Transgenic tobacco cells: a permissive system for the assessment of resistance strategies against Tomato yellow leaf curl virus
Tomato yellow leaf curl virus (TYLCV) is a major threat to tomato production in the tropics and sub-tropics around the world. The application of genetic engineering and pathogen derived resistance mechanisms to obtain tomatoes that are resistant to this pathogen is considered a promising alternative...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elfos Scientiae
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| Series: | Biotecnología Aplicada |
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| Online Access: | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522009000200003&lng=en&tlng=en |
| _version_ | 1818451246813544448 |
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| author | Alejandro D Fuentes Pedro L Ramos Ana I Fernández Kenia Tiel Danay Callard Yadira Sánchez Merardo Pujol |
| author_facet | Alejandro D Fuentes Pedro L Ramos Ana I Fernández Kenia Tiel Danay Callard Yadira Sánchez Merardo Pujol |
| author_sort | Alejandro D Fuentes |
| collection | DOAJ |
| description | Tomato yellow leaf curl virus (TYLCV) is a major threat to tomato production in the tropics and sub-tropics around the world. The application of genetic engineering and pathogen derived resistance mechanisms to obtain tomatoes that are resistant to this pathogen is considered a promising alternative to the current protective practice against the virus. However, the development of transgenic tomato plants that are resistant to the virus is a resourceconsuming and time-consuming procedure, often with unpredictable efficiency, which hinders the evaluation of genetic designs. For this reason an assessment of the strategies against TYLCV replication preceding transgenic tomato development would ensure the protective potential of the candidate transgene. Attempting to circumvent this issue, the present study demonstrated the feasibility of using tobacco cell lines to study the consequences of c1 antisense expression on TYLCV replication. As a result, the transgenic tobacco cell lines were able to produce siRNA that is complementary to the c1 sequence and inhibited TYLCV multiplication, forecasting what would happen in transgenic plants harboring this antiviral strategy. |
| first_indexed | 2024-12-14T21:04:09Z |
| format | Article |
| id | doaj.art-289854795599411aa4efafd6fabfbfbb |
| institution | Directory Open Access Journal |
| issn | 1027-2852 |
| language | English |
| last_indexed | 2024-12-14T21:04:09Z |
| publisher | Elfos Scientiae |
| record_format | Article |
| series | Biotecnología Aplicada |
| spelling | doaj.art-289854795599411aa4efafd6fabfbfbb2022-12-21T22:47:31ZengElfos ScientiaeBiotecnología Aplicada1027-2852262122126S1027-28522009000200003Transgenic tobacco cells: a permissive system for the assessment of resistance strategies against Tomato yellow leaf curl virusAlejandro D Fuentes0Pedro L Ramos1Ana I Fernández2Kenia Tiel3Danay Callard4Yadira Sánchez5Merardo Pujol6International Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyTomato yellow leaf curl virus (TYLCV) is a major threat to tomato production in the tropics and sub-tropics around the world. The application of genetic engineering and pathogen derived resistance mechanisms to obtain tomatoes that are resistant to this pathogen is considered a promising alternative to the current protective practice against the virus. However, the development of transgenic tomato plants that are resistant to the virus is a resourceconsuming and time-consuming procedure, often with unpredictable efficiency, which hinders the evaluation of genetic designs. For this reason an assessment of the strategies against TYLCV replication preceding transgenic tomato development would ensure the protective potential of the candidate transgene. Attempting to circumvent this issue, the present study demonstrated the feasibility of using tobacco cell lines to study the consequences of c1 antisense expression on TYLCV replication. As a result, the transgenic tobacco cell lines were able to produce siRNA that is complementary to the c1 sequence and inhibited TYLCV multiplication, forecasting what would happen in transgenic plants harboring this antiviral strategy.http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522009000200003&lng=en&tlng=enbegomovirustyccutabacosilenciamiento génico post-transcripcionalresistencia derivada del patógenotomate |
| spellingShingle | Alejandro D Fuentes Pedro L Ramos Ana I Fernández Kenia Tiel Danay Callard Yadira Sánchez Merardo Pujol Transgenic tobacco cells: a permissive system for the assessment of resistance strategies against Tomato yellow leaf curl virus Biotecnología Aplicada begomovirus tyccu tabaco silenciamiento génico post-transcripcional resistencia derivada del patógeno tomate |
| title | Transgenic tobacco cells: a permissive system for the assessment of resistance strategies against Tomato yellow leaf curl virus |
| title_full | Transgenic tobacco cells: a permissive system for the assessment of resistance strategies against Tomato yellow leaf curl virus |
| title_fullStr | Transgenic tobacco cells: a permissive system for the assessment of resistance strategies against Tomato yellow leaf curl virus |
| title_full_unstemmed | Transgenic tobacco cells: a permissive system for the assessment of resistance strategies against Tomato yellow leaf curl virus |
| title_short | Transgenic tobacco cells: a permissive system for the assessment of resistance strategies against Tomato yellow leaf curl virus |
| title_sort | transgenic tobacco cells a permissive system for the assessment of resistance strategies against tomato yellow leaf curl virus |
| topic | begomovirus tyccu tabaco silenciamiento génico post-transcripcional resistencia derivada del patógeno tomate |
| url | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522009000200003&lng=en&tlng=en |
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