Nanosheet-Facilitated Spray Delivery of dsRNAs Represents a Potential Tool to Control <i>Rhizoctonia solani</i> Infection
<i>Rhizoctonia solani</i> is one of the important pathogenic fungi causing several serious crop diseases, such as maize and rice sheath blight. Current methods used to control the disease mainly depend on spraying fungicides because there is no immunity or high resistance available in cr...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/12922 |
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| author | Xijun Chen Tong Shi Tao Tang Chen Chen You Liang Shimin Zuo |
| author_facet | Xijun Chen Tong Shi Tao Tang Chen Chen You Liang Shimin Zuo |
| author_sort | Xijun Chen |
| collection | DOAJ |
| description | <i>Rhizoctonia solani</i> is one of the important pathogenic fungi causing several serious crop diseases, such as maize and rice sheath blight. Current methods used to control the disease mainly depend on spraying fungicides because there is no immunity or high resistance available in crops. Spraying double-strand RNA (dsRNA) for induced-gene silencing (SIGS) is a new potentially sustainable and environmentally friendly tool to control plant diseases. Here, we found that fluorescein-labelled <i>EGFP</i>-dsRNA could be absorbed by <i>R. solani</i> in co-incubation. Furthermore, three dsRNAs, each targeting one of pathogenicity-related genes, <i>RsPG1</i>, <i>RsCATA,</i> and <i>RsCRZ1</i>, significantly downregulated the transcript levels of the target genes after co-incubation, leading to a significant reduction in the pathogenicity of the fungus. Only the spray of <i>RsCRZ1</i> dsRNA, but not <i>RsPG1</i> or <i>RsCATA</i> dsRNA, affected fungal sclerotium formation. dsRNA stability on leaf surfaces and its efficiency in entering leaf cells were significantly improved when dsRNAs were loaded on layered double hydroxide (LDH) nanosheets. Notably, the <i>RsCRZ1-dsRNA-LDH</i> approach showed stronger and more lasting effects than using <i>RsCRZ1-dsRNA</i> alone in controlling pathogen development. Together, this study provides a new potential method to control crop diseases caused by <i>R. solani</i>. |
| first_indexed | 2024-03-09T19:01:20Z |
| format | Article |
| id | doaj.art-28a9489802ab41c9b60341747c7c5fb9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:01:20Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-28a9489802ab41c9b60341747c7c5fb92023-11-24T04:59:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123211292210.3390/ijms232112922Nanosheet-Facilitated Spray Delivery of dsRNAs Represents a Potential Tool to Control <i>Rhizoctonia solani</i> InfectionXijun Chen0Tong Shi1Tao Tang2Chen Chen3You Liang4Shimin Zuo5College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou 225009, China<i>Rhizoctonia solani</i> is one of the important pathogenic fungi causing several serious crop diseases, such as maize and rice sheath blight. Current methods used to control the disease mainly depend on spraying fungicides because there is no immunity or high resistance available in crops. Spraying double-strand RNA (dsRNA) for induced-gene silencing (SIGS) is a new potentially sustainable and environmentally friendly tool to control plant diseases. Here, we found that fluorescein-labelled <i>EGFP</i>-dsRNA could be absorbed by <i>R. solani</i> in co-incubation. Furthermore, three dsRNAs, each targeting one of pathogenicity-related genes, <i>RsPG1</i>, <i>RsCATA,</i> and <i>RsCRZ1</i>, significantly downregulated the transcript levels of the target genes after co-incubation, leading to a significant reduction in the pathogenicity of the fungus. Only the spray of <i>RsCRZ1</i> dsRNA, but not <i>RsPG1</i> or <i>RsCATA</i> dsRNA, affected fungal sclerotium formation. dsRNA stability on leaf surfaces and its efficiency in entering leaf cells were significantly improved when dsRNAs were loaded on layered double hydroxide (LDH) nanosheets. Notably, the <i>RsCRZ1-dsRNA-LDH</i> approach showed stronger and more lasting effects than using <i>RsCRZ1-dsRNA</i> alone in controlling pathogen development. Together, this study provides a new potential method to control crop diseases caused by <i>R. solani</i>.https://www.mdpi.com/1422-0067/23/21/12922spray-induced gene silencing (SIGS)double-stranded RNA (dsRNA)disease controlling<i>Rhizoctonia solani</i> |
| spellingShingle | Xijun Chen Tong Shi Tao Tang Chen Chen You Liang Shimin Zuo Nanosheet-Facilitated Spray Delivery of dsRNAs Represents a Potential Tool to Control <i>Rhizoctonia solani</i> Infection International Journal of Molecular Sciences spray-induced gene silencing (SIGS) double-stranded RNA (dsRNA) disease controlling <i>Rhizoctonia solani</i> |
| title | Nanosheet-Facilitated Spray Delivery of dsRNAs Represents a Potential Tool to Control <i>Rhizoctonia solani</i> Infection |
| title_full | Nanosheet-Facilitated Spray Delivery of dsRNAs Represents a Potential Tool to Control <i>Rhizoctonia solani</i> Infection |
| title_fullStr | Nanosheet-Facilitated Spray Delivery of dsRNAs Represents a Potential Tool to Control <i>Rhizoctonia solani</i> Infection |
| title_full_unstemmed | Nanosheet-Facilitated Spray Delivery of dsRNAs Represents a Potential Tool to Control <i>Rhizoctonia solani</i> Infection |
| title_short | Nanosheet-Facilitated Spray Delivery of dsRNAs Represents a Potential Tool to Control <i>Rhizoctonia solani</i> Infection |
| title_sort | nanosheet facilitated spray delivery of dsrnas represents a potential tool to control i rhizoctonia solani i infection |
| topic | spray-induced gene silencing (SIGS) double-stranded RNA (dsRNA) disease controlling <i>Rhizoctonia solani</i> |
| url | https://www.mdpi.com/1422-0067/23/21/12922 |
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