Toxoplasmocidal and Cytotoxic Activities Guided Isolation and Characterization of an Undescribed Bioflavonoid-di-<i>C</i>-glucoside from <i>Cycas rumphii</i> Miq. Cultivated in Egypt
Toxoplasmosis and cancer are serious worldwide diseases, and the available drugs cause serious side effects. Investigation for new alternative therapies from natural sources is now an increasing concern. Herein, we carried out, for the first time, an in vitro screening of <i>Cycas rumphii</...
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MDPI AG
2022-10-01
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| author | Hosam M. El-Seadawy Kamilia A. Abo El-Seoud Mona El-Aasr Haytham O. Tawfik Amany E. Ragab |
| author_facet | Hosam M. El-Seadawy Kamilia A. Abo El-Seoud Mona El-Aasr Haytham O. Tawfik Amany E. Ragab |
| author_sort | Hosam M. El-Seadawy |
| collection | DOAJ |
| description | Toxoplasmosis and cancer are serious worldwide diseases, and the available drugs cause serious side effects. Investigation for new alternative therapies from natural sources is now an increasing concern. Herein, we carried out, for the first time, an in vitro screening of <i>Cycas rumphii</i> Miq. leaves for toxoplasmocidal effect, using <i>Viruluent RH Toxoplasma gondii,</i> and cytotoxic activity against HEPG-2, HCT-116 and HELA cancer cell lines using MTT assay. Among the tested extracts, the ethyl acetate fraction was the most effective against <i>T. gondii</i>, with an EC<sub>50</sub> of 3.51 ± 0.2 µg/mL compared to cotrimoxazole (4.18 ± 0.01 µg/mL) and was the most potent against the tested cell lines, especially HEPG-2, with an IC<sub>50</sub> of 6.98 ± 0.5 µg/mL compared to doxorubicin (4.50 ± 0.2 µg/mL). Seven compounds were isolated from the ethyl acetate fraction by extensive chromatographic techniques and fully elucidated using different spectroscopies. Compound (<b>7</b>) is an undescribed 4′, 4′′′ biapigenin di-C-glucoside, which showed a strong cytotoxic activity. Four known biflavonoids (<b>1</b>, <b>2</b>, <b>4</b> and <b>5</b>) in addition to a phenolic acid ester (<b>3</b>) and a flavonoid glycoside (<b>6</b>) were also isolated. Compounds (<b>1</b>, <b>3</b> and <b>6</b>) were reported for the first time from <i>C. rumphii</i>. |
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| language | English |
| last_indexed | 2024-03-09T18:43:48Z |
| publishDate | 2022-10-01 |
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| series | Plants |
| spelling | doaj.art-288bc777d24949c2b2193908e25abad22023-11-24T06:24:21ZengMDPI AGPlants2223-77472022-10-011121286710.3390/plants11212867Toxoplasmocidal and Cytotoxic Activities Guided Isolation and Characterization of an Undescribed Bioflavonoid-di-<i>C</i>-glucoside from <i>Cycas rumphii</i> Miq. Cultivated in EgyptHosam M. El-Seadawy0Kamilia A. Abo El-Seoud1Mona El-Aasr2Haytham O. Tawfik3Amany E. Ragab4Department of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta 31527, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta 31527, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta 31527, EgyptDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Tanta University, Tanta 31527, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta 31527, EgyptToxoplasmosis and cancer are serious worldwide diseases, and the available drugs cause serious side effects. Investigation for new alternative therapies from natural sources is now an increasing concern. Herein, we carried out, for the first time, an in vitro screening of <i>Cycas rumphii</i> Miq. leaves for toxoplasmocidal effect, using <i>Viruluent RH Toxoplasma gondii,</i> and cytotoxic activity against HEPG-2, HCT-116 and HELA cancer cell lines using MTT assay. Among the tested extracts, the ethyl acetate fraction was the most effective against <i>T. gondii</i>, with an EC<sub>50</sub> of 3.51 ± 0.2 µg/mL compared to cotrimoxazole (4.18 ± 0.01 µg/mL) and was the most potent against the tested cell lines, especially HEPG-2, with an IC<sub>50</sub> of 6.98 ± 0.5 µg/mL compared to doxorubicin (4.50 ± 0.2 µg/mL). Seven compounds were isolated from the ethyl acetate fraction by extensive chromatographic techniques and fully elucidated using different spectroscopies. Compound (<b>7</b>) is an undescribed 4′, 4′′′ biapigenin di-C-glucoside, which showed a strong cytotoxic activity. Four known biflavonoids (<b>1</b>, <b>2</b>, <b>4</b> and <b>5</b>) in addition to a phenolic acid ester (<b>3</b>) and a flavonoid glycoside (<b>6</b>) were also isolated. Compounds (<b>1</b>, <b>3</b> and <b>6</b>) were reported for the first time from <i>C. rumphii</i>.https://www.mdpi.com/2223-7747/11/21/2867<i>Cycas rumphii</i>Cycadaceaetoxoplasmosiscytotoxicityphenolic acidbiflavonoids |
| spellingShingle | Hosam M. El-Seadawy Kamilia A. Abo El-Seoud Mona El-Aasr Haytham O. Tawfik Amany E. Ragab Toxoplasmocidal and Cytotoxic Activities Guided Isolation and Characterization of an Undescribed Bioflavonoid-di-<i>C</i>-glucoside from <i>Cycas rumphii</i> Miq. Cultivated in Egypt Plants <i>Cycas rumphii</i> Cycadaceae toxoplasmosis cytotoxicity phenolic acid biflavonoids |
| title | Toxoplasmocidal and Cytotoxic Activities Guided Isolation and Characterization of an Undescribed Bioflavonoid-di-<i>C</i>-glucoside from <i>Cycas rumphii</i> Miq. Cultivated in Egypt |
| title_full | Toxoplasmocidal and Cytotoxic Activities Guided Isolation and Characterization of an Undescribed Bioflavonoid-di-<i>C</i>-glucoside from <i>Cycas rumphii</i> Miq. Cultivated in Egypt |
| title_fullStr | Toxoplasmocidal and Cytotoxic Activities Guided Isolation and Characterization of an Undescribed Bioflavonoid-di-<i>C</i>-glucoside from <i>Cycas rumphii</i> Miq. Cultivated in Egypt |
| title_full_unstemmed | Toxoplasmocidal and Cytotoxic Activities Guided Isolation and Characterization of an Undescribed Bioflavonoid-di-<i>C</i>-glucoside from <i>Cycas rumphii</i> Miq. Cultivated in Egypt |
| title_short | Toxoplasmocidal and Cytotoxic Activities Guided Isolation and Characterization of an Undescribed Bioflavonoid-di-<i>C</i>-glucoside from <i>Cycas rumphii</i> Miq. Cultivated in Egypt |
| title_sort | toxoplasmocidal and cytotoxic activities guided isolation and characterization of an undescribed bioflavonoid di i c i glucoside from i cycas rumphii i miq cultivated in egypt |
| topic | <i>Cycas rumphii</i> Cycadaceae toxoplasmosis cytotoxicity phenolic acid biflavonoids |
| url | https://www.mdpi.com/2223-7747/11/21/2867 |
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