Coptis Chinensis affects the function of glioma cells through the down-regulation of phosphorylation of STAT3 by reducing HDAC3
Abstract Background Glioma remains the most common cause of brain cancer-related mortality. Glioma accounts for 50–60% of brain cancer. Due to their low toxicity and infrequent side effects, traditional herbs have been increasingly popular. Coptis Chinensis is commonly used in cancer treatment in co...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-017-2029-0 |
| _version_ | 1818512306970034176 |
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| author | Jiangan Li Lulu Ni Bing Li Mingdeng Wang Zhemin Ding Chunrong Xiong Xiaojie Lu |
| author_facet | Jiangan Li Lulu Ni Bing Li Mingdeng Wang Zhemin Ding Chunrong Xiong Xiaojie Lu |
| author_sort | Jiangan Li |
| collection | DOAJ |
| description | Abstract Background Glioma remains the most common cause of brain cancer-related mortality. Glioma accounts for 50–60% of brain cancer. Due to their low toxicity and infrequent side effects, traditional herbs have been increasingly popular. Coptis Chinensis is commonly used in cancer treatment in combination with other Chinese Medicine herbs. However, little is known about its biological functions and mechanisms in glioma cells. Methods In this study, the anti-glioma cell effect of Coptis Chinensis was determined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) method, plate clone test, scratch tests, flow cytometry, western blotting and a glioma xenograft tumor model. Results The results showed that Coptis Chinensis significantly suppressed glioma cell proliferation, tumor formation, migration and tumor growth, and prolonged the survival time of glioma cell-bearing mice. The flow cytometry result showed that Coptis Chinensis induced cell cycle arrest and apoptosis in glioma cells. Western blotting showed that Coptis Chinensis down-regulated the Signal transducer and activator of transcription 3 (STAT3) phosphorylation levels and reduced the expression of Histone deacetylase 3 (HDAC3) and caspase 3. Conclusions Coptis Chinensis can inhibit various aspects of glioma cell functions. This study provides favorable scientific evidence for the potential use of natural products such as Coptis Chinensis in the clinical treatment of patients with glioma. |
| first_indexed | 2024-12-10T23:45:02Z |
| format | Article |
| id | doaj.art-28aa16c6455448ac839400c394c9b319 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-10T23:45:02Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-28aa16c6455448ac839400c394c9b3192022-12-22T01:28:57ZengBMCBMC Complementary and Alternative Medicine1472-68822017-12-0117111210.1186/s12906-017-2029-0Coptis Chinensis affects the function of glioma cells through the down-regulation of phosphorylation of STAT3 by reducing HDAC3Jiangan Li0Lulu Ni1Bing Li2Mingdeng Wang3Zhemin Ding4Chunrong Xiong5Xiaojie Lu6Department of Neurosurgery, Wuxi No.2 Hospital Affiliated to Nanjing Medical UniversityDepartment of Traditional Chinese medicin, the Affiliated Hospital of Jiangnan University ( Wuxi No.4 People’s Hospital)Department of Neurosurgery, Wuxi No.2 Hospital Affiliated to Nanjing Medical UniversityDepartment of Emergency medicine, Suzhou science and technology town hospitalDepartment of Traditional Chinese medicin, the Affiliated Hospital of Jiangnan University ( Wuxi No.4 People’s Hospital)Research Institute of schistosomiasis control in Jiangsu ProvinceDepartment of Neurosurgery, Wuxi No.2 Hospital Affiliated to Nanjing Medical UniversityAbstract Background Glioma remains the most common cause of brain cancer-related mortality. Glioma accounts for 50–60% of brain cancer. Due to their low toxicity and infrequent side effects, traditional herbs have been increasingly popular. Coptis Chinensis is commonly used in cancer treatment in combination with other Chinese Medicine herbs. However, little is known about its biological functions and mechanisms in glioma cells. Methods In this study, the anti-glioma cell effect of Coptis Chinensis was determined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) method, plate clone test, scratch tests, flow cytometry, western blotting and a glioma xenograft tumor model. Results The results showed that Coptis Chinensis significantly suppressed glioma cell proliferation, tumor formation, migration and tumor growth, and prolonged the survival time of glioma cell-bearing mice. The flow cytometry result showed that Coptis Chinensis induced cell cycle arrest and apoptosis in glioma cells. Western blotting showed that Coptis Chinensis down-regulated the Signal transducer and activator of transcription 3 (STAT3) phosphorylation levels and reduced the expression of Histone deacetylase 3 (HDAC3) and caspase 3. Conclusions Coptis Chinensis can inhibit various aspects of glioma cell functions. This study provides favorable scientific evidence for the potential use of natural products such as Coptis Chinensis in the clinical treatment of patients with glioma.http://link.springer.com/article/10.1186/s12906-017-2029-0Coptis ChinensisGlioma cellsApoptosisHDAC3P-STAT3 |
| spellingShingle | Jiangan Li Lulu Ni Bing Li Mingdeng Wang Zhemin Ding Chunrong Xiong Xiaojie Lu Coptis Chinensis affects the function of glioma cells through the down-regulation of phosphorylation of STAT3 by reducing HDAC3 BMC Complementary and Alternative Medicine Coptis Chinensis Glioma cells Apoptosis HDAC3 P-STAT3 |
| title | Coptis Chinensis affects the function of glioma cells through the down-regulation of phosphorylation of STAT3 by reducing HDAC3 |
| title_full | Coptis Chinensis affects the function of glioma cells through the down-regulation of phosphorylation of STAT3 by reducing HDAC3 |
| title_fullStr | Coptis Chinensis affects the function of glioma cells through the down-regulation of phosphorylation of STAT3 by reducing HDAC3 |
| title_full_unstemmed | Coptis Chinensis affects the function of glioma cells through the down-regulation of phosphorylation of STAT3 by reducing HDAC3 |
| title_short | Coptis Chinensis affects the function of glioma cells through the down-regulation of phosphorylation of STAT3 by reducing HDAC3 |
| title_sort | coptis chinensis affects the function of glioma cells through the down regulation of phosphorylation of stat3 by reducing hdac3 |
| topic | Coptis Chinensis Glioma cells Apoptosis HDAC3 P-STAT3 |
| url | http://link.springer.com/article/10.1186/s12906-017-2029-0 |
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