Development of propagation technique of indigenous AMF and their inoculation response in citrus
Arbuscular mycorrhizal fungi (AMF) have multi-pronged utility in citrus performance, while their propagation technique is a gap. In this study, indigenous AMF in rhizosphere of Citrus unshiu grafted on trifoliate orange were isolated from fresh root segments (Φ<2 mm), fresh rhizosphere soil (<...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2019-07-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/91696 |
| _version_ | 1828006006647422976 |
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| author | QIANG-SHENG WU JIA-DONG HE A K SRIVASTAVA FEI ZHANG YING-NING ZOU |
| author_facet | QIANG-SHENG WU JIA-DONG HE A K SRIVASTAVA FEI ZHANG YING-NING ZOU |
| author_sort | QIANG-SHENG WU |
| collection | DOAJ |
| description | Arbuscular mycorrhizal fungi (AMF) have multi-pronged utility in citrus performance, while their propagation technique is a gap. In this study, indigenous AMF in rhizosphere of Citrus unshiu grafted on trifoliate orange were isolated from fresh root segments (Φ<2 mm), fresh rhizosphere soil (< 4 mm size), and air-dried rhizosphere soil (< 4 mm size) as AMF-source and propagated with white clover. Subsequently, indigenous AMF inocula were inoculated into potted trifoliate orange to assess the inoculated efficiency. Our results showed that AMF isolated from fresh root segments multiplied by 333.9% significantly higher than those isolated from fresh or air-dried rhizosphere soil. Similar results were obtained with regard to root mycorrhizal colonization (37.16–55.41%) and soil hyphal length (3.88–13.38 cm/g) in trifoliate orange after inoculated with AMF-source from root segments. Mycorrhizal trifoliate orange seedlings carrying AMF inoculum from fresh roots exhibited higher plant growth performance, root morphology, leaf P, K, Mg, Cu and Zn levels, and leaf superoxide dismutase, peroxidase, and catalase activities, compared to non-AMF treatment. Our study, hence, suggested that root segments would be a great choice to propagate indigenous AMF for later inoculating into the rhizosphere of target plants. |
| first_indexed | 2024-04-10T07:50:40Z |
| format | Article |
| id | doaj.art-2886a7359a0143b9935f1fd042c3ce41 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-04-10T07:50:40Z |
| publishDate | 2019-07-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-2886a7359a0143b9935f1fd042c3ce412023-02-23T10:14:07ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192019-07-0189710.56093/ijas.v89i7.91696Development of propagation technique of indigenous AMF and their inoculation response in citrusQIANG-SHENG WU0JIA-DONG HE1A K SRIVASTAVA2FEI ZHANG3YING-NING ZOU4Professor, Yangtze University, Jingzhou, Hubei 434 025, ChinaPostgraduate Student, Yangtze University, Jingzhou, Hubei 434 025, ChinaPrincipal Scientist, ICAR-Central Citrus Research Institute, Nagpur, Maharashtra, IndiaDoctoral Student, Yangtze University, Jingzhou, Hubei 434 025, ChinaAssociate Professor, College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, ChinaArbuscular mycorrhizal fungi (AMF) have multi-pronged utility in citrus performance, while their propagation technique is a gap. In this study, indigenous AMF in rhizosphere of Citrus unshiu grafted on trifoliate orange were isolated from fresh root segments (Φ<2 mm), fresh rhizosphere soil (< 4 mm size), and air-dried rhizosphere soil (< 4 mm size) as AMF-source and propagated with white clover. Subsequently, indigenous AMF inocula were inoculated into potted trifoliate orange to assess the inoculated efficiency. Our results showed that AMF isolated from fresh root segments multiplied by 333.9% significantly higher than those isolated from fresh or air-dried rhizosphere soil. Similar results were obtained with regard to root mycorrhizal colonization (37.16–55.41%) and soil hyphal length (3.88–13.38 cm/g) in trifoliate orange after inoculated with AMF-source from root segments. Mycorrhizal trifoliate orange seedlings carrying AMF inoculum from fresh roots exhibited higher plant growth performance, root morphology, leaf P, K, Mg, Cu and Zn levels, and leaf superoxide dismutase, peroxidase, and catalase activities, compared to non-AMF treatment. Our study, hence, suggested that root segments would be a great choice to propagate indigenous AMF for later inoculating into the rhizosphere of target plants.https://epubs.icar.org.in/index.php/IJAgS/article/view/91696Antioxidant enzymeArbuscular mycorrhizaCitrusRoot morphologySpore |
| spellingShingle | QIANG-SHENG WU JIA-DONG HE A K SRIVASTAVA FEI ZHANG YING-NING ZOU Development of propagation technique of indigenous AMF and their inoculation response in citrus The Indian Journal of Agricultural Sciences Antioxidant enzyme Arbuscular mycorrhiza Citrus Root morphology Spore |
| title | Development of propagation technique of indigenous AMF and their inoculation response in citrus |
| title_full | Development of propagation technique of indigenous AMF and their inoculation response in citrus |
| title_fullStr | Development of propagation technique of indigenous AMF and their inoculation response in citrus |
| title_full_unstemmed | Development of propagation technique of indigenous AMF and their inoculation response in citrus |
| title_short | Development of propagation technique of indigenous AMF and their inoculation response in citrus |
| title_sort | development of propagation technique of indigenous amf and their inoculation response in citrus |
| topic | Antioxidant enzyme Arbuscular mycorrhiza Citrus Root morphology Spore |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/91696 |
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