Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (<i>Polygonum multiflorum</i>) Developed Using an In Vitro Culture Method
<i>Polygonum multiflorum</i> Thunb. is a perennial plant that belongs to Polygonaceae. Root tissues are the main plant parts used as medicinal herbs in Korean oriental medicine. The <i>P. multiflorum</i> tuber is well known for its medicinal properties in Korean oriental medi...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/10/1280 |
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| author | Yong-Goo Kim Richard Komakech Dae Hui Jeong Kwonseok Jeon Yunmi Park Tae Kyoung Lee Ki Hyun Kim Byeong Cheol Moon Youngmin Kang |
| author_facet | Yong-Goo Kim Richard Komakech Dae Hui Jeong Kwonseok Jeon Yunmi Park Tae Kyoung Lee Ki Hyun Kim Byeong Cheol Moon Youngmin Kang |
| author_sort | Yong-Goo Kim |
| collection | DOAJ |
| description | <i>Polygonum multiflorum</i> Thunb. is a perennial plant that belongs to Polygonaceae. Root tissues are the main plant parts used as medicinal herbs in Korean oriental medicine. The <i>P. multiflorum</i> tuber is well known for its medicinal properties in Korean oriental medicine, and it contains a number of useful substances (secondary metabolites of emodin, 2,3,5,4′-tetrahydroxystilbene-2-<i>O</i>-<i>β</i>-<span style="font-variant: small-caps;">d</span>-glucoside (TSG), etc.) that are increasing in demand, as several studies show that they have beneficial effects on the human body. In this study, the production volumes and useful material content differences between cultured <i>P. multiflorum</i> seedlings (culture seedlings: CSs), which had been grown using a tissue culture technique under optimized conditions, and existing varieties in circulation (seed seedlings: SSs) were determined using a long-term field test. The growth characteristics of the underground parts were investigated by harvesting the tuberous roots (medicinal parts) after 1 year, and the results showed that the fresh and dry weights of the CS tubers were higher than those of the SS tubers. However, the SS rootlets had higher fresh and dry weights than the CS rootlets. A liquid chromatography-mass spectrometry component analysis of the <i>P. multiflorum</i> tubers and a Fourier transform near-infrared spectrophotometer analysis of the roots were undertaken. The results showed that the levels of TSG, which is a medicinal substance produced by <i>P. multiflorum</i>, were higher in the CSs than in the SSs, but the differences were not significant. The CS results from this study will inform future studies on the mass production of <i>P. multiflorum</i> in the field because the medicinal area was greater in CSs than in SSs. |
| first_indexed | 2024-03-10T15:59:31Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T15:59:31Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-28ab052e49b6421ab17d023fbce3a6532023-11-20T15:23:53ZengMDPI AGPlants2223-77472020-09-01910128010.3390/plants9101280Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (<i>Polygonum multiflorum</i>) Developed Using an In Vitro Culture MethodYong-Goo Kim0Richard Komakech1Dae Hui Jeong2Kwonseok Jeon3Yunmi Park4Tae Kyoung Lee5Ki Hyun Kim6Byeong Cheol Moon7Youngmin Kang8Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine (KIOM), 111 Geonjae-ro, Naju-si 58245, KoreaKorean Medicine Life Science Major, Campus of KIOM, University of Science & Technology (UST), Daejeon 34054, KoreaForest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, KoreaForest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, KoreaDepartment of Forest Bioresources, National Institute of Forest Science, Suwon 16631, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaHerbal Medicine Resources Research Center, Korea Institute of Oriental Medicine (KIOM), 111 Geonjae-ro, Naju-si 58245, KoreaHerbal Medicine Resources Research Center, Korea Institute of Oriental Medicine (KIOM), 111 Geonjae-ro, Naju-si 58245, Korea<i>Polygonum multiflorum</i> Thunb. is a perennial plant that belongs to Polygonaceae. Root tissues are the main plant parts used as medicinal herbs in Korean oriental medicine. The <i>P. multiflorum</i> tuber is well known for its medicinal properties in Korean oriental medicine, and it contains a number of useful substances (secondary metabolites of emodin, 2,3,5,4′-tetrahydroxystilbene-2-<i>O</i>-<i>β</i>-<span style="font-variant: small-caps;">d</span>-glucoside (TSG), etc.) that are increasing in demand, as several studies show that they have beneficial effects on the human body. In this study, the production volumes and useful material content differences between cultured <i>P. multiflorum</i> seedlings (culture seedlings: CSs), which had been grown using a tissue culture technique under optimized conditions, and existing varieties in circulation (seed seedlings: SSs) were determined using a long-term field test. The growth characteristics of the underground parts were investigated by harvesting the tuberous roots (medicinal parts) after 1 year, and the results showed that the fresh and dry weights of the CS tubers were higher than those of the SS tubers. However, the SS rootlets had higher fresh and dry weights than the CS rootlets. A liquid chromatography-mass spectrometry component analysis of the <i>P. multiflorum</i> tubers and a Fourier transform near-infrared spectrophotometer analysis of the roots were undertaken. The results showed that the levels of TSG, which is a medicinal substance produced by <i>P. multiflorum</i>, were higher in the CSs than in the SSs, but the differences were not significant. The CS results from this study will inform future studies on the mass production of <i>P. multiflorum</i> in the field because the medicinal area was greater in CSs than in SSs.https://www.mdpi.com/2223-7747/9/10/1280<i>Polygonum multiflorum</i> Thunb.medicinal plantfield productivitybioequivalence validation |
| spellingShingle | Yong-Goo Kim Richard Komakech Dae Hui Jeong Kwonseok Jeon Yunmi Park Tae Kyoung Lee Ki Hyun Kim Byeong Cheol Moon Youngmin Kang Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (<i>Polygonum multiflorum</i>) Developed Using an In Vitro Culture Method Plants <i>Polygonum multiflorum</i> Thunb. medicinal plant field productivity bioequivalence validation |
| title | Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (<i>Polygonum multiflorum</i>) Developed Using an In Vitro Culture Method |
| title_full | Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (<i>Polygonum multiflorum</i>) Developed Using an In Vitro Culture Method |
| title_fullStr | Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (<i>Polygonum multiflorum</i>) Developed Using an In Vitro Culture Method |
| title_full_unstemmed | Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (<i>Polygonum multiflorum</i>) Developed Using an In Vitro Culture Method |
| title_short | Verification of the Field Productivity and Bioequivalence of a Medicinal Plant (<i>Polygonum multiflorum</i>) Developed Using an In Vitro Culture Method |
| title_sort | verification of the field productivity and bioequivalence of a medicinal plant i polygonum multiflorum i developed using an in vitro culture method |
| topic | <i>Polygonum multiflorum</i> Thunb. medicinal plant field productivity bioequivalence validation |
| url | https://www.mdpi.com/2223-7747/9/10/1280 |
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