Effect of angiotensin II receptor blocker on TGF-β1, MMP-1, and collagen type I and type III concentration in New Zealand rabbit urethral stricture model
Safendra Siregar, Reni Farenia, Suwandi Sugandi, Rully M Roesli Urology Division, Department of Surgery, Hasan Sadikin Hospital Bandung, Faculty of Medicine Padjadjaran University, Bandung, Indonesia Introduction: Urethral stricture is a disease with a high recurrence rate. Angiotensin II via AT1 re...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2018-10-01
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| Series: | Research and Reports in Urology |
| Subjects: | |
| Online Access: | https://www.dovepress.com/effect-of-angiotensin-ii-receptor-blocker-on-tgf-beta1-mmp-1-and-colla-peer-reviewed-article-RRU |
| _version_ | 1819153399890837504 |
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| author | Siregar S Farenia R Sugandi S Roesli RM |
| author_facet | Siregar S Farenia R Sugandi S Roesli RM |
| author_sort | Siregar S |
| collection | DOAJ |
| description | Safendra Siregar, Reni Farenia, Suwandi Sugandi, Rully M Roesli Urology Division, Department of Surgery, Hasan Sadikin Hospital Bandung, Faculty of Medicine Padjadjaran University, Bandung, Indonesia Introduction: Urethral stricture is a disease with a high recurrence rate. Angiotensin II via AT1 receptor increases collagen formation through its effects on TGF-β1 and inhibition of collagenase activity. In this study, we evaluated the antifibrotic effect of angiotensin II receptor blocker on urethral stricture formation by creating a urethral stricture model in a male rabbit.Material and methods: Thirty three male adult rabbits were separated into 3 groups (control, treatment, and sham). Group I consisted of 15 rabbits with urethral stricture that did not undergo any treatment, group II consisted of 15 rabbits with urethral stricture that were treated with a daily dose of 15 mg/kg losartan, given orally. Group III consisted of 3 rabbits with normal urethra and without any treatment. After 1, 2, and 4 weeks, the urethral tissues were collected, processed, and examined for TGF-β1, MMP-1, collagen type I, and collagen type III using enzyme-linked immunosorbent assay. Data were analyzed using 2-way analysis of variance using SPSS version 20.0.Results: Urethral TGF-β1 concentration in the treatment group was significantly lower during the 2nd and 4th week of observation (p<0.0001), MMP-1 was significantly higher in the 1st, 2nd, and 4th week of observation (p<0.0001), collagen type I was significantly lower during the 2nd (p=0.001) and 4th week (p<0.0001), and collagen type III concentration was significantly lower in the 2nd and 4th week of observation (p<0.0001).Conclusion: Angiotensin II receptor blocker could limit the progression of urethral stricture. The mechanism may be related to the AT1 blockage that leads to a decrease in TGF-β1 concentration, eventually resulting in lower collagen concentration due to increased MMP-1 activity. Keywords: angiotensin receptor blocker, collagen, MMP-1, TGF-β1, urethral stricture |
| first_indexed | 2024-12-22T15:04:35Z |
| format | Article |
| id | doaj.art-28987613b6a64d938e5436334d240d45 |
| institution | Directory Open Access Journal |
| issn | 2253-2447 |
| language | English |
| last_indexed | 2024-12-22T15:04:35Z |
| publishDate | 2018-10-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Research and Reports in Urology |
| spelling | doaj.art-28987613b6a64d938e5436334d240d452022-12-21T18:22:01ZengDove Medical PressResearch and Reports in Urology2253-24472018-10-01Volume 1012713341069Effect of angiotensin II receptor blocker on TGF-β1, MMP-1, and collagen type I and type III concentration in New Zealand rabbit urethral stricture modelSiregar SFarenia RSugandi SRoesli RMSafendra Siregar, Reni Farenia, Suwandi Sugandi, Rully M Roesli Urology Division, Department of Surgery, Hasan Sadikin Hospital Bandung, Faculty of Medicine Padjadjaran University, Bandung, Indonesia Introduction: Urethral stricture is a disease with a high recurrence rate. Angiotensin II via AT1 receptor increases collagen formation through its effects on TGF-β1 and inhibition of collagenase activity. In this study, we evaluated the antifibrotic effect of angiotensin II receptor blocker on urethral stricture formation by creating a urethral stricture model in a male rabbit.Material and methods: Thirty three male adult rabbits were separated into 3 groups (control, treatment, and sham). Group I consisted of 15 rabbits with urethral stricture that did not undergo any treatment, group II consisted of 15 rabbits with urethral stricture that were treated with a daily dose of 15 mg/kg losartan, given orally. Group III consisted of 3 rabbits with normal urethra and without any treatment. After 1, 2, and 4 weeks, the urethral tissues were collected, processed, and examined for TGF-β1, MMP-1, collagen type I, and collagen type III using enzyme-linked immunosorbent assay. Data were analyzed using 2-way analysis of variance using SPSS version 20.0.Results: Urethral TGF-β1 concentration in the treatment group was significantly lower during the 2nd and 4th week of observation (p<0.0001), MMP-1 was significantly higher in the 1st, 2nd, and 4th week of observation (p<0.0001), collagen type I was significantly lower during the 2nd (p=0.001) and 4th week (p<0.0001), and collagen type III concentration was significantly lower in the 2nd and 4th week of observation (p<0.0001).Conclusion: Angiotensin II receptor blocker could limit the progression of urethral stricture. The mechanism may be related to the AT1 blockage that leads to a decrease in TGF-β1 concentration, eventually resulting in lower collagen concentration due to increased MMP-1 activity. Keywords: angiotensin receptor blocker, collagen, MMP-1, TGF-β1, urethral stricturehttps://www.dovepress.com/effect-of-angiotensin-ii-receptor-blocker-on-tgf-beta1-mmp-1-and-colla-peer-reviewed-article-RRUAngiotensin Receptor BlockerCollagenMMP-1TGF-β1Urethral stricture |
| spellingShingle | Siregar S Farenia R Sugandi S Roesli RM Effect of angiotensin II receptor blocker on TGF-β1, MMP-1, and collagen type I and type III concentration in New Zealand rabbit urethral stricture model Research and Reports in Urology Angiotensin Receptor Blocker Collagen MMP-1 TGF-β1 Urethral stricture |
| title | Effect of angiotensin II receptor blocker on TGF-β1, MMP-1, and collagen type I and type III concentration in New Zealand rabbit urethral stricture model |
| title_full | Effect of angiotensin II receptor blocker on TGF-β1, MMP-1, and collagen type I and type III concentration in New Zealand rabbit urethral stricture model |
| title_fullStr | Effect of angiotensin II receptor blocker on TGF-β1, MMP-1, and collagen type I and type III concentration in New Zealand rabbit urethral stricture model |
| title_full_unstemmed | Effect of angiotensin II receptor blocker on TGF-β1, MMP-1, and collagen type I and type III concentration in New Zealand rabbit urethral stricture model |
| title_short | Effect of angiotensin II receptor blocker on TGF-β1, MMP-1, and collagen type I and type III concentration in New Zealand rabbit urethral stricture model |
| title_sort | effect of angiotensin ii receptor blocker on tgf beta 1 mmp 1 and collagen type i and type iii concentration in new zealand rabbit urethral stricture model |
| topic | Angiotensin Receptor Blocker Collagen MMP-1 TGF-β1 Urethral stricture |
| url | https://www.dovepress.com/effect-of-angiotensin-ii-receptor-blocker-on-tgf-beta1-mmp-1-and-colla-peer-reviewed-article-RRU |
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