Evaluation of hydrocarbons level and identification of indigenous bacteria in soil from auto-mechanic workshops along Ikokwu Mechanic Village, Port Harcourt, Nigeria
Soil samples collected from Ikokwu auto-mechanic village Mile 3, Port Harcourt, Nigeria were analyzed for the microbiological and hydrocarbon content using standard culture dependent and physiochemical methods. Five sets of soil designated A, B, C, D and E were collected using composite technique a...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2018-02-01
|
| Series: | Journal of Applied Sciences and Environmental Management |
| Subjects: | |
| Online Access: | https://www.ajol.info/index.php/jasem/article/view/166346 |
| _version_ | 1827296984698978304 |
|---|---|
| author | O.N. Akomah A.D. Osayande |
| author_facet | O.N. Akomah A.D. Osayande |
| author_sort | O.N. Akomah |
| collection | DOAJ |
| description |
Soil samples collected from Ikokwu auto-mechanic village Mile 3, Port Harcourt, Nigeria were analyzed for the microbiological and hydrocarbon content using standard culture dependent and physiochemical methods. Five sets of soil designated A, B, C, D and E were collected using composite technique at depth 0 - 15 cm and 15 -30 cm. The parameters analyzed over an experimental period of 60 days were; total heterotrophic bacteria (THB), hydrocarbon utilizing bacteria (HUB), total petroleum hydrocarbon (TPH), polyaromatic hydrocarbon (PAH) and total hydrocarbon content (THC). The total heterotrophic bacterial count (THB) ranges from 1.36 x 106 - 1.10 x 108 cfu/g for A- E (0-15) cm and 5.0 x 105 - 7.0 x 107cfu/g for (15-30) cm while the total hydrocarbon utilizing bacteria (HUB) count ranges from 3.5 x 105 - 5.4 x 106cfu/g for (0-15)cm and 2.0 x 105 - 9.4 x 106 for (15-30) cm. The bacteria isolates included the genera Bacillus sp., Flavobacterius sp., Pseudomonas sp., Micrococcus sp., Arthrobacter sp., Acetobacter sp., Proteus sp. and Staphylcoccus sp. The TPH ranges from 34.32 - 1746.6mg/kg for (0-15) cm and 29.49 -1141.0 mg/kg for (15-30) cm. The THC ranges from 1.3 - 5.66 mg/kg for (0-15) cm and 0.84 - 2.719 mg/kg for (15-30) cm. PAH recorded a value of 3.246 - 6.890 mg/kg for (0-15) cm and 0.096 - 2.860 mg/kg for (15-30) cm. It is recommended that (i) the hydrocarbon degraders be enhance and employed for bioremediation of auto-mechanic workshop (ii) it is essential that the metagenomics of spent engine oil contaminated soil at auto-mechanic workshop be studied for optimum utilization of their potentials.
Keywords: Soils, Polyaromatic Hydrocarbons, Spent engine oil, hydrocarbon utilizing bacteria
|
| first_indexed | 2024-04-24T14:48:57Z |
| format | Article |
| id | doaj.art-28a6f46ef29642ab89ba7777d6cc4fee |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T14:48:57Z |
| publishDate | 2018-02-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-28a6f46ef29642ab89ba7777d6cc4fee2024-04-02T19:52:12ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992018-02-0122110.4314/jasem.v22i1.21Evaluation of hydrocarbons level and identification of indigenous bacteria in soil from auto-mechanic workshops along Ikokwu Mechanic Village, Port Harcourt, NigeriaO.N. AkomahA.D. Osayande Soil samples collected from Ikokwu auto-mechanic village Mile 3, Port Harcourt, Nigeria were analyzed for the microbiological and hydrocarbon content using standard culture dependent and physiochemical methods. Five sets of soil designated A, B, C, D and E were collected using composite technique at depth 0 - 15 cm and 15 -30 cm. The parameters analyzed over an experimental period of 60 days were; total heterotrophic bacteria (THB), hydrocarbon utilizing bacteria (HUB), total petroleum hydrocarbon (TPH), polyaromatic hydrocarbon (PAH) and total hydrocarbon content (THC). The total heterotrophic bacterial count (THB) ranges from 1.36 x 106 - 1.10 x 108 cfu/g for A- E (0-15) cm and 5.0 x 105 - 7.0 x 107cfu/g for (15-30) cm while the total hydrocarbon utilizing bacteria (HUB) count ranges from 3.5 x 105 - 5.4 x 106cfu/g for (0-15)cm and 2.0 x 105 - 9.4 x 106 for (15-30) cm. The bacteria isolates included the genera Bacillus sp., Flavobacterius sp., Pseudomonas sp., Micrococcus sp., Arthrobacter sp., Acetobacter sp., Proteus sp. and Staphylcoccus sp. The TPH ranges from 34.32 - 1746.6mg/kg for (0-15) cm and 29.49 -1141.0 mg/kg for (15-30) cm. The THC ranges from 1.3 - 5.66 mg/kg for (0-15) cm and 0.84 - 2.719 mg/kg for (15-30) cm. PAH recorded a value of 3.246 - 6.890 mg/kg for (0-15) cm and 0.096 - 2.860 mg/kg for (15-30) cm. It is recommended that (i) the hydrocarbon degraders be enhance and employed for bioremediation of auto-mechanic workshop (ii) it is essential that the metagenomics of spent engine oil contaminated soil at auto-mechanic workshop be studied for optimum utilization of their potentials. Keywords: Soils, Polyaromatic Hydrocarbons, Spent engine oil, hydrocarbon utilizing bacteria https://www.ajol.info/index.php/jasem/article/view/166346SoilsPolyaromatic HydrocarbonsSpent engine oilhydrocarbon utilizing bacteria |
| spellingShingle | O.N. Akomah A.D. Osayande Evaluation of hydrocarbons level and identification of indigenous bacteria in soil from auto-mechanic workshops along Ikokwu Mechanic Village, Port Harcourt, Nigeria Journal of Applied Sciences and Environmental Management Soils Polyaromatic Hydrocarbons Spent engine oil hydrocarbon utilizing bacteria |
| title | Evaluation of hydrocarbons level and identification of indigenous bacteria in soil from auto-mechanic workshops along Ikokwu Mechanic Village, Port Harcourt, Nigeria |
| title_full | Evaluation of hydrocarbons level and identification of indigenous bacteria in soil from auto-mechanic workshops along Ikokwu Mechanic Village, Port Harcourt, Nigeria |
| title_fullStr | Evaluation of hydrocarbons level and identification of indigenous bacteria in soil from auto-mechanic workshops along Ikokwu Mechanic Village, Port Harcourt, Nigeria |
| title_full_unstemmed | Evaluation of hydrocarbons level and identification of indigenous bacteria in soil from auto-mechanic workshops along Ikokwu Mechanic Village, Port Harcourt, Nigeria |
| title_short | Evaluation of hydrocarbons level and identification of indigenous bacteria in soil from auto-mechanic workshops along Ikokwu Mechanic Village, Port Harcourt, Nigeria |
| title_sort | evaluation of hydrocarbons level and identification of indigenous bacteria in soil from auto mechanic workshops along ikokwu mechanic village port harcourt nigeria |
| topic | Soils Polyaromatic Hydrocarbons Spent engine oil hydrocarbon utilizing bacteria |
| url | https://www.ajol.info/index.php/jasem/article/view/166346 |
| work_keys_str_mv | AT onakomah evaluationofhydrocarbonslevelandidentificationofindigenousbacteriainsoilfromautomechanicworkshopsalongikokwumechanicvillageportharcourtnigeria AT adosayande evaluationofhydrocarbonslevelandidentificationofindigenousbacteriainsoilfromautomechanicworkshopsalongikokwumechanicvillageportharcourtnigeria |