Memory B-cells are enriched in the blood of patients with acute Buruli ulcer disease: a prospective observational study
Abstract Background Buruli ulcer disease (BUD) caused by Mycobacterium (M.) ulcerans is characterized by necrotic skin lesions. As for other mycobacterial infections, e.g., tuberculosis, the immune response is important for host protection. B-cells may play a role in antimycobacterial immunity but s...
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12879-023-08370-1 |
| _version_ | 1797801557654241280 |
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| author | Jonathan Kofi Adjei Wilfred Aniagyei Ernest Adankwah Julia Seyfarth Ertan Mayatepek Daniel Antwi Berko Nancy Ackam Max Efui Annani-Akollor Samuel Asamoah Sakyi Yaw Ampem Amoako Dorcas Owusu Marc Jacobsen Richard Odame Phillips |
| author_facet | Jonathan Kofi Adjei Wilfred Aniagyei Ernest Adankwah Julia Seyfarth Ertan Mayatepek Daniel Antwi Berko Nancy Ackam Max Efui Annani-Akollor Samuel Asamoah Sakyi Yaw Ampem Amoako Dorcas Owusu Marc Jacobsen Richard Odame Phillips |
| author_sort | Jonathan Kofi Adjei |
| collection | DOAJ |
| description | Abstract Background Buruli ulcer disease (BUD) caused by Mycobacterium (M.) ulcerans is characterized by necrotic skin lesions. As for other mycobacterial infections, e.g., tuberculosis, the immune response is important for host protection. B-cells may play a role in antimycobacterial immunity but studies characterizing the B-cell repertoire and memory generation in BUD and during the course of treatment are scarce. Methods We investigated the adaptive immune cell repertoire in children with BUD and healthy matched controls by flow cytometry. Analyses prior to treatment, also in a study group of patients with tuberculosis, as well as three time points during BUD treatment (i.e., week 8, 16, and 32) were performed. In addition, BUD disease severity as well as treatment response were analysed for association with B-cell repertoire differences. Results Children with BUD had comparable total B- and T-cell proportions but differed largely in B-cell subsets. Memory B-cell (B mem) proportions were higher in children with BUD whereas regulatory B-cell (B reg) proportions were lower as compared to healthy controls and tuberculosis patients. Lower naïve (B naïve) and higher transitional B-cell (B trans) proportions characterized children with BUD in comparison with tuberculosis patients. Under treatment, B mem proportions decreased significantly whereas proportions of B reg and B naive increased concomitantly in children with BUD. Also, we found significant correlation between lesion size and B mem as well as B reg. However, we did not detect associations between treatment efficacy and B-cell proportions. Conclusions These results suggest a role of B-cell subsets in the immune response against M. ulcerans. Furthermore, changes in B-cell subset proportions may be used as markers for treatment monitoring in BUD. |
| first_indexed | 2024-03-13T04:52:12Z |
| format | Article |
| id | doaj.art-28972d50e30647be8f63bde59f6d7be7 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-03-13T04:52:12Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-28972d50e30647be8f63bde59f6d7be72023-06-18T11:07:54ZengBMCBMC Infectious Diseases1471-23342023-06-012311910.1186/s12879-023-08370-1Memory B-cells are enriched in the blood of patients with acute Buruli ulcer disease: a prospective observational studyJonathan Kofi Adjei0Wilfred Aniagyei1Ernest Adankwah2Julia Seyfarth3Ertan Mayatepek4Daniel Antwi Berko5Nancy Ackam6Max Efui Annani-Akollor7Samuel Asamoah Sakyi8Yaw Ampem Amoako9Dorcas Owusu10Marc Jacobsen11Richard Odame Phillips12Kumasi Centre for Collaborative Research in Tropical MedicineKumasi Centre for Collaborative Research in Tropical MedicineKumasi Centre for Collaborative Research in Tropical MedicineDepartment of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, University Hospital Düsseldorf, Heinrich-Heine-UniversityDepartment of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, University Hospital Düsseldorf, Heinrich-Heine-UniversityKumasi