Biochemical surface functionalization of iron oxide for efficient biomarker detector: A new visions of nano-bio interactions
The utilization of nanomaterials and their compatibility with biological systems have become major concern for the researcher. In this particular study, Fe2O3 nanoparticles (NPs) were synthesized using both green chemistry and chemical-reduction precipitation methods. The biological Fe2O3 NPs were w...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Applied Surface Science Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666523923001204 |
| _version_ | 1797389392865656832 |
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| author | Murtaza Hasan Huang Xue Ayesha Zafar Ain ul Haq Tuba Tariq Muhammad Mahmood Ahmad Shahbaz Gul Hassan Hafiz Umer Javed Xuecheng Chen Xugang Shu |
| author_facet | Murtaza Hasan Huang Xue Ayesha Zafar Ain ul Haq Tuba Tariq Muhammad Mahmood Ahmad Shahbaz Gul Hassan Hafiz Umer Javed Xuecheng Chen Xugang Shu |
| author_sort | Murtaza Hasan |
| collection | DOAJ |
| description | The utilization of nanomaterials and their compatibility with biological systems have become major concern for the researcher. In this particular study, Fe2O3 nanoparticles (NPs) were synthesized using both green chemistry and chemical-reduction precipitation methods. The biological Fe2O3 NPs were well characterized and found to have a size of 23.01 nm, while the chemical Fe2O3 NPs had a size of 40.80 nm. Stable Fe2O3 NPs were allowed to interact with the serum of cancer patients for different time intervals (24 h, 48 h, and 72 h). The chemical Fe2O3 NPs yielded 41 differentially expressed proteins, whereas the biological Fe2O3 NPs yielded 103. Through proteomics algorithms, a deeper understanding of the obtained proteins revealed the presence of domains and motifs that are primarily associated with the onset of oncogenesis. Notably, key oncogenic and onco-histone protein domains such as PLEC, DEXDc, H2A, and H2B were specifically and uniquely present in the biologically prepared Fe2O3 NP. In conclusion, the efficient use of biological Fe2O3 NPs demonstrated significant potential in adsorbing proteins and peptides, which could be utilized in diagnostic and therapeutic strategies. |
| first_indexed | 2024-03-08T22:55:13Z |
| format | Article |
| id | doaj.art-28b4a77db5da4f2580b5730fa0161acb |
| institution | Directory Open Access Journal |
| issn | 2666-5239 |
| language | English |
| last_indexed | 2024-03-08T22:55:13Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Applied Surface Science Advances |
| spelling | doaj.art-28b4a77db5da4f2580b5730fa0161acb2023-12-16T06:09:27ZengElsevierApplied Surface Science Advances2666-52392023-12-0118100486Biochemical surface functionalization of iron oxide for efficient biomarker detector: A new visions of nano-bio interactionsMurtaza Hasan0Huang Xue1Ayesha Zafar2Ain ul Haq3Tuba Tariq4Muhammad Mahmood Ahmad5Shahbaz Gul Hassan6Hafiz Umer Javed7Xuecheng Chen8Xugang Shu9School of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, PR China; Faculty of Chemical and Biological Science, Department of Biotechnology, The Islamia University of Bahawalpur, 63100, Pakistan; Corresponding authors.School of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, PR ChinaSchool of Engineering, Royal Melbourne Institute of Technology (RMIT) University Melbourne, 124 La Trobe Street, Melbourne, VIC 3001, Australia; Faculty of Chemical and Biological Science, Department of Biotechnology, The Islamia University of Bahawalpur, 63100, PakistanFaculty of Chemical and Biological Science, Department of Biotechnology, The Islamia University of Bahawalpur, 63100, PakistanFaculty of Chemical and Biological Science, Department of Biotechnology, The Islamia University of Bahawalpur, 63100, PakistanFaculty of Chemical and Biological Science, Department of Bioinformatics, The Islamia University of Bahawalpur, 63100, PakistanCollege of Information Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaSchool of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, PR ChinaFaculty of Chemical Technology and Engineering, West Pomeranian University of Technology, Piastów Ave. 42, 71-065 Szczecin, PolandSchool of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, PR China; Corresponding authors.The utilization of nanomaterials and their compatibility with biological systems have become major concern for the researcher. In this particular study, Fe2O3 nanoparticles (NPs) were synthesized using both green chemistry and chemical-reduction precipitation methods. The biological Fe2O3 NPs were well characterized and found to have a size of 23.01 nm, while the chemical Fe2O3 NPs had a size of 40.80 nm. Stable Fe2O3 NPs were allowed to interact with the serum of cancer patients for different time intervals (24 h, 48 h, and 72 h). The chemical Fe2O3 NPs yielded 41 differentially expressed proteins, whereas the biological Fe2O3 NPs yielded 103. Through proteomics algorithms, a deeper understanding of the obtained proteins revealed the presence of domains and motifs that are primarily associated with the onset of oncogenesis. Notably, key oncogenic and onco-histone protein domains such as PLEC, DEXDc, H2A, and H2B were specifically and uniquely present in the biologically prepared Fe2O3 NP. In conclusion, the efficient use of biological Fe2O3 NPs demonstrated significant potential in adsorbing proteins and peptides, which could be utilized in diagnostic and therapeutic strategies.http://www.sciencedirect.com/science/article/pii/S2666523923001204NanoscaleProtein adsorptionDiagnosticsBiomarkerNanomaterials |
| spellingShingle | Murtaza Hasan Huang Xue Ayesha Zafar Ain ul Haq Tuba Tariq Muhammad Mahmood Ahmad Shahbaz Gul Hassan Hafiz Umer Javed Xuecheng Chen Xugang Shu Biochemical surface functionalization of iron oxide for efficient biomarker detector: A new visions of nano-bio interactions Applied Surface Science Advances Nanoscale Protein adsorption Diagnostics Biomarker Nanomaterials |
| title | Biochemical surface functionalization of iron oxide for efficient biomarker detector: A new visions of nano-bio interactions |
| title_full | Biochemical surface functionalization of iron oxide for efficient biomarker detector: A new visions of nano-bio interactions |
| title_fullStr | Biochemical surface functionalization of iron oxide for efficient biomarker detector: A new visions of nano-bio interactions |
| title_full_unstemmed | Biochemical surface functionalization of iron oxide for efficient biomarker detector: A new visions of nano-bio interactions |
| title_short | Biochemical surface functionalization of iron oxide for efficient biomarker detector: A new visions of nano-bio interactions |
| title_sort | biochemical surface functionalization of iron oxide for efficient biomarker detector a new visions of nano bio interactions |
| topic | Nanoscale Protein adsorption Diagnostics Biomarker Nanomaterials |
| url | http://www.sciencedirect.com