Characterization of the Fecal Microbiome in Dogs Receiving Medical Management for Congenital Portosystemic Shunts
BackgroundThe GI microbiome has not been characterized in dogs being medically managed for congenital portosystemic shunts (CPSS).ObjectivesTo characterize the fecal microbiome in a population of dogs being medically managed for CPSS.Animals27 client-owned dogs.MethodsProspective cohort study enroll...
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.897760/full |
| _version_ | 1828777302662578176 |
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| author | Nathan Squire Cassie Lux Katie Tolbert Jonathan Lidbury Xiaocun Sun Jan S. Suchodolski |
| author_facet | Nathan Squire Cassie Lux Katie Tolbert Jonathan Lidbury Xiaocun Sun Jan S. Suchodolski |
| author_sort | Nathan Squire |
| collection | DOAJ |
| description | BackgroundThe GI microbiome has not been characterized in dogs being medically managed for congenital portosystemic shunts (CPSS).ObjectivesTo characterize the fecal microbiome in a population of dogs being medically managed for CPSS.Animals27 client-owned dogs.MethodsProspective cohort study enrollment of fecal samples was performed with follow-up data collected retrospectively. The overall fecal dysbiosis index (DI) and individual bacterial abundances were determined using real-time qPCR. Medical management, clinical findings, clinicopathologic, and outcome variables were collected, and logistic regression analyses were performed to evaluate associations between these variables and overall DI and bacterial abundances. Numerical variables were evaluated with general linear models for normality and equal variance using Shapiro-Wilk test and Levene's test, respectively.ResultsAll dogs were administered a hepatic diet and lactulose, while antibiotics were used in 22 (81.5%) and acid suppressants in 7 (25.9%). Seventeen dogs (63.0%) had a DI >2. The median DI in this population was 3.02 (range 4.23–8.42), and the median DI in dogs receiving and not receiving antibiotics was 4.3 (range −4.23–8.42) and 1.52 (range −1.62–5.43), respectively. No significant association between any of the analyzed variables and the DI was identified. There was a significant association between the use of metronidazole and a larger abundance of E. coli (p = 0.024).Conclusions and Clinical ImportanceDysbiosis appears to be common in dogs that are being medically managed for CPSS, though the clinical significance remains unclear. |
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| id | doaj.art-289922e6265b4b44a8de92c3764b40a5 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-11T16:17:57Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-289922e6265b4b44a8de92c3764b40a52022-12-22T00:58:54ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-07-01910.3389/fvets.2022.897760897760Characterization of the Fecal Microbiome in Dogs Receiving Medical Management for Congenital Portosystemic ShuntsNathan Squire0Cassie Lux1Katie Tolbert2Jonathan Lidbury3Xiaocun Sun4Jan S. Suchodolski5Department of Small Animal Clinical Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, TN, United StatesDepartment of Small Animal Clinical Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, TN, United StatesDepartment of Veterinary Small Animal Clinical Sciences, Texas A&M College of Veterinary Medicine and Biomedical Sciences, College Station, TX, United StatesDepartment of Veterinary Small Animal Clinical Sciences, Texas A&M College of Veterinary Medicine and Biomedical Sciences, College Station, TX, United StatesOffice of Information and Technology, University of Tennessee Knoxville, Knoxville, TN, United StatesDepartment of Veterinary Small Animal Clinical Sciences, Texas A&M College of Veterinary Medicine and Biomedical Sciences, College Station, TX, United StatesBackgroundThe GI microbiome has not been characterized in dogs being medically managed for congenital portosystemic shunts (CPSS).ObjectivesTo characterize the fecal microbiome in a population of dogs being medically managed for CPSS.Animals27 client-owned dogs.MethodsProspective cohort study enrollment of fecal samples was performed with follow-up data collected retrospectively. The overall fecal dysbiosis index (DI) and individual bacterial abundances were determined using real-time qPCR. Medical management, clinical findings, clinicopathologic, and outcome variables were collected, and logistic regression analyses were performed to evaluate associations between these variables and overall DI and bacterial abundances. Numerical variables were evaluated with general linear models for normality and equal variance using Shapiro-Wilk test and Levene's test, respectively.ResultsAll dogs were administered a hepatic diet and lactulose, while antibiotics were used in 22 (81.5%) and acid suppressants in 7 (25.9%). Seventeen dogs (63.0%) had a DI >2. The median DI in this population was 3.02 (range 4.23–8.42), and the median DI in dogs receiving and not receiving antibiotics was 4.3 (range −4.23–8.42) and 1.52 (range −1.62–5.43), respectively. No significant association between any of the analyzed variables and the DI was identified. There was a significant association between the use of metronidazole and a larger abundance of E. coli (p = 0.024).Conclusions and Clinical ImportanceDysbiosis appears to be common in dogs that are being medically managed for CPSS, though the clinical significance remains unclear.https://www.frontiersin.org/articles/10.3389/fvets.2022.897760/fulldysbiosis indexhepatic encephalopathy (HE)lactulosequantitative polymerase chain reaction (qPCR)portosystemic shunt (CPSS) |
| spellingShingle | Nathan Squire Cassie Lux Katie Tolbert Jonathan Lidbury Xiaocun Sun Jan S. Suchodolski Characterization of the Fecal Microbiome in Dogs Receiving Medical Management for Congenital Portosystemic Shunts Frontiers in Veterinary Science dysbiosis index hepatic encephalopathy (HE) lactulose quantitative polymerase chain reaction (qPCR) portosystemic shunt (CPSS) |
| title | Characterization of the Fecal Microbiome in Dogs Receiving Medical Management for Congenital Portosystemic Shunts |
| title_full | Characterization of the Fecal Microbiome in Dogs Receiving Medical Management for Congenital Portosystemic Shunts |
| title_fullStr | Characterization of the Fecal Microbiome in Dogs Receiving Medical Management for Congenital Portosystemic Shunts |
| title_full_unstemmed | Characterization of the Fecal Microbiome in Dogs Receiving Medical Management for Congenital Portosystemic Shunts |
| title_short | Characterization of the Fecal Microbiome in Dogs Receiving Medical Management for Congenital Portosystemic Shunts |
| title_sort | characterization of the fecal microbiome in dogs receiving medical management for congenital portosystemic shunts |
| topic | dysbiosis index hepatic encephalopathy (HE) lactulose quantitative polymerase chain reaction (qPCR) portosystemic shunt (CPSS) |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.897760/full |
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