstfiringpatternsfromaratmodelofataxiaandriluzoletreated AT yasharsarbaz powerspectraldensityanalysisofpurkinjecelltonicandburstfiringpatternsfromaratmodelofataxiaandriluzoletreated AT mahyarjanahmadi powerspectraldensityanalysisofpurkinjecelltonicandburstfiringpatternsfromaratmodelofataxiaandriluzoletreated |