Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates
Abstract Yak butter is one of the most important foods for the Tibetan people. Of note, its production yields waste yak milk as a by-product. In this work, waste yak milk protein hydrolysates made via Pepsin hydrolysis were shown to have antimicrobial activity. Furthermore, an innovative method of m...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-12-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13568-017-0497-8 |
| _version_ | 1819019897617776640 |
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| author | Jinjin Pei Hai Jiang Xinsheng Li Wengang Jin Yanduo Tao |
| author_facet | Jinjin Pei Hai Jiang Xinsheng Li Wengang Jin Yanduo Tao |
| author_sort | Jinjin Pei |
| collection | DOAJ |
| description | Abstract Yak butter is one of the most important foods for the Tibetan people. Of note, its production yields waste yak milk as a by-product. In this work, waste yak milk protein hydrolysates made via Pepsin hydrolysis were shown to have antimicrobial activity. Furthermore, an innovative method of magnetic liposome adsorption combined with reversed-phase high performance liquid chromatography (RP-HPLC) was developed to screen for and purify the antimicrobial peptides. Two antimicrobial peptides were obtained and their amino acid sequences were determined by N-sequencing, namely Arg-Val-Met-Phe-Lys-Trp-Ala and Lys-Val-Ile-Ser-Met-Ile. The antimicrobial activity spectra of Arg-Val-Met-Phe-Lys-Trp-Ala included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua, Escherichia coli, Enterobacter cloacae and Salmonella paratyphi, while the Lys-Val-Ile-Ser-Met-Ile peptide shows not only bacterial growth inhibition but also of fungi. Haemolytic testing suggested that these two antimicrobial peptides could be considered to have no haemolytic effect at their minimum inhibitory concentrations (MICs). |
| first_indexed | 2024-12-21T03:42:37Z |
| format | Article |
| id | doaj.art-28b0ced7b9204a8dbe19ae34a5668763 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-21T03:42:37Z |
| publishDate | 2017-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-28b0ced7b9204a8dbe19ae34a56687632022-12-21T19:17:10ZengSpringerOpenAMB Express2191-08552017-12-01711610.1186/s13568-017-0497-8Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysatesJinjin Pei0Hai Jiang1Xinsheng Li2Wengang Jin3Yanduo Tao4Shaanxi Key Laboratory of Biology and Bioresources, College of Bioscience and Bioengineering, Shaanxi University of TechnologyShaanxi Key Laboratory of Biology and Bioresources, College of Bioscience and Bioengineering, Shaanxi University of TechnologyShaanxi Key Laboratory of Biology and Bioresources, College of Bioscience and Bioengineering, Shaanxi University of TechnologyShaanxi Key Laboratory of Biology and Bioresources, College of Bioscience and Bioengineering, Shaanxi University of TechnologyKey Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences XiningAbstract Yak butter is one of the most important foods for the Tibetan people. Of note, its production yields waste yak milk as a by-product. In this work, waste yak milk protein hydrolysates made via Pepsin hydrolysis were shown to have antimicrobial activity. Furthermore, an innovative method of magnetic liposome adsorption combined with reversed-phase high performance liquid chromatography (RP-HPLC) was developed to screen for and purify the antimicrobial peptides. Two antimicrobial peptides were obtained and their amino acid sequences were determined by N-sequencing, namely Arg-Val-Met-Phe-Lys-Trp-Ala and Lys-Val-Ile-Ser-Met-Ile. The antimicrobial activity spectra of Arg-Val-Met-Phe-Lys-Trp-Ala included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua, Escherichia coli, Enterobacter cloacae and Salmonella paratyphi, while the Lys-Val-Ile-Ser-Met-Ile peptide shows not only bacterial growth inhibition but also of fungi. Haemolytic testing suggested that these two antimicrobial peptides could be considered to have no haemolytic effect at their minimum inhibitory concentrations (MICs).http://link.springer.com/article/10.1186/s13568-017-0497-8Antimicrobial peptidesYak milkHydrolysatesIsolation |
| spellingShingle | Jinjin Pei Hai Jiang Xinsheng Li Wengang Jin Yanduo Tao Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates AMB Express Antimicrobial peptides Yak milk Hydrolysates Isolation |
| title | Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates |
| title_full | Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates |
| title_fullStr | Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates |
| title_full_unstemmed | Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates |
| title_short | Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates |
| title_sort | antimicrobial peptides sourced from post butter processing waste yak milk protein hydrolysates |
| topic | Antimicrobial peptides Yak milk Hydrolysates Isolation |
| url | http://link.springer.com/article/10.1186/s13568-017-0497-8 |
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