Studies of ticks of the genus Dermacentor (Acari; Ixodidae) on the natural occurrence of tularemia pathogen in the conditions of the Central Pre-Caucasian region
The purpose of the research is the assessment of the Francisella tularensis occurrence in nature in ticks of the genus Dermacentor; understanding the physiological age in terms of tick infection with tularemia pathogen.Materials and methods. For the period from 2015 to 2019, we examined 8449 specime...
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| Format: | Article |
| Language: | Russian |
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Federal Scientific Centre VIEV
2021-12-01
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| Series: | Российский паразитологический журнал |
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| Online Access: | https://vniigis.elpub.ru/jour/article/view/824 |
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| author | E. V. Lazarenko O. A. Gnusareva L. I. Shaposhnikova V. M. Dubyansky |
| author_facet | E. V. Lazarenko O. A. Gnusareva L. I. Shaposhnikova V. M. Dubyansky |
| author_sort | E. V. Lazarenko |
| collection | DOAJ |
| description | The purpose of the research is the assessment of the Francisella tularensis occurrence in nature in ticks of the genus Dermacentor; understanding the physiological age in terms of tick infection with tularemia pathogen.Materials and methods. For the period from 2015 to 2019, we examined 8449 specimens of Dermacentor marginatus (916 pools), 8674 specimens of D. reticulatus (705 pools), and 109 specimens of D. niveus (40 pools) for tularemia infection. To assess the dependence of tularemia pathogen found in ticks of different physiological ages, we examined 2440 specimens of D. marginatus (360 pools), and 3349 specimens of D. reticulatus (412 pools) for the period from 2016 to 2019. Studies of ixodid ticks infected with tularemia pathogen were performed by the Natural Focal Infection Laboratory of the Stavropol Anti-Plague Institute. Pools of ixodid ticks were examined for the pathogen DNA of tularemia using reagent kits for identifying Francisella tularensis DNA by polymerase chain reaction with fluorescence hybridization of results recorded in real time.Results and discussion. The infection rate of the tularemia pathogen in ticks in the Central Pre-Caucasian region ranged from 0.044–1.127% in D. marginatus and 0.035–1.455% in D. reticulatus in different years. The greatest number of F. tularensis was isolated from the III physiological age ticks. For D. reticulatus ticks, no statistically significant dependence of the detected tularemia pathogen on physiological age was found. |
| first_indexed | 2024-03-08T21:39:49Z |
| format | Article |
| id | doaj.art-28a5b3207df24eee9f7dc4cb65096f0d |
| institution | Directory Open Access Journal |
| issn | 1998-8435 2541-7843 |
| language | Russian |
| last_indexed | 2024-04-25T02:04:50Z |
| publishDate | 2021-12-01 |
| publisher | Federal Scientific Centre VIEV |
| record_format | Article |
| series | Российский паразитологический журнал |
| spelling | doaj.art-28a5b3207df24eee9f7dc4cb65096f0d2024-03-07T14:34:31ZrusFederal Scientific Centre VIEVРоссийский паразитологический журнал1998-84352541-78432021-12-01154293510.31016/1998-8435-2021-15-4-29-35662Studies of ticks of the genus Dermacentor (Acari; Ixodidae) on the natural occurrence of tularemia pathogen in the conditions of the Central Pre-Caucasian regionE. V. Lazarenko0O. A. Gnusareva1L. I. Shaposhnikova2V. M. Dubyansky3Stavropol Anti-Plague Research Institute of the Federal Service for Surveillance on Consumer Rights Protection and Human WellbeingStavropol Anti-Plague Research Institute of the Federal Service for Surveillance on Consumer Rights Protection and Human WellbeingStavropol Anti-Plague Research Institute of the Federal Service for Surveillance on Consumer Rights Protection and Human WellbeingStavropol Anti-Plague Research Institute of the Federal Service for Surveillance on Consumer Rights Protection and Human WellbeingThe purpose of the research is the assessment of the Francisella tularensis occurrence in nature in ticks of the genus Dermacentor; understanding the physiological age in terms of tick infection with tularemia pathogen.Materials and methods. For the period from 2015 to 2019, we examined 8449 specimens of Dermacentor marginatus (916 pools), 8674 specimens of D. reticulatus (705 pools), and 109 specimens of D. niveus (40 pools) for tularemia infection. To assess the dependence of tularemia pathogen found in ticks of different physiological ages, we examined 2440 specimens of D. marginatus (360 pools), and 3349 specimens of D. reticulatus (412 pools) for the period from 2016 to 2019. Studies of ixodid ticks infected with tularemia pathogen were performed by the Natural Focal Infection Laboratory of the Stavropol Anti-Plague Institute. Pools of ixodid ticks were examined for the pathogen DNA of tularemia using reagent kits for identifying Francisella tularensis DNA by polymerase chain reaction with fluorescence hybridization of results recorded in real time.Results and discussion. The infection rate of the tularemia pathogen in ticks in the Central Pre-Caucasian region ranged from 0.044–1.127% in D. marginatus and 0.035–1.455% in D. reticulatus in different years. The greatest number of F. tularensis was isolated from the III physiological age ticks. For D. reticulatus ticks, no statistically significant dependence of the detected tularemia pathogen on physiological age was found.https://vniigis.elpub.ru/jour/article/view/824ixodid ticksdermacentor reticulatusdermacentor marginatusfrancisella tularensistularemiainfection ratephysiological age |
| spellingShingle | E. V. Lazarenko O. A. Gnusareva L. I. Shaposhnikova V. M. Dubyansky Studies of ticks of the genus Dermacentor (Acari; Ixodidae) on the natural occurrence of tularemia pathogen in the conditions of the Central Pre-Caucasian region Российский паразитологический журнал ixodid ticks dermacentor reticulatus dermacentor marginatus francisella tularensis tularemia infection rate physiological age |
| title | Studies of ticks of the genus Dermacentor (Acari; Ixodidae) on the natural occurrence of tularemia pathogen in the conditions of the Central Pre-Caucasian region |
| title_full | Studies of ticks of the genus Dermacentor (Acari; Ixodidae) on the natural occurrence of tularemia pathogen in the conditions of the Central Pre-Caucasian region |
| title_fullStr | Studies of ticks of the genus Dermacentor (Acari; Ixodidae) on the natural occurrence of tularemia pathogen in the conditions of the Central Pre-Caucasian region |
| title_full_unstemmed | Studies of ticks of the genus Dermacentor (Acari; Ixodidae) on the natural occurrence of tularemia pathogen in the conditions of the Central Pre-Caucasian region |
| title_short | Studies of ticks of the genus Dermacentor (Acari; Ixodidae) on the natural occurrence of tularemia pathogen in the conditions of the Central Pre-Caucasian region |
| title_sort | studies of ticks of the genus dermacentor acari ixodidae on the natural occurrence of tularemia pathogen in the conditions of the central pre caucasian region |
| topic | ixodid ticks dermacentor reticulatus dermacentor marginatus francisella tularensis tularemia infection rate physiological age |
| url | https://vniigis.elpub.ru/jour/article/view/824 |
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