Expression Profiling of Non-Aflatoxigenic Aspergillus parasiticus Mutants Obtained by 5-Azacytosine Treatment or Serial Mycelial Transfer
Aflatoxins are carcinogenic secondary metabolites produced by the fungi Aspergillus flavus and Aspergillus parasiticus. Previous studies found that repeated serial mycelial transfer or treatment of A. parasiticus with 5-azacytidine produced colonies with a fluffy phenotype and inability to produce a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-08-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/3/8/932/ |
| _version_ | 1798005594127335424 |
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| author | Jiujiang Yu Kenneth C. Ehrlich Deepak Bhatnagar Shubha P. Kale Jeffrey R. Wilkinson |
| author_facet | Jiujiang Yu Kenneth C. Ehrlich Deepak Bhatnagar Shubha P. Kale Jeffrey R. Wilkinson |
| author_sort | Jiujiang Yu |
| collection | DOAJ |
| description | Aflatoxins are carcinogenic secondary metabolites produced by the fungi Aspergillus flavus and Aspergillus parasiticus. Previous studies found that repeated serial mycelial transfer or treatment of A. parasiticus with 5-azacytidine produced colonies with a fluffy phenotype and inability to produce aflatoxins. To understand how these treatments affect expression of genes involved in aflatoxin production and development, we carried out expressed sequence tag (EST)-based microarray assays to identify genes in treated clones that are differentially expressed compared to the wild-type. Expression of 183 genes was significantly dysregulated. Of these, 38 had at least two-fold or lower expression compared to the untreated control and only two had two-fold or higher expression. The most frequent change was downregulation of genes predicted to encode membrane-bound proteins. Based on this result we hypothesize that the treatments cause changes in the structure of cellular and organelle membranes that prevent normal development and aflatoxin biosynthesis. |
| first_indexed | 2024-04-11T12:42:52Z |
| format | Article |
| id | doaj.art-288ab826a7f3438b852ca74e3aea4848 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T12:42:52Z |
| publishDate | 2011-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-288ab826a7f3438b852ca74e3aea48482022-12-22T04:23:27ZengMDPI AGToxins2072-66512011-08-013893294810.3390/toxins3080932Expression Profiling of Non-Aflatoxigenic Aspergillus parasiticus Mutants Obtained by 5-Azacytosine Treatment or Serial Mycelial TransferJiujiang YuKenneth C. EhrlichDeepak BhatnagarShubha P. KaleJeffrey R. WilkinsonAflatoxins are carcinogenic secondary metabolites produced by the fungi Aspergillus flavus and Aspergillus parasiticus. Previous studies found that repeated serial mycelial transfer or treatment of A. parasiticus with 5-azacytidine produced colonies with a fluffy phenotype and inability to produce aflatoxins. To understand how these treatments affect expression of genes involved in aflatoxin production and development, we carried out expressed sequence tag (EST)-based microarray assays to identify genes in treated clones that are differentially expressed compared to the wild-type. Expression of 183 genes was significantly dysregulated. Of these, 38 had at least two-fold or lower expression compared to the untreated control and only two had two-fold or higher expression. The most frequent change was downregulation of genes predicted to encode membrane-bound proteins. Based on this result we hypothesize that the treatments cause changes in the structure of cellular and organelle membranes that prevent normal development and aflatoxin biosynthesis.http://www.mdpi.com/2072-6651/3/8/932/aflatoxinmicroarray5-azacytidineAspergillus parasiticussecondary metabolismfluffy phenotype |
| spellingShingle | Jiujiang Yu Kenneth C. Ehrlich Deepak Bhatnagar Shubha P. Kale Jeffrey R. Wilkinson Expression Profiling of Non-Aflatoxigenic Aspergillus parasiticus Mutants Obtained by 5-Azacytosine Treatment or Serial Mycelial Transfer Toxins aflatoxin microarray 5-azacytidine Aspergillus parasiticus secondary metabolism fluffy phenotype |
| title | Expression Profiling of Non-Aflatoxigenic Aspergillus parasiticus Mutants Obtained by 5-Azacytosine Treatment or Serial Mycelial Transfer |
| title_full | Expression Profiling of Non-Aflatoxigenic Aspergillus parasiticus Mutants Obtained by 5-Azacytosine Treatment or Serial Mycelial Transfer |
| title_fullStr | Expression Profiling of Non-Aflatoxigenic Aspergillus parasiticus Mutants Obtained by 5-Azacytosine Treatment or Serial Mycelial Transfer |
| title_full_unstemmed | Expression Profiling of Non-Aflatoxigenic Aspergillus parasiticus Mutants Obtained by 5-Azacytosine Treatment or Serial Mycelial Transfer |
| title_short | Expression Profiling of Non-Aflatoxigenic Aspergillus parasiticus Mutants Obtained by 5-Azacytosine Treatment or Serial Mycelial Transfer |
| title_sort | expression profiling of non aflatoxigenic aspergillus parasiticus mutants obtained by 5 azacytosine treatment or serial mycelial transfer |
| topic | aflatoxin microarray 5-azacytidine Aspergillus parasiticus secondary metabolism fluffy phenotype |
| url | http://www.mdpi.com/2072-6651/3/8/932/ |
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