Molecular Detection of Avian Infectious Bronchitis Virus Serotypes from Clinically Suspected Broiler Chicken Flocks in Fars Province of Iran
This study was conducted to detect the serotypes Arkansas, Connecticut, Beaudette and Florida in broiler chicken farms in Fars province of Iran. The samples were obtained from 32 broiler chicken farms experiencing respiratory signs with high mortality and clinically diagnosed as avian infectious bro...
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| Format: | Article |
| Language: | English |
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University of Agriculture, Faisalabad
2012-01-01
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| Series: | Pakistan Veterinary Journal |
| Subjects: | |
| Online Access: | http://pvj.com.pk/pdf-files/32_1/93-96.pdf |
| _version_ | 1818434682771996672 |
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| author | Seyedeh Leila Poorbaghi1, Ali Mohammadi2* and Keramat Asasi3 |
| author_facet | Seyedeh Leila Poorbaghi1, Ali Mohammadi2* and Keramat Asasi3 |
| author_sort | Seyedeh Leila Poorbaghi1, Ali Mohammadi2* and Keramat Asasi3 |
| collection | DOAJ |
| description | This study was conducted to detect the serotypes Arkansas, Connecticut, Beaudette and Florida in broiler chicken farms in Fars province of Iran. The samples were obtained from 32 broiler chicken farms experiencing respiratory signs with high mortality and clinically diagnosed as avian infectious bronchitis. Total RNAs were extracted from the scrapped mucosa of tracheas and RT-PCR tests were performed using different types of primers including Del1 (F, R), (XCE-1, 2), (XCE-3 BCE-1), (XCE-3 MCE-1), S1 OLIGO (F, R) and S1 OLIGO NEW (F, R). Infectious bronchitis virus was detected in 72% poultry farms. The serotypes 4/91 and Massachusetts were detected in 47.9 and 21.8% farms, respectively, but the serotypes in 7 farms remained unclear. The results showed that there is no evidence for the presence of serotypes Arkansas, Connecticut, Beaudette and Florida in broiler chicken farms in Fars province of Iran. |
| first_indexed | 2024-12-14T16:40:53Z |
| format | Article |
| id | doaj.art-288eb2bfe2c94a8a9e2e1c93668423df |
| institution | Directory Open Access Journal |
| issn | 0253-8318 |
| language | English |
| last_indexed | 2024-12-14T16:40:53Z |
| publishDate | 2012-01-01 |
| publisher | University of Agriculture, Faisalabad |
| record_format | Article |
| series | Pakistan Veterinary Journal |
| spelling | doaj.art-288eb2bfe2c94a8a9e2e1c93668423df2022-12-21T22:54:20ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182012-01-013219396Molecular Detection of Avian Infectious Bronchitis Virus Serotypes from Clinically Suspected Broiler Chicken Flocks in Fars Province of IranSeyedeh Leila Poorbaghi1, Ali Mohammadi2* and Keramat Asasi3This study was conducted to detect the serotypes Arkansas, Connecticut, Beaudette and Florida in broiler chicken farms in Fars province of Iran. The samples were obtained from 32 broiler chicken farms experiencing respiratory signs with high mortality and clinically diagnosed as avian infectious bronchitis. Total RNAs were extracted from the scrapped mucosa of tracheas and RT-PCR tests were performed using different types of primers including Del1 (F, R), (XCE-1, 2), (XCE-3 BCE-1), (XCE-3 MCE-1), S1 OLIGO (F, R) and S1 OLIGO NEW (F, R). Infectious bronchitis virus was detected in 72% poultry farms. The serotypes 4/91 and Massachusetts were detected in 47.9 and 21.8% farms, respectively, but the serotypes in 7 farms remained unclear. The results showed that there is no evidence for the presence of serotypes Arkansas, Connecticut, Beaudette and Florida in broiler chicken farms in Fars province of Iran.http://pvj.com.pk/pdf-files/32_1/93-96.pdfBroiler chickenInfectious bronchitis virusIranMassachusettsRT-PCRSerotype 4/91 |
| spellingShingle | Seyedeh Leila Poorbaghi1, Ali Mohammadi2* and Keramat Asasi3 Molecular Detection of Avian Infectious Bronchitis Virus Serotypes from Clinically Suspected Broiler Chicken Flocks in Fars Province of Iran Pakistan Veterinary Journal Broiler chicken Infectious bronchitis virus Iran Massachusetts RT-PCR Serotype 4/91 |
| title | Molecular Detection of Avian Infectious Bronchitis Virus Serotypes from Clinically Suspected Broiler Chicken Flocks in Fars Province of Iran |
| title_full | Molecular Detection of Avian Infectious Bronchitis Virus Serotypes from Clinically Suspected Broiler Chicken Flocks in Fars Province of Iran |
| title_fullStr | Molecular Detection of Avian Infectious Bronchitis Virus Serotypes from Clinically Suspected Broiler Chicken Flocks in Fars Province of Iran |
| title_full_unstemmed | Molecular Detection of Avian Infectious Bronchitis Virus Serotypes from Clinically Suspected Broiler Chicken Flocks in Fars Province of Iran |
| title_short | Molecular Detection of Avian Infectious Bronchitis Virus Serotypes from Clinically Suspected Broiler Chicken Flocks in Fars Province of Iran |
| title_sort | molecular detection of avian infectious bronchitis virus serotypes from clinically suspected broiler chicken flocks in fars province of iran |
| topic | Broiler chicken Infectious bronchitis virus Iran Massachusetts RT-PCR Serotype 4/91 |
| url | http://pvj.com.pk/pdf-files/32_1/93-96.pdf |
| work_keys_str_mv | AT seyedehleilapoorbaghi1alimohammadi2andkeramatasasi3 moleculardetectionofavianinfectiousbronchitisvirusserotypesfromclinicallysuspectedbroilerchickenflocksinfarsprovinceofiran |