Molecular Detection of Avian Infectious Bronchitis Virus Serotypes from Clinically Suspected Broiler Chicken Flocks in Fars Province of Iran

This study was conducted to detect the serotypes Arkansas, Connecticut, Beaudette and Florida in broiler chicken farms in Fars province of Iran. The samples were obtained from 32 broiler chicken farms experiencing respiratory signs with high mortality and clinically diagnosed as avian infectious bro...

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Bibliographic Details
Main Author: Seyedeh Leila Poorbaghi1, Ali Mohammadi2* and Keramat Asasi3
Format: Article
Language:English
Published: University of Agriculture, Faisalabad 2012-01-01
Series:Pakistan Veterinary Journal
Subjects:
Online Access:http://pvj.com.pk/pdf-files/32_1/93-96.pdf
Description
Summary:This study was conducted to detect the serotypes Arkansas, Connecticut, Beaudette and Florida in broiler chicken farms in Fars province of Iran. The samples were obtained from 32 broiler chicken farms experiencing respiratory signs with high mortality and clinically diagnosed as avian infectious bronchitis. Total RNAs were extracted from the scrapped mucosa of tracheas and RT-PCR tests were performed using different types of primers including Del1 (F, R), (XCE-1, 2), (XCE-3  BCE-1), (XCE-3  MCE-1), S1 OLIGO (F, R) and S1 OLIGO NEW (F, R). Infectious bronchitis virus was detected in 72% poultry farms. The serotypes 4/91 and Massachusetts were detected in 47.9 and 21.8% farms, respectively, but the serotypes in 7 farms remained unclear. The results showed that there is no evidence for the presence of serotypes Arkansas, Connecticut, Beaudette and Florida in broiler chicken farms in Fars province of Iran.
ISSN:0253-8318