Centre for Collaborative Research in Tropical MedicineKumasi Centre for Collaborative Research in Tropical MedicineDepartment of Molecular Medicine, School of Medicine and Dentistry, College of Health Sciences, Kwame Nkrumah University of Science and Technology (KNUST)Department of Molecular Medicine, School of Medicine and Dentistry, College of Health Sciences, Kwame Nkrumah University of Science and Technology (KNUST)Kumasi Centre for Collaborative Research in Tropical MedicineKumasi Centre for Collaborative Research in Tropical MedicineDepartment of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, University Hospital Düsseldorf, Heinrich-Heine-UniversityKumasi Centre for Collaborative Research in Tropical MedicineAbstract Background Buruli ulcer disease (BUD) caused by Mycobacterium (M.) ulcerans is characterized by necrotic skin lesions. As for other mycobacterial infections, e.g., tuberculosis, the immune response is important for host protection. B-cells may play a role in antimycobacterial immunity but studies characterizing the B-cell repertoire and memory generation in BUD and during the course of treatment are scarce. Methods We investigated the adaptive immune cell repertoire in children with BUD and healthy matched controls by flow cytometry. Analyses prior to treatment, also in a study group of patients with tuberculosis, as well as three time points during BUD treatment (i.e., week 8, 16, and 32) were performed. In addition, BUD disease severity as well as treatment response were analysed for association with B-cell repertoire differences. Results Children with BUD had comparable total B- and T-cell proportions but differed largely in B-cell subsets. Memory B-cell (B mem) proportions were higher in children with BUD whereas regulatory B-cell (B reg) proportions were lower as compared to healthy controls and tuberculosis patients. Lower naïve (B naïve) and higher transitional B-cell (B trans) proportions characterized children with BUD in comparison with tuberculosis patients. Under treatment, B mem proportions decreased significantly whereas proportions of B reg and B naive increased concomitantly in children with BUD. Also, we found significant correlation between lesion size and B mem as well as B reg. However, we did not detect associations between treatment efficacy and B-cell proportions. Conclusions These results suggest a role of B-cell subsets in the immune response against M. ulcerans. Furthermore, changes in B-cell subset proportions may be used as markers for treatment monitoring in BUD.https://doi.org/10.1186/s12879-023-08370-1B-cell subsetsBuruli ulcerMycobacterium ulceransBiomarker |
| spellingShingle | Jonathan Kofi Adjei Wilfred Aniagyei Ernest Adankwah Julia Seyfarth Ertan Mayatepek Daniel Antwi Berko Nancy Ackam Max Efui Annani-Akollor Samuel Asamoah Sakyi Yaw Ampem Amoako Dorcas Owusu Marc Jacobsen Richard Odame Phillips Memory B-cells are enriched in the blood of patients with acute Buruli ulcer disease: a prospective observational study BMC Infectious Diseases B-cell subsets Buruli ulcer Mycobacterium ulcerans Biomarker |
| title | Memory B-cells are enriched in the blood of patients with acute Buruli ulcer disease: a prospective observational study |
| title_full | Memory B-cells are enriched in the blood of patients with acute Buruli ulcer disease: a prospective observational study |
| title_fullStr | Memory B-cells are enriched in the blood of patients with acute Buruli ulcer disease: a prospective observational study |
| title_full_unstemmed | Memory B-cells are enriched in the blood of patients with acute Buruli ulcer disease: a prospective observational study |
| title_short | Memory B-cells are enriched in the blood of patients with acute Buruli ulcer disease: a prospective observational study |
| title_sort | memory b cells are enriched in the blood of patients with acute buruli ulcer disease a prospective observational study |
| topic | B-cell subsets Buruli ulcer Mycobacterium ulcerans Biomarker |
| url | https://doi.org/10.1186/s12879-023-08370-1 |
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