Impact of <i>IsaA</i> Gene Disruption: Decreasing Staphylococcal Biofilm and Alteration of Transcriptomic and Proteomic Profiles
<i>Staphylococcus aureus</i> expresses diverse proteins at different stages of growth. The immunodominant staphylococcal antigen A (IsaA) is one of the proteins that is constitutively produced by <i>S. aureus</i> during colonisation and infection. SACOL2584 (or <i>isaA&...
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MDPI AG
2022-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/6/1119 |
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| author | Pei Yee Ma Chun Wie Chong Leslie Thian Lung Than Anita Binti Sulong Ket Li Ho Vasantha Kumari Neela Zamberi Sekawi Yun Khoon Liew |
| author_facet | Pei Yee Ma Chun Wie Chong Leslie Thian Lung Than Anita Binti Sulong Ket Li Ho Vasantha Kumari Neela Zamberi Sekawi Yun Khoon Liew |
| author_sort | Pei Yee Ma |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> expresses diverse proteins at different stages of growth. The immunodominant staphylococcal antigen A (IsaA) is one of the proteins that is constitutively produced by <i>S. aureus</i> during colonisation and infection. SACOL2584 (or <i>isaA</i>) is the gene that encodes this protein. It has been suggested that IsaA can hydrolyse cell walls, and there is still need to study <i>isaA</i> gene disruption to analyse its impact on staphylococcal phenotypes and on alteration to its transcription and protein profiles. In the present study, the growth curve in RPMI medium (which mimics human plasma), autolytic activity, cell wall morphology, fibronectin and fibrinogen adhesion and biofilm formation of <i>S. aureus</i> SH1000 (wildtype) was compared to that of <i>S. aureus</i> MS001 (<i>isaA</i> mutant). RNA sequencing and liquid chromatography–tandem mass spectrometry were carried out on samples of both <i>S. aureus</i> strains taken during the exponential growth phase, followed by bioinformatics analysis. Disruption of <i>isaA</i> had no obvious effect on the growth curve and autolysis ability or thickness of cell walls, but this study revealed significant strength of fibronectin adherence in <i>S. aureus</i> MS001. In particular, the <i>isaA</i> mutant formed less biofilm than <i>S. aureus</i> SH1000. In addition, proteomics and transcriptomics showed that the adhesin/biofilm-related genes and hemolysin genes, such as <i>sasF</i>, <i>sarX</i> and <i>hlgC</i>, were consistently downregulated with <i>isaA</i> gene disruption. The majority of the upregulated genes or proteins in <i>S. aureus</i> MS001 were <i>pur</i> genes. Taken together, this study provides insight into how <i>isaA</i> disruption changes the expression of other genes and has implications regarding biofilm formation and biological processes. |
| first_indexed | 2024-03-09T22:59:48Z |
| format | Article |
| id | doaj.art-288e6f9d1a604223b139ce1764810fb0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T22:59:48Z |
| publishDate | 2022-05-01 |
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| series | Microorganisms |
| spelling | doaj.art-288e6f9d1a604223b139ce1764810fb02023-11-23T18:03:18ZengMDPI AGMicroorganisms2076-26072022-05-01106111910.3390/microorganisms10061119Impact of <i>IsaA</i> Gene Disruption: Decreasing Staphylococcal Biofilm and Alteration of Transcriptomic and Proteomic ProfilesPei Yee Ma0Chun Wie Chong1Leslie Thian Lung Than2Anita Binti Sulong3Ket Li Ho4Vasantha Kumari Neela5Zamberi Sekawi6Yun Khoon Liew7School of Postgraduate Studies, International Medical University, Kuala Lumpur 57000, MalaysiaSchool of Pharmacy, Monash University Malaysia, Subang Jaya 47500, MalaysiaDepartment of Medical Microbiology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang 43400, MalaysiaDepartment of Medical Microbiology and Immunology, Pusat Perubatan UKM, Kuala Lumpur 56000, MalaysiaDepartment