Elucidation of the Role of 3-Hydroxy Fatty Acids in Cryptococcus-amoeba Interactions
We previously reported that 3-hydroxy fatty acids promoted the survival of cryptococcal cells when acted upon by amoebae. To expand on this, the current study sought to explain how these molecules may protect cells. Our data suggest that 3-hydroxy fatty acids may subvert the internalization of crypt...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00765/full |
| _version_ | 1811310652021538816 |
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| author | Olihile M. Sebolai Uju L. Madu Adepemi O. Ogundeji Carolina H. Pohl Jacobus Albertyn |
| author_facet | Olihile M. Sebolai Uju L. Madu Adepemi O. Ogundeji Carolina H. Pohl Jacobus Albertyn |
| author_sort | Olihile M. Sebolai |
| collection | DOAJ |
| description | We previously reported that 3-hydroxy fatty acids promoted the survival of cryptococcal cells when acted upon by amoebae. To expand on this, the current study sought to explain how these molecules may protect cells. Our data suggest that 3-hydroxy fatty acids may subvert the internalization of cryptococcal cells via suppression of the levels of a fetuin A-like amoebal protein, which may be important for enhancing phagocytosis. Additionally, we show that an acapsular strain (that is devoid of 3-hydroxy fatty acids) was protected against the effects of hydrogen peroxide when exogenous 3-hydroxy fatty acids were present, but not in the absence of 3-hydroxy fatty acids. A similar response profile was noted when a strain with a capsule was challenged with hydrogen peroxide. We also show that cryptococcal cells that naturally produce 3-hydroxy fatty acids were more resistant to the effects of amoebapore (an amoeba-specific hydrolytic enzyme), compared to cells that do not produce these molecules. Taken together, our findings suggest that 3-hydroxy fatty acids possess an anti-phagocytic activity that may be expressed when cells interact with macrophages. This may allow the yeast cells to evade immuno-processing. |
| first_indexed | 2024-04-13T10:02:41Z |
| format | Article |
| id | doaj.art-289f5e51738a47b4aca4738639bcc365 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T10:02:41Z |
| publishDate | 2017-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-289f5e51738a47b4aca4738639bcc3652022-12-22T02:51:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-04-01810.3389/fmicb.2017.00765257955Elucidation of the Role of 3-Hydroxy Fatty Acids in Cryptococcus-amoeba InteractionsOlihile M. SebolaiUju L. MaduAdepemi O. OgundejiCarolina H. PohlJacobus AlbertynWe previously reported that 3-hydroxy fatty acids promoted the survival of cryptococcal cells when acted upon by amoebae. To expand on this, the current study sought to explain how these molecules may protect cells. Our data suggest that 3-hydroxy fatty acids may subvert the internalization of cryptococcal cells via suppression of the levels of a fetuin A-like amoebal protein, which may be important for enhancing phagocytosis. Additionally, we show that an acapsular strain (that is devoid of 3-hydroxy fatty acids) was protected against the effects of hydrogen peroxide when exogenous 3-hydroxy fatty acids were present, but not in the absence of 3-hydroxy fatty acids. A similar response profile was noted when a strain with a capsule was challenged with hydrogen peroxide. We also show that cryptococcal cells that naturally produce 3-hydroxy fatty acids were more resistant to the effects of amoebapore (an amoeba-specific hydrolytic enzyme), compared to cells that do not produce these molecules. Taken together, our findings suggest that 3-hydroxy fatty acids possess an anti-phagocytic activity that may be expressed when cells interact with macrophages. This may allow the yeast cells to evade immuno-processing.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00765/full3-hydroxy fatty acids (3-hydroxy C9:0)amoebaCryptococcusphagocytosisshield |
| spellingShingle | Olihile M. Sebolai Uju L. Madu Adepemi O. Ogundeji Carolina H. Pohl Jacobus Albertyn Elucidation of the Role of 3-Hydroxy Fatty Acids in Cryptococcus-amoeba Interactions Frontiers in Microbiology 3-hydroxy fatty acids (3-hydroxy C9:0) amoeba Cryptococcus phagocytosis shield |
| title | Elucidation of the Role of 3-Hydroxy Fatty Acids in Cryptococcus-amoeba Interactions |
| title_full | Elucidation of the Role of 3-Hydroxy Fatty Acids in Cryptococcus-amoeba Interactions |
| title_fullStr | Elucidation of the Role of 3-Hydroxy Fatty Acids in Cryptococcus-amoeba Interactions |
| title_full_unstemmed | Elucidation of the Role of 3-Hydroxy Fatty Acids in Cryptococcus-amoeba Interactions |
| title_short | Elucidation of the Role of 3-Hydroxy Fatty Acids in Cryptococcus-amoeba Interactions |
| title_sort | elucidation of the role of 3 hydroxy fatty acids in cryptococcus amoeba interactions |
| topic | 3-hydroxy fatty acids (3-hydroxy C9:0) amoeba Cryptococcus phagocytosis shield |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00765/full |
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