Simple and Reliable Determination of the Histamine Content of Selected Greek Vegetables and Related Products in the Frame of “Low Histamine Diet”

The determination of histamine in Greek foods that should potentially be avoided during a “low histamine diet” is reported herein. Cation exchange chromatography combined to selective post column derivatization proved to be an excellent tool for this type of analysis as well, offering accurate resul...

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Main Authors: Apostolia Tsiasioti, Paraskevas D. Tzanavaras
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/11/20/3234
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author Apostolia Tsiasioti
Paraskevas D. Tzanavaras
author_facet Apostolia Tsiasioti
Paraskevas D. Tzanavaras
author_sort Apostolia Tsiasioti
collection DOAJ
description The determination of histamine in Greek foods that should potentially be avoided during a “low histamine diet” is reported herein. Cation exchange chromatography combined to selective post column derivatization proved to be an excellent tool for this type of analysis as well, offering accurate results following minimal sample preparation. Tomato-, eggplant- and spinach-related products have been successfully analyzed and were all found to contain histamine. Higher amounts were quantified in eggplants, eggplant salads and spinach in the range of 15.4–34.2 mg kg<sup>−1</sup> and lower in fresh tomatoes and related products (0.8–10.6 mg kg<sup>−1</sup>). The method is capable of determining as low as 0.5 mg kg<sup>−1</sup> histamine without matrix effects, with percent recoveries ranging between 87 and 112% (tomatoes and related products), 95 and 119% (eggplants and related products) and 90 and 106% (fresh and frozen spinach).
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spelling doaj.art-289600d2c9054794bbcee8437bffbda22023-11-24T00:07:54ZengMDPI AGFoods2304-81582022-10-011120323410.3390/foods11203234Simple and Reliable Determination of the Histamine Content of Selected Greek Vegetables and Related Products in the Frame of “Low Histamine Diet”Apostolia Tsiasioti0Paraskevas D. Tzanavaras1Laboratory of Analytical Chemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceLaboratory of Analytical Chemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceThe determination of histamine in Greek foods that should potentially be avoided during a “low histamine diet” is reported herein. Cation exchange chromatography combined to selective post column derivatization proved to be an excellent tool for this type of analysis as well, offering accurate results following minimal sample preparation. Tomato-, eggplant- and spinach-related products have been successfully analyzed and were all found to contain histamine. Higher amounts were quantified in eggplants, eggplant salads and spinach in the range of 15.4–34.2 mg kg<sup>−1</sup> and lower in fresh tomatoes and related products (0.8–10.6 mg kg<sup>−1</sup>). The method is capable of determining as low as 0.5 mg kg<sup>−1</sup> histamine without matrix effects, with percent recoveries ranging between 87 and 112% (tomatoes and related products), 95 and 119% (eggplants and related products) and 90 and 106% (fresh and frozen spinach).https://www.mdpi.com/2304-8158/11/20/3234histaminelow histamine dietvegetablesfood samplespost column derivatization
spellingShingle Apostolia Tsiasioti
Paraskevas D. Tzanavaras
Simple and Reliable Determination of the Histamine Content of Selected Greek Vegetables and Related Products in the Frame of “Low Histamine Diet”
Foods
histamine
low histamine diet
vegetables
food samples
post column derivatization
title Simple and Reliable Determination of the Histamine Content of Selected Greek Vegetables and Related Products in the Frame of “Low Histamine Diet”
title_full Simple and Reliable Determination of the Histamine Content of Selected Greek Vegetables and Related Products in the Frame of “Low Histamine Diet”
title_fullStr Simple and Reliable Determination of the Histamine Content of Selected Greek Vegetables and Related Products in the Frame of “Low Histamine Diet”
title_full_unstemmed Simple and Reliable Determination of the Histamine Content of Selected Greek Vegetables and Related Products in the Frame of “Low Histamine Diet”
title_short Simple and Reliable Determination of the Histamine Content of Selected Greek Vegetables and Related Products in the Frame of “Low Histamine Diet”
title_sort simple and reliable determination of the histamine content of selected greek vegetables and related products in the frame of low histamine diet
topic histamine
low histamine diet
vegetables
food samples
post column derivatization
url https://www.mdpi.com/2304-8158/11/20/3234
work_keys_str_mv AT apostoliatsiasioti simpleandreliabledeterminationofthehistaminecontentofselectedgreekvegetablesandrelatedproductsintheframeoflowhistaminediet
AT paraskevasdtzanavaras simpleandreliabledeterminationofthehistaminecontentofselectedgreekvegetablesandrelatedproductsintheframeoflowhistaminediet