On-line identification of the chemical constituents of Polygoni Multiflori Radix by UHPLC-Q-ToF MS/MS
Introduction: Polygoni Multiflori Radix (PMR) is a type of Chinese herbal medicine with rich chemical composition and pharmacological activity used widely in medicine and food. However, in recent years, there have been increasing numbers of negative reports about its hepatotoxicity. Identification o...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2023.1158717/full |
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| author | Xueting Wang Jianbo Yang Jianbo Yang Xianlong Cheng Ying Wang Huiyu Gao Yunfei Song Feng Wei Shuangcheng Ma |
| author_facet | Xueting Wang Jianbo Yang Jianbo Yang Xianlong Cheng Ying Wang Huiyu Gao Yunfei Song Feng Wei Shuangcheng Ma |
| author_sort | Xueting Wang |
| collection | DOAJ |
| description | Introduction: Polygoni Multiflori Radix (PMR) is a type of Chinese herbal medicine with rich chemical composition and pharmacological activity used widely in medicine and food. However, in recent years, there have been increasing numbers of negative reports about its hepatotoxicity. Identification of its chemical constituents for quality control and safe use is very important.Methods: Three solvents of different polarities (water, 70% ethanol, and 95% ethanol solution) were used to extract the compounds from PMR. Extracts were analyzed and characterized by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-ToF MS/MS) in the negative-ion mode.Results: 152 compounds were detected and identified: 50 anthraquinones, 33 stilbene derivatives, 21 flavonoids, seven naphthalene compounds, and 41 other compounds. Eight other compounds were reported for the first time in the PMR-related literature, and eight other compounds were potentially new compounds.Discussion: This study lays a solid foundation for the screening of toxicity and quality-control indicators of PMR. |
| first_indexed | 2024-04-09T17:01:33Z |
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| id | doaj.art-28b0cc88972940bd84c7f46f5ae2b11e |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-04-09T17:01:33Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-28b0cc88972940bd84c7f46f5ae2b11e2023-04-21T04:34:35ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462023-04-011110.3389/fchem.2023.11587171158717On-line identification of the chemical constituents of Polygoni Multiflori Radix by UHPLC-Q-ToF MS/MSXueting Wang0Jianbo Yang1Jianbo Yang2Xianlong Cheng3Ying Wang4Huiyu Gao5Yunfei Song6Feng Wei7Shuangcheng Ma8Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, ChinaXinjiang Uygur Autonomous Region Drug Inspection and Research Institute NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine (Uyghur) Medicine Urumqi, Urumqi, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, ChinaIntroduction: Polygoni Multiflori Radix (PMR) is a type of Chinese herbal medicine with rich chemical composition and pharmacological activity used widely in medicine and food. However, in recent years, there have been increasing numbers of negative reports about its hepatotoxicity. Identification of its chemical constituents for quality control and safe use is very important.Methods: Three solvents of different polarities (water, 70% ethanol, and 95% ethanol solution) were used to extract the compounds from PMR. Extracts were analyzed and characterized by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-ToF MS/MS) in the negative-ion mode.Results: 152 compounds were detected and identified: 50 anthraquinones, 33 stilbene derivatives, 21 flavonoids, seven naphthalene compounds, and 41 other compounds. Eight other compounds were reported for the first time in the PMR-related literature, and eight other compounds were potentially new compounds.Discussion: This study lays a solid foundation for the screening of toxicity and quality-control indicators of PMR.https://www.frontiersin.org/articles/10.3389/fchem.2023.1158717/fullPolygoni Multiflori Radixmass spectrometryhepatotoxicityidentificationchemical components |
| spellingShingle | Xueting Wang Jianbo Yang Jianbo Yang Xianlong Cheng Ying Wang Huiyu Gao Yunfei Song Feng Wei Shuangcheng Ma On-line identification of the chemical constituents of Polygoni Multiflori Radix by UHPLC-Q-ToF MS/MS Frontiers in Chemistry Polygoni Multiflori Radix mass spectrometry hepatotoxicity identification chemical components |
| title | On-line identification of the chemical constituents of Polygoni Multiflori Radix by UHPLC-Q-ToF MS/MS |
| title_full | On-line identification of the chemical constituents of Polygoni Multiflori Radix by UHPLC-Q-ToF MS/MS |
| title_fullStr | On-line identification of the chemical constituents of Polygoni Multiflori Radix by UHPLC-Q-ToF MS/MS |
| title_full_unstemmed | On-line identification of the chemical constituents of Polygoni Multiflori Radix by UHPLC-Q-ToF MS/MS |
| title_short | On-line identification of the chemical constituents of Polygoni Multiflori Radix by UHPLC-Q-ToF MS/MS |
| title_sort | on line identification of the chemical constituents of polygoni multiflori radix by uhplc q tof ms ms |
| topic | Polygoni Multiflori Radix mass spectrometry hepatotoxicity identification chemical components |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2023.1158717/full |
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