Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse
Synaptic plasticity involves the modulation of synaptic connections in response to neuronal activity via multiple pathways. One mechanism modulates synaptic transmission by retrograde signals from the post-synapse that influence the probability of vesicle release in the pre-synapse. Despite its impo...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2014-08-01
|
| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/3/9/839 |
| _version_ | 1818656727936008192 |
|---|---|
| author | James M. Halstead Yong Qi Lin Lita Durraine Russell S. Hamilton Graeme Ball Greg G. Neely Hugo J. Bellen Ilan Davis |
| author_facet | James M. Halstead Yong Qi Lin Lita Durraine Russell S. Hamilton Graeme Ball Greg G. Neely Hugo J. Bellen Ilan Davis |
| author_sort | James M. Halstead |
| collection | DOAJ |
| description | Synaptic plasticity involves the modulation of synaptic connections in response to neuronal activity via multiple pathways. One mechanism modulates synaptic transmission by retrograde signals from the post-synapse that influence the probability of vesicle release in the pre-synapse. Despite its importance, very few factors required for the expression of retrograde signals, and proper synaptic transmission, have been identified. Here, we identify the conserved RNA binding protein Syncrip as a new factor that modulates the efficiency of vesicle release from the motoneuron and is required for correct synapse structure. We show that syncrip is required genetically and its protein product is detected only in the muscle and not in the motoneuron itself. This unexpected non-autonomy is at least partly explained by the fact that Syncrip modulates retrograde BMP signals from the muscle back to the motoneuron. We show that Syncrip influences the levels of the Bone Morphogenic Protein ligand Glass Bottom Boat from the post-synapse and regulates the pre-synapse. Our results highlight the RNA-binding protein Syncrip as a novel regulator of synaptic output. Given its known role in regulating translation, we propose that Syncrip is important for maintaining a balance between the strength of presynaptic vesicle release and postsynaptic translation. |
| first_indexed | 2024-12-17T03:30:11Z |
| format | Article |
| id | doaj.art-28aebf7f02234fd8a1701fb25d3ecd30 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-17T03:30:11Z |
| publishDate | 2014-08-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-28aebf7f02234fd8a1701fb25d3ecd302022-12-21T22:05:17ZengThe Company of BiologistsBiology Open2046-63902014-08-013983984910.1242/bio.2014902720149027Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapseJames M. Halstead0Yong Qi Lin1Lita Durraine2Russell S. Hamilton3Graeme Ball4Greg G. Neely5Hugo J. Bellen6Ilan Davis7 Department of Biochemistry, South Parks Road, The University of Oxford, Oxford OX1 3QU, UK Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Department of Neuroscience, Program in Developmental Biology, Neurological Research Institute at Baylor College of Medicine, Houston, TX 77030, USA Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Department of Neuroscience, Program in Developmental Biology, Neurological Research Institute at Baylor College of Medicine, Houston, TX 77030, USA Department of Biochemistry, South Parks Road, The University of Oxford, Oxford OX1 3QU, UK Micron Imaging Facility, Department of Biochemistry, South Parks Road, The University of Oxford, Oxford OX1 3QU, UK Neuroscience Program, Garvan Institute of Medical Research, Darlinghurst, Sydney, NSW 2010, Australia Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Department of Neuroscience, Program in Developmental Biology, Neurological Research Institute at Baylor College of Medicine, Houston, TX 77030, USA Department of Biochemistry, South Parks Road, The University of Oxford, Oxford OX1 3QU, UK Synaptic plasticity involves the modulation of synaptic connections in response to neuronal activity via multiple pathways. One mechanism modulates synaptic transmission by retrograde signals from the post-synapse that influence the probability of vesicle release in the pre-synapse. Despite its importance, very few factors required for the expression of retrograde signals, and proper synaptic transmission, have been identified. Here, we identify the conserved RNA binding protein Syncrip as a new factor that modulates the efficiency of vesicle release from the motoneuron and is required for correct synapse structure. We show that syncrip is required genetically and its protein product is detected only in the muscle and not in the motoneuron itself. This unexpected non-autonomy is at least partly explained by the fact that Syncrip modulates retrograde BMP signals from the muscle back to the motoneuron. We show that Syncrip influences the levels of the Bone Morphogenic Protein ligand Glass Bottom Boat from the post-synapse and regulates the pre-synapse. Our results highlight the RNA-binding protein Syncrip as a novel regulator of synaptic output. Given its known role in regulating translation, we propose that Syncrip is important for maintaining a balance between the strength of presynaptic vesicle release and postsynaptic translation.http://bio.biologists.org/content/3/9/839SyncripDrosophilaLocalized translationSynaptic transmissionmRNA localization neuromuscular junction |
| spellingShingle | James M. Halstead Yong Qi Lin Lita Durraine Russell S. Hamilton Graeme Ball Greg G. Neely Hugo J. Bellen Ilan Davis Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse Biology Open Syncrip Drosophila Localized translation Synaptic transmission mRNA localization neuromuscular junction |
| title | Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse |
| title_full | Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse |
| title_fullStr | Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse |
| title_full_unstemmed | Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse |
| title_short | Syncrip/hnRNP Q influences synaptic transmission and regulates BMP signaling at the Drosophila neuromuscular synapse |
| title_sort | syncrip hnrnp q influences synaptic transmission and regulates bmp signaling at the drosophila neuromuscular synapse |
| topic | Syncrip Drosophila Localized translation Synaptic transmission mRNA localization neuromuscular junction |
| url | http://bio.biologists.org/content/3/9/839 |
| work_keys_str_mv | AT jamesmhalstead syncriphnrnpqinfluencessynaptictransmissionandregulatesbmpsignalingatthedrosophilaneuromuscularsynapse AT yongqilin syncriphnrnpqinfluencessynaptictransmissionandregulatesbmpsignalingatthedrosophilaneuromuscularsynapse AT litadurraine syncriphnrnpqinfluencessynaptictransmissionandregulatesbmpsignalingatthedrosophilaneuromuscularsynapse AT russellshamilton syncriphnrnpqinfluencessynaptictransmissionandregulatesbmpsignalingatthedrosophilaneuromuscularsynapse AT graemeball syncriphnrnpqinfluencessynaptictransmissionandregulatesbmpsignalingatthedrosophilaneuromuscularsynapse AT greggneely syncriphnrnpqinfluencessynaptictransmissionandregulatesbmpsignalingatthedrosophilaneuromuscularsynapse AT hugojbellen syncriphnrnpqinfluencessynaptictransmissionandregulatesbmpsignalingatthedrosophilaneuromuscularsynapse AT ilandavis syncriphnrnpqinfluencessynaptictransmissionandregulatesbmpsignalingatthedrosophilaneuromuscularsynapse |