of Life Sciences, School of Pharmacy, International Medical University, Kuala Lumpur 57000, MalaysiaDepartment of Medical Microbiology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang 43400, MalaysiaDepartment of Medical Microbiology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang 43400, MalaysiaDepartment of Life Sciences, School of Pharmacy, International Medical University, Kuala Lumpur 57000, Malaysia<i>Staphylococcus aureus</i> expresses diverse proteins at different stages of growth. The immunodominant staphylococcal antigen A (IsaA) is one of the proteins that is constitutively produced by <i>S. aureus</i> during colonisation and infection. SACOL2584 (or <i>isaA</i>) is the gene that encodes this protein. It has been suggested that IsaA can hydrolyse cell walls, and there is still need to study <i>isaA</i> gene disruption to analyse its impact on staphylococcal phenotypes and on alteration to its transcription and protein profiles. In the present study, the growth curve in RPMI medium (which mimics human plasma), autolytic activity, cell wall morphology, fibronectin and fibrinogen adhesion and biofilm formation of <i>S. aureus</i> SH1000 (wildtype) was compared to that of <i>S. aureus</i> MS001 (<i>isaA</i> mutant). RNA sequencing and liquid chromatography–tandem mass spectrometry were carried out on samples of both <i>S. aureus</i> strains taken during the exponential growth phase, followed by bioinformatics analysis. Disruption of <i>isaA</i> had no obvious effect on the growth curve and autolysis ability or thickness of cell walls, but this study revealed significant strength of fibronectin adherence in <i>S. aureus</i> MS001. In particular, the <i>isaA</i> mutant formed less biofilm than <i>S. aureus</i> SH1000. In addition, proteomics and transcriptomics showed that the adhesin/biofilm-related genes and hemolysin genes, such as <i>sasF</i>, <i>sarX</i> and <i>hlgC</i>, were consistently downregulated with <i>isaA</i> gene disruption. The majority of the upregulated genes or proteins in <i>S. aureus</i> MS001 were <i>pur</i> genes. Taken together, this study provides insight into how <i>isaA</i> disruption changes the expression of other genes and has implications regarding biofilm formation and biological processes.https://www.mdpi.com/2076-2607/10/6/1119<i>Staphylococcus aureus</i>IsaAgene disruptionphenotypeproteomictranscriptomic |
| spellingShingle | Pei Yee Ma Chun Wie Chong Leslie Thian Lung Than Anita Binti Sulong Ket Li Ho Vasantha Kumari Neela Zamberi Sekawi Yun Khoon Liew Impact of <i>IsaA</i> Gene Disruption: Decreasing Staphylococcal Biofilm and Alteration of Transcriptomic and Proteomic Profiles Microorganisms <i>Staphylococcus aureus</i> IsaA gene disruption phenotype proteomic transcriptomic |
| title | Impact of <i>IsaA</i> Gene Disruption: Decreasing Staphylococcal Biofilm and Alteration of Transcriptomic and Proteomic Profiles |
| title_full | Impact of <i>IsaA</i> Gene Disruption: Decreasing Staphylococcal Biofilm and Alteration of Transcriptomic and Proteomic Profiles |
| title_fullStr | Impact of <i>IsaA</i> Gene Disruption: Decreasing Staphylococcal Biofilm and Alteration of Transcriptomic and Proteomic Profiles |
| title_full_unstemmed | Impact of <i>IsaA</i> Gene Disruption: Decreasing Staphylococcal Biofilm and Alteration of Transcriptomic and Proteomic Profiles |
| title_short | Impact of <i>IsaA</i> Gene Disruption: Decreasing Staphylococcal Biofilm and Alteration of Transcriptomic and Proteomic Profiles |
| title_sort | impact of i isaa i gene disruption decreasing staphylococcal biofilm and alteration of transcriptomic and proteomic profiles |
| topic | <i>Staphylococcus aureus</i> IsaA gene disruption phenotype proteomic transcriptomic |
| url | https://www.mdpi.com/2076-2607/10/6/1119 |
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