Evaluation of Toxic Effects Induced by Sub-Acute Exposure to Low Doses of α-Cypermethrin in Adult Male Rats
To contribute new information to the pyrethroid pesticide α-cypermethrin toxicity profile, we evaluated its effects after oral administration to Wistar rats at daily doses of 2.186, 0.015, 0.157, and 0.786 mg/kg bw for 28 days. Evaluations were performed using markers of oxidative stress, cholineste...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/10/12/717 |
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| author | Vilena Kašuba Blanka Tariba Lovaković Ana Lucić Vrdoljak Anja Katić Nevenka Kopjar Vedran Micek Mirta Milić Alica Pizent Davor Želježić Suzana Žunec |
| author_facet | Vilena Kašuba Blanka Tariba Lovaković Ana Lucić Vrdoljak Anja Katić Nevenka Kopjar Vedran Micek Mirta Milić Alica Pizent Davor Želježić Suzana Žunec |
| author_sort | Vilena Kašuba |
| collection | DOAJ |
| description | To contribute new information to the pyrethroid pesticide α-cypermethrin toxicity profile, we evaluated its effects after oral administration to Wistar rats at daily doses of 2.186, 0.015, 0.157, and 0.786 mg/kg bw for 28 days. Evaluations were performed using markers of oxidative stress, cholinesterase (ChE) activities, and levels of primary DNA damage in plasma/whole blood and liver, kidney, and brain tissue. Consecutive exposure to α-cypermethrin affected the kidney, liver, and brain weight of rats. A significant increase in concentration of the thiobarbituric acid reactive species was observed in the brain, accompanied by a significant increase in glutathione peroxidase (GPx) activity. An increase in GPx activity was also observed in the liver of all α-cypermethrin-treated groups, while GPx activity in the blood was significantly lower than in controls. A decrease in ChE activities was observed in the kidney and liver. Treatment with α-cypermethrin induced DNA damage in the studied cell types at almost all of the applied doses, indicating the highest susceptibility in the brain. The present study showed that, even at very low doses, exposure to α-cypermethrin exerts genotoxic effects and sets in motion the antioxidative mechanisms of cell defense, indicating the potential hazards posed by this insecticide. |
| first_indexed | 2024-03-09T15:47:27Z |
| format | Article |
| id | doaj.art-2887624df5e1443f8864e15215ae3e55 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-09T15:47:27Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-2887624df5e1443f8864e15215ae3e552023-11-24T18:24:38ZengMDPI AGToxics2305-63042022-11-01101271710.3390/toxics10120717Evaluation of Toxic Effects Induced by Sub-Acute Exposure to Low Doses of α-Cypermethrin in Adult Male RatsVilena Kašuba0Blanka Tariba Lovaković1Ana Lucić Vrdoljak2Anja Katić3Nevenka Kopjar4Vedran Micek5Mirta Milić6Alica Pizent7Davor Želježić8Suzana Žunec9Institute for Medical Research and Occupational Health, 10000 Zagreb, CroatiaInstitute for Medical Research and Occupational Health, 10000 Zagreb, CroatiaInstitute for Medical Research and Occupational Health, 10000 Zagreb, CroatiaInstitute for Medical Research and Occupational Health, 10000 Zagreb, CroatiaInstitute for Medical Research and Occupational Health, 10000 Zagreb, CroatiaInstitute for Medical Research and Occupational Health, 10000 Zagreb, CroatiaInstitute for Medical Research and Occupational Health, 10000 Zagreb, CroatiaInstitute for Medical Research and Occupational Health, 10000 Zagreb, CroatiaInstitute for Medical Research and Occupational Health, 10000 Zagreb, CroatiaInstitute for Medical Research and Occupational Health, 10000 Zagreb, CroatiaTo contribute new information to the pyrethroid pesticide α-cypermethrin toxicity profile, we evaluated its effects after oral administration to Wistar rats at daily doses of 2.186, 0.015, 0.157, and 0.786 mg/kg bw for 28 days. Evaluations were performed using markers of oxidative stress, cholinesterase (ChE) activities, and levels of primary DNA damage in plasma/whole blood and liver, kidney, and brain tissue. Consecutive exposure to α-cypermethrin affected the kidney, liver, and brain weight of rats. A significant increase in concentration of the thiobarbituric acid reactive species was observed in the brain, accompanied by a significant increase in glutathione peroxidase (GPx) activity. An increase in GPx activity was also observed in the liver of all α-cypermethrin-treated groups, while GPx activity in the blood was significantly lower than in controls. A decrease in ChE activities was observed in the kidney and liver. Treatment with α-cypermethrin induced DNA damage in the studied cell types at almost all of the applied doses, indicating the highest susceptibility in the brain. The present study showed that, even at very low doses, exposure to α-cypermethrin exerts genotoxic effects and sets in motion the antioxidative mechanisms of cell defense, indicating the potential hazards posed by this insecticide.https://www.mdpi.com/2305-6304/10/12/717cholinesterasegenotoxicityoxidative damagesubacute exposuresynthetic pyrethroids |
| spellingShingle | Vilena Kašuba Blanka Tariba Lovaković Ana Lucić Vrdoljak Anja Katić Nevenka Kopjar Vedran Micek Mirta Milić Alica Pizent Davor Želježić Suzana Žunec Evaluation of Toxic Effects Induced by Sub-Acute Exposure to Low Doses of α-Cypermethrin in Adult Male Rats Toxics cholinesterase genotoxicity oxidative damage subacute exposure synthetic pyrethroids |
| title | Evaluation of Toxic Effects Induced by Sub-Acute Exposure to Low Doses of α-Cypermethrin in Adult Male Rats |
| title_full | Evaluation of Toxic Effects Induced by Sub-Acute Exposure to Low Doses of α-Cypermethrin in Adult Male Rats |
| title_fullStr | Evaluation of Toxic Effects Induced by Sub-Acute Exposure to Low Doses of α-Cypermethrin in Adult Male Rats |
| title_full_unstemmed | Evaluation of Toxic Effects Induced by Sub-Acute Exposure to Low Doses of α-Cypermethrin in Adult Male Rats |
| title_short | Evaluation of Toxic Effects Induced by Sub-Acute Exposure to Low Doses of α-Cypermethrin in Adult Male Rats |
| title_sort | evaluation of toxic effects induced by sub acute exposure to low doses of α cypermethrin in adult male rats |
| topic | cholinesterase genotoxicity oxidative damage subacute exposure synthetic pyrethroids |
| url | https://www.mdpi.com/2305-6304/10/12/717 |
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