Natural deep eutectic solvent extraction of xanthorrhizol and curcuminoids from Curcuma xanthorrhiza Roxb and simultaneous determination by high-performance liquid chromatography
Context: Curcuma xanthorrhiza, a native to Indonesia, has been recognized as a potential medicinal plant. Xanthorrhizol (XTZ), curcumin (Cur), bisdemethoxycurcumin (BDMC), and demethoxycurcumin (DMC) are the main major metabolites with different bioactivities. Thus, the selective extraction of these...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
GarVal Editorial Ltda.
2023-11-01
|
| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | https://jppres.com/jppres/pdf/vol11/jppres23.1727_11.6.1056.pdf |
| _version_ | 1797363861257453568 |
|---|---|
| author | Adelina Simamora Kris H. Timotius Heri Setiawan Masteria Y. Putra Abdul Mun’im |
| author_facet | Adelina Simamora Kris H. Timotius Heri Setiawan Masteria Y. Putra Abdul Mun’im |
| author_sort | Adelina Simamora |
| collection | DOAJ |
| description | Context: Curcuma xanthorrhiza, a native to Indonesia, has been recognized as a potential medicinal plant. Xanthorrhizol (XTZ), curcumin (Cur), bisdemethoxycurcumin (BDMC), and demethoxycurcumin (DMC) are the main major metabolites with different bioactivities. Thus, the selective extraction of these markers is required.
Aims: To characterize and use natural deep eutectic solvents (NADES) for the extraction of XTZ and curcuminoids (CURS) from C. xanthorrhiza.
Methods: Four NADES, comprising of glucose:lactic acid (Glu:LA, 1:3), choline chloride: malic acid (ChCl:MA, 1:1), choline chloride: glycerol (ChCl:Gly, 1:1), and choline chloride: sucrose (ChCl:Suc, 3:1) were synthesized and subjected to FTIR. The physicochemical properties of NADES were characterized, including polarity, viscosity, and density. Their antioxidant profiles were evaluated. The toxicity of NADES was assessed towards Escherichia coli by real-time growth monitoring. Tested NADES were employed to extract marker compounds from the rhizome of C. xanthorrhiza. A validated high-performance liquid chromatography (HPLC) method was applied to simultaneously quantify XTZ and CURS in NADES extracts.
Results: FTIR spectra confirmed the formation of hydrogen bonds between components. Using the DPPH and FRAP methods, all NADES and NADES extracts displayed antioxidative activity, suggesting the contribution of NADES to the activity. Of the tested NADES, Glu:LA (1:3) obtained the highest content of XTZ over Cur, BDMC, and DMC, comparable to that of ethanol maceration. All tested NADES were harmless to E. coli, except for ChCl:MA (1:1). However, NADES extracts of Glu:LA (1:3) and ChCl:MA (1:1) suppressed the growth of the bacterium. Scanning electron micrographs demonstrated the destruction of the surface structure of the rhizome powder by NADES-ultrasonication.
Conclusions: The study recommended the use of Glu:LA (1:3) to selectively extract XTZ to promote environmentally friendly and sustainable extraction. |
| first_indexed | 2024-03-08T16:27:02Z |
| format | Article |
| id | doaj.art-28a75398599f4b5dabdfbc2edf2705c1 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-03-08T16:27:02Z |
| publishDate | 2023-11-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-28a75398599f4b5dabdfbc2edf2705c12024-01-06T17:07:01ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502023-11-011161056107110.56499/jppres23.1727_11.6.1056Natural deep eutectic solvent extraction of xanthorrhizol and curcuminoids from Curcuma xanthorrhiza Roxb and simultaneous determination by high-performance liquid chromatographyAdelina Simamora0Kris H. Timotius1Heri Setiawan2Masteria Y. Putra3Abdul Mun’im4Graduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Indonesia, 16424, Depok, Indonesia. Department of Biochemistry, Faculty of Medicine and Health Sciences, Krida Wacana Christian University, 11510, Jakarta, Indonesia. National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, 16424, Depok, West Java, Indonesia.Department of Biochemistry, Faculty of Medicine and Health Sciences, Krida Wacana Christian University, 11510, Jakarta, Indonesia.National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, 16424, Depok, West Java, Indonesia. Department of Pharmacology, Faculty of Pharmacy, Universitas Indonesia, 16424, Depok, Indonesia.National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, 16424, Depok, West Java, Indonesia. Research Center for Vaccine and Drug, Research Organization for Health, National Research and Innovation Agency (BRIN), 16911, Cibinong, West Java, Indonesia.National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, 16424, Depok, West Java, Indonesia. Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, 16424, Depok, Indonesia.Context: Curcuma xanthorrhiza, a native to Indonesia, has been recognized as a potential medicinal plant. Xanthorrhizol (XTZ), curcumin (Cur), bisdemethoxycurcumin (BDMC), and demethoxycurcumin (DMC) are the main major metabolites with different bioactivities. Thus, the selective extraction of these markers is required. Aims: To characterize and use natural deep eutectic solvents (NADES) for the extraction of XTZ and curcuminoids (CURS) from C. xanthorrhiza. Methods: Four NADES, comprising of glucose:lactic acid (Glu:LA, 1:3), choline chloride: malic acid (ChCl:MA, 1:1), choline chloride: glycerol (ChCl:Gly, 1:1), and choline chloride: sucrose (ChCl:Suc, 3:1) were synthesized and subjected to FTIR. The physicochemical properties of NADES were characterized, including polarity, viscosity, and density. Their antioxidant profiles were evaluated. The toxicity of NADES was assessed towards Escherichia coli by real-time growth monitoring. Tested NADES were employed to extract marker compounds from the rhizome of C. xanthorrhiza. A validated high-performance liquid chromatography (HPLC) method was applied to simultaneously quantify XTZ and CURS in NADES extracts. Results: FTIR spectra confirmed the formation of hydrogen bonds between components. Using the DPPH and FRAP methods, all NADES and NADES extracts displayed antioxidative activity, suggesting the contribution of NADES to the activity. Of the tested NADES, Glu:LA (1:3) obtained the highest content of XTZ over Cur, BDMC, and DMC, comparable to that of ethanol maceration. All tested NADES were harmless to E. coli, except for ChCl:MA (1:1). However, NADES extracts of Glu:LA (1:3) and ChCl:MA (1:1) suppressed the growth of the bacterium. Scanning electron micrographs demonstrated the destruction of the surface structure of the rhizome powder by NADES-ultrasonication. Conclusions: The study recommended the use of Glu:LA (1:3) to selectively extract XTZ to promote environmentally friendly and sustainable extraction.https://jppres.com/jppres/pdf/vol11/jppres23.1727_11.6.1056.pdfescherichia coliscanning electron microscopyterpenoidtoxicity testviscosity |
| spellingShingle | Adelina Simamora Kris H. Timotius Heri Setiawan Masteria Y. Putra Abdul Mun’im Natural deep eutectic solvent extraction of xanthorrhizol and curcuminoids from Curcuma xanthorrhiza Roxb and simultaneous determination by high-performance liquid chromatography Journal of Pharmacy & Pharmacognosy Research escherichia coli scanning electron microscopy terpenoid toxicity test viscosity |
| title | Natural deep eutectic solvent extraction of xanthorrhizol and curcuminoids from Curcuma xanthorrhiza Roxb and simultaneous determination by high-performance liquid chromatography |
| title_full | Natural deep eutectic solvent extraction of xanthorrhizol and curcuminoids from Curcuma xanthorrhiza Roxb and simultaneous determination by high-performance liquid chromatography |
| title_fullStr | Natural deep eutectic solvent extraction of xanthorrhizol and curcuminoids from Curcuma xanthorrhiza Roxb and simultaneous determination by high-performance liquid chromatography |
| title_full_unstemmed | Natural deep eutectic solvent extraction of xanthorrhizol and curcuminoids from Curcuma xanthorrhiza Roxb and simultaneous determination by high-performance liquid chromatography |
| title_short | Natural deep eutectic solvent extraction of xanthorrhizol and curcuminoids from Curcuma xanthorrhiza Roxb and simultaneous determination by high-performance liquid chromatography |
| title_sort | natural deep eutectic solvent extraction of xanthorrhizol and curcuminoids from curcuma xanthorrhiza roxb and simultaneous determination by high performance liquid chromatography |
| topic | escherichia coli scanning electron microscopy terpenoid toxicity test viscosity |
| url | https://jppres.com/jppres/pdf/vol11/jppres23.1727_11.6.1056.pdf |
| work_keys_str_mv | AT adelinasimamora naturaldeepeutecticsolventextractionofxanthorrhizolandcurcuminoidsfromcurcumaxanthorrhizaroxbandsimultaneousdeterminationbyhighperformanceliquidchromatography AT krishtimotius naturaldeepeutecticsolventextractionofxanthorrhizolandcurcuminoidsfromcurcumaxanthorrhizaroxbandsimultaneousdeterminationbyhighperformanceliquidchromatography AT herisetiawan naturaldeepeutecticsolventextractionofxanthorrhizolandcurcuminoidsfromcurcumaxanthorrhizaroxbandsimultaneousdeterminationbyhighperformanceliquidchromatography AT masteriayputra naturaldeepeutecticsolventextractionofxanthorrhizolandcurcuminoidsfromcurcumaxanthorrhizaroxbandsimultaneousdeterminationbyhighperformanceliquidchromatography AT abdulmunim naturaldeepeutecticsolventextractionofxanthorrhizolandcurcuminoidsfromcurcumaxanthorrhizaroxbandsimultaneousdeterminationbyhighperformanceliquidchromatography |