Exposure to concentrated ambient PM2.5 alters the composition of gut microbiota in a murine model
Abstract Background Exposure to ambient fine particulate matter (PM2.5) correlates with abnormal glucose homeostasis, but the underlying biological mechanism has not been fully understood. The gut microbiota is an emerging crucial player in the homeostatic regulation of glucose metabolism. Few studi...
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | Particle and Fibre Toxicology |
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| Online Access: | http://link.springer.com/article/10.1186/s12989-018-0252-6 |
| _version_ | 1819130995075448832 |
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| author | Wanjun Wang Ji Zhou Minjie Chen Xingke Huang Xiaoyun Xie Weihua Li Qi Cao Haidong Kan Yanyi Xu Zhekang Ying |
| author_facet | Wanjun Wang Ji Zhou Minjie Chen Xingke Huang Xiaoyun Xie Weihua Li Qi Cao Haidong Kan Yanyi Xu Zhekang Ying |
| author_sort | Wanjun Wang |
| collection | DOAJ |
| description | Abstract Background Exposure to ambient fine particulate matter (PM2.5) correlates with abnormal glucose homeostasis, but the underlying biological mechanism has not been fully understood. The gut microbiota is an emerging crucial player in the homeostatic regulation of glucose metabolism. Few studies have investigated its role in the PM2.5 exposure-induced abnormalities in glucose homeostasis. Methods C57Bl/6J mice were exposed to filtered air (FA) or concentrated ambient PM2.5 (CAP) for 12 months using a versatile aerosol concentration enrichment system (VACES) that was modified for long-term whole-body exposures. Their glucose homeostasis and gut microbiota were examined and analysed by correlation and mediation analysis. Results Intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (ITT) showed that CAP exposure markedly impaired their glucose and insulin tolerance. Faecal microbiota analysis demonstrated that the impairment in glucose homeostasis was coincided with decreased faecal bacterial ACE and Chao-1 estimators (the indexes of community richness), while there was no significant change in all faecal fungal alpha diversity estimators. The Pearson’s correlation analyses showed that the bacterial richness estimators were correlated with glucose and insulin tolerance, and the mediation analyses displayed a significant mediation of CAP exposure-induced glucose intolerance by the alteration in the bacterial Chao-1 estimator. LEfSe analyses revealed 24 bacterial and 21 fungal taxa differential between CAP- and FA-exposed animals. Of these, 14 and 20 bacterial taxa were correlated with IPGTT AUC and ITT AUC, respectively, and 5 fungal taxa were correlated with abnormalities in glucose metabolism. Conclusions Chronic exposure to PM2.5 causes gut dysbiosis and may subsequently contribute to the development of abnormalities in glucose metabolism. |
| first_indexed | 2024-12-22T09:08:28Z |
| format | Article |
| id | doaj.art-28ae17d4d72146aea9b376235d93d44a |
| institution | Directory Open Access Journal |
| issn | 1743-8977 |
| language | English |
| last_indexed | 2024-12-22T09:08:28Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Particle and Fibre Toxicology |
| spelling | doaj.art-28ae17d4d72146aea9b376235d93d44a2022-12-21T18:31:32ZengBMCParticle and Fibre Toxicology1743-89772018-04-0115111310.1186/s12989-018-0252-6Exposure to concentrated ambient PM2.5 alters the composition of gut microbiota in a murine modelWanjun Wang0Ji Zhou1Minjie Chen2Xingke Huang3Xiaoyun Xie4Weihua Li5Qi Cao6Haidong Kan7Yanyi Xu8Zhekang Ying9Department of Environmental Health, School of Public Health, Fudan UniversityShanghai Key Laboratory of Meteorology and Health, Shanghai Meteorological ServiceDepartment of Medicine Cardiology Division, School of Medicine, University of MarylandDepartment of Environmental Health, School of Public Health, Fudan UniversityDepartment of Interventional & Vascular Surgery, Shanghai Tenth People’s Hospital, Tongji University School of MedicineReproductive and Developmental Research Institute of Fudan UniversityDepartment of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of MedicineDepartment of Environmental Health, School of Public Health, Fudan UniversityDepartment of Environmental Health, School of Public Health, Fudan UniversityDepartment of Environmental Health, School of Public Health, Fudan UniversityAbstract Background Exposure to ambient fine particulate matter (PM2.5) correlates with abnormal glucose homeostasis, but the underlying biological mechanism has not been fully understood. The gut microbiota is an emerging crucial player in the homeostatic regulation of glucose metabolism. Few studies have investigated its role in the PM2.5 exposure-induced abnormalities in glucose homeostasis. Methods C57Bl/6J mice were exposed to filtered air (FA) or concentrated ambient PM2.5 (CAP) for 12 months using a versatile aerosol concentration enrichment system (VACES) that was modified for long-term whole-body exposures. Their glucose homeostasis and gut microbiota were examined and analysed by correlation and mediation analysis. Results Intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (ITT) showed that CAP exposure markedly impaired their glucose and insulin tolerance. Faecal microbiota analysis demonstrated that the impairment in glucose homeostasis was coincided with decreased faecal bacterial ACE and Chao-1 estimators (the indexes of community richness), while there was no significant change in all faecal fungal alpha diversity estimators. The Pearson’s correlation analyses showed that the bacterial richness estimators were correlated with glucose and insulin tolerance, and the mediation analyses displayed a significant mediation of CAP exposure-induced glucose intolerance by the alteration in the bacterial Chao-1 estimator. LEfSe analyses revealed 24 bacterial and 21 fungal taxa differential between CAP- and FA-exposed animals. Of these, 14 and 20 bacterial taxa were correlated with IPGTT AUC and ITT AUC, respectively, and 5 fungal taxa were correlated with abnormalities in glucose metabolism. Conclusions Chronic exposure to PM2.5 causes gut dysbiosis and may subsequently contribute to the development of abnormalities in glucose metabolism.http://link.springer.com/article/10.1186/s12989-018-0252-6PM2.5Gut microbiotaGlucose homeostaticDiabetes |
| spellingShingle | Wanjun Wang Ji Zhou Minjie Chen Xingke Huang Xiaoyun Xie Weihua Li Qi Cao Haidong Kan Yanyi Xu Zhekang Ying Exposure to concentrated ambient PM2.5 alters the composition of gut microbiota in a murine model Particle and Fibre Toxicology PM2.5 Gut microbiota Glucose homeostatic Diabetes |
| title | Exposure to concentrated ambient PM2.5 alters the composition of gut microbiota in a murine model |
| title_full | Exposure to concentrated ambient PM2.5 alters the composition of gut microbiota in a murine model |
| title_fullStr | Exposure to concentrated ambient PM2.5 alters the composition of gut microbiota in a murine model |
| title_full_unstemmed | Exposure to concentrated ambient PM2.5 alters the composition of gut microbiota in a murine model |
| title_short | Exposure to concentrated ambient PM2.5 alters the composition of gut microbiota in a murine model |
| title_sort | exposure to concentrated ambient pm2 5 alters the composition of gut microbiota in a murine model |
| topic | PM2.5 Gut microbiota Glucose homeostatic Diabetes |
| url | http://link.springer.com/article/10.1186/s12989-018-0252-6 |
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