Stimulation of Tetrahydrobiopterin Synthesis by Basic Fibroblast Growth Factor in Vascular Endothelial Cells
The purpose of this study was to examine whether basic fibroblast growth factor (bFGF) stimulates tetrahydrobiopterin (BH4) synthesis in mouse brain microvascular endothelial cells. BH4 content was determined by oxidation under acidic conditions as biopterin and analysed with reversed-phase high Per...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2003-02-01
|
| Series: | Pteridines |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/pteridines.2003.14.1.9 |
| _version_ | 1819102152300167168 |
|---|---|
| author | Shimizu Shunichi Miyasaka Yoshiyuki Yamamoto Shinichiro Ishii Masakazu Kiuchi Yuji |
| author_facet | Shimizu Shunichi Miyasaka Yoshiyuki Yamamoto Shinichiro Ishii Masakazu Kiuchi Yuji |
| author_sort | Shimizu Shunichi |
| collection | DOAJ |
| description | The purpose of this study was to examine whether basic fibroblast growth factor (bFGF) stimulates tetrahydrobiopterin (BH4) synthesis in mouse brain microvascular endothelial cells. BH4 content was determined by oxidation under acidic conditions as biopterin and analysed with reversed-phase high Performance liquid chromatography. Measurement of the mRNA level of QTP-cyclohydrolase I (GTPCH), which is the rate-limiting enzyme of the de novo pathway of BH4 synthesis. The addition of bFGF to endothelial cells increased the BH4 content and GTPCH mRNA levels in an incubation period- and a concentration-dependent manner. 2,4-Diamino-6- hydroxypyrimidine, an inhibitor of GTPCH, strongly reduced the bFGF-induced increase in BH4 content. These findings suggest that bFGF stimulates BH4 synthesis via a de novo pathway with the induction of GTPCH. |
| first_indexed | 2024-12-22T01:30:01Z |
| format | Article |
| id | doaj.art-288fe73ba41840a1803d3a2a639a3f4f |
| institution | Directory Open Access Journal |
| issn | 0933-4807 2195-4720 |
| language | English |
| last_indexed | 2024-12-22T01:30:01Z |
| publishDate | 2003-02-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Pteridines |
| spelling | doaj.art-288fe73ba41840a1803d3a2a639a3f4f2022-12-21T18:43:31ZengDe GruyterPteridines0933-48072195-47202003-02-0114191210.1515/pteridines.2003.14.1.9Stimulation of Tetrahydrobiopterin Synthesis by Basic Fibroblast Growth Factor in Vascular Endothelial CellsShimizu Shunichi0Miyasaka Yoshiyuki1Yamamoto Shinichiro2Ishii Masakazu3Kiuchi Yuji4Department of Pathophysiology, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Pathophysiology, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Pathophysiology, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Pathophysiology, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Pathophysiology, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanThe purpose of this study was to examine whether basic fibroblast growth factor (bFGF) stimulates tetrahydrobiopterin (BH4) synthesis in mouse brain microvascular endothelial cells. BH4 content was determined by oxidation under acidic conditions as biopterin and analysed with reversed-phase high Performance liquid chromatography. Measurement of the mRNA level of QTP-cyclohydrolase I (GTPCH), which is the rate-limiting enzyme of the de novo pathway of BH4 synthesis. The addition of bFGF to endothelial cells increased the BH4 content and GTPCH mRNA levels in an incubation period- and a concentration-dependent manner. 2,4-Diamino-6- hydroxypyrimidine, an inhibitor of GTPCH, strongly reduced the bFGF-induced increase in BH4 content. These findings suggest that bFGF stimulates BH4 synthesis via a de novo pathway with the induction of GTPCH.https://doi.org/10.1515/pteridines.2003.14.1.9bfgftetrahydrobiopteringtp-cyclohydrolase ivascular endothelial cells |
| spellingShingle | Shimizu Shunichi Miyasaka Yoshiyuki Yamamoto Shinichiro Ishii Masakazu Kiuchi Yuji Stimulation of Tetrahydrobiopterin Synthesis by Basic Fibroblast Growth Factor in Vascular Endothelial Cells Pteridines bfgf tetrahydrobiopterin gtp-cyclohydrolase i vascular endothelial cells |
| title | Stimulation of Tetrahydrobiopterin Synthesis by Basic Fibroblast Growth Factor in Vascular Endothelial Cells |
| title_full | Stimulation of Tetrahydrobiopterin Synthesis by Basic Fibroblast Growth Factor in Vascular Endothelial Cells |
| title_fullStr | Stimulation of Tetrahydrobiopterin Synthesis by Basic Fibroblast Growth Factor in Vascular Endothelial Cells |
| title_full_unstemmed | Stimulation of Tetrahydrobiopterin Synthesis by Basic Fibroblast Growth Factor in Vascular Endothelial Cells |
| title_short | Stimulation of Tetrahydrobiopterin Synthesis by Basic Fibroblast Growth Factor in Vascular Endothelial Cells |
| title_sort | stimulation of tetrahydrobiopterin synthesis by basic fibroblast growth factor in vascular endothelial cells |
| topic | bfgf tetrahydrobiopterin gtp-cyclohydrolase i vascular endothelial cells |
| url | https://doi.org/10.1515/pteridines.2003.14.1.9 |
| work_keys_str_mv | AT shimizushunichi stimulationoftetrahydrobiopterinsynthesisbybasicfibroblastgrowthfactorinvascularendothelialcells AT miyasakayoshiyuki stimulationoftetrahydrobiopterinsynthesisbybasicfibroblastgrowthfactorinvascularendothelialcells AT yamamotoshinichiro stimulationoftetrahydrobiopterinsynthesisbybasicfibroblastgrowthfactorinvascularendothelialcells AT ishiimasakazu stimulationoftetrahydrobiopterinsynthesisbybasicfibroblastgrowthfactorinvascularendothelialcells AT kiuchiyuji stimulationoftetrahydrobiopterinsynthesisbybasicfibroblastgrowthfactorinvascularendothelialcells |