Correlation analysis of intestinal flora and pathological process of type 2 diabetes mellitus
Objective: To observe the relationship between the different stages of type 2 diabetes mellitus (T2DM) and the intestinal flora and verify its underlying mechanism. Methods: T2DM rats were generated by high-fat diet (HFD) combined with intraperitoneal streptozotocin (STZ) injection. The rats were di...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Journal of Traditional Chinese Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095754822000308 |
| _version_ | 1797756620198903808 |
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| author | Yongcheng An Yuhui Duan Hongyu Dai Chen Wang Lu Shi Changhao He Yinglan Lv Huimin Li Shuhang Dai Baosheng Zhao |
| author_facet | Yongcheng An Yuhui Duan Hongyu Dai Chen Wang Lu Shi Changhao He Yinglan Lv Huimin Li Shuhang Dai Baosheng Zhao |
| author_sort | Yongcheng An |
| collection | DOAJ |
| description | Objective: To observe the relationship between the different stages of type 2 diabetes mellitus (T2DM) and the intestinal flora and verify its underlying mechanism. Methods: T2DM rats were generated by high-fat diet (HFD) combined with intraperitoneal streptozotocin (STZ) injection. The rats were divided into four groups: the control group (fed with normal feed for 1 month), the HFD group (fed with HFD for 1 month), the T2DM group (HFD combined with STZ and blood glucose ≥11.1 mM), and the unformed T2DM model (Un-mod) group (HFD combined with STZ and blood glucose <11.1 mM). Feces were collected, and bacterial communities in the fecal samples were analyzed by 16S rRNA gene sequencing. The content of short-chain fatty acids (SCFAs) in feces was measured by gas chromatography. Western blot and quantitative real-time polymerase chain reaction were used to detect the expression of G protein-coupled receptor 41 (GPR41) and GPR43. Results: At different stages of T2DM, the intestinal flora and SCFAs content of rats were significantly decreased (all P < .05). Our results indicated that g__Prevotella had a significant negative correlation, and g__Ruminococcus_torques_group and g__lachnoclastic had a significant positive correlation with blood glucose. The content of SCFAs, in particular acetate and butyrate, in rat feces of different stages of T2DM were significantly reduced, as well as GPR41 and GPR43 expression. The results in the Un-mod group were similar to the T2DM group, and the expression of GPR41 and GPR43 proteins were significantly higher than those in the T2DM group (both P < .001). Conclusion: The intestinal flora–SCFAs–GPR41/GPR43 network may be important in the development of T2DM. Decreasing blood glucose levels by regulating the intestinal flora may become a new therapeutic strategy for T2DM, which has very important clinical and social values. |
| first_indexed | 2024-03-12T18:04:11Z |
| format | Article |
| id | doaj.art-28b066750042444aa32809a88d5fc8df |
| institution | Directory Open Access Journal |
| issn | 2095-7548 |
| language | English |
| last_indexed | 2024-03-12T18:04:11Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional Chinese Medical Sciences |
| spelling | doaj.art-28b066750042444aa32809a88d5fc8df2023-08-02T09:31:22ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482022-04-0192166180Correlation analysis of intestinal flora and pathological process of type 2 diabetes mellitusYongcheng An0Yuhui Duan1Hongyu Dai2Chen Wang3Lu Shi4Changhao He5Yinglan Lv6Huimin Li7Shuhang Dai8Baosheng Zhao9School of Life Sciences, Beijing University of Chinese Medicine, Beijing, 102488, ChinaDepartment of Pharmacology, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, ChinaDepartment of Pharmacology, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing, 102488, ChinaDepartment of Pharmacology, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, ChinaDepartment of Pharmacology, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, ChinaDepartment of Pharmacology, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, ChinaDepartment of Pharmacology, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, ChinaDepartment of Pharmacology and Toxicology, Jacobs School of Medicine and Biomedical Sciences, State University of New York, Buffalo, New York, NY, USABeijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China; Corresponding author.Objective: To observe the relationship between the different stages of type 2 diabetes mellitus (T2DM) and the intestinal flora and verify its underlying mechanism. Methods: T2DM rats were generated by high-fat diet (HFD) combined with intraperitoneal streptozotocin (STZ) injection. The rats were divided into four groups: the control group (fed with normal feed for 1 month), the HFD group (fed with HFD for 1 month), the T2DM group (HFD combined with STZ and blood glucose ≥11.1 mM), and the unformed T2DM model (Un-mod) group (HFD combined with STZ and blood glucose <11.1 mM). Feces were collected, and bacterial communities in the fecal samples were analyzed by 16S rRNA gene sequencing. The content of short-chain fatty acids (SCFAs) in feces was measured by gas chromatography. Western blot and quantitative real-time polymerase chain reaction were used to detect the expression of G protein-coupled receptor 41 (GPR41) and GPR43. Results: At different stages of T2DM, the intestinal flora and SCFAs content of rats were significantly decreased (all P < .05). Our results indicated that g__Prevotella had a significant negative correlation, and g__Ruminococcus_torques_group and g__lachnoclastic had a significant positive correlation with blood glucose. The content of SCFAs, in particular acetate and butyrate, in rat feces of different stages of T2DM were significantly reduced, as well as GPR41 and GPR43 expression. The results in the Un-mod group were similar to the T2DM group, and the expression of GPR41 and GPR43 proteins were significantly higher than those in the T2DM group (both P < .001). Conclusion: The intestinal flora–SCFAs–GPR41/GPR43 network may be important in the development of T2DM. Decreasing blood glucose levels by regulating the intestinal flora may become a new therapeutic strategy for T2DM, which has very important clinical and social values.http://www.sciencedirect.com/science/article/pii/S2095754822000308Type 2 diabetes mellitusIntestinal flora16S rRNA gene sequencingAnalysis of correlationHigh-fat dietInsulin resistance |
| spellingShingle | Yongcheng An Yuhui Duan Hongyu Dai Chen Wang Lu Shi Changhao He Yinglan Lv Huimin Li Shuhang Dai Baosheng Zhao Correlation analysis of intestinal flora and pathological process of type 2 diabetes mellitus Journal of Traditional Chinese Medical Sciences Type 2 diabetes mellitus Intestinal flora 16S rRNA gene sequencing Analysis of correlation High-fat diet Insulin resistance |
| title | Correlation analysis of intestinal flora and pathological process of type 2 diabetes mellitus |
| title_full | Correlation analysis of intestinal flora and pathological process of type 2 diabetes mellitus |
| title_fullStr | Correlation analysis of intestinal flora and pathological process of type 2 diabetes mellitus |
| title_full_unstemmed | Correlation analysis of intestinal flora and pathological process of type 2 diabetes mellitus |
| title_short | Correlation analysis of intestinal flora and pathological process of type 2 diabetes mellitus |
| title_sort | correlation analysis of intestinal flora and pathological process of type 2 diabetes mellitus |
| topic | Type 2 diabetes mellitus Intestinal flora 16S rRNA gene sequencing Analysis of correlation High-fat diet Insulin resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2095754822000308 |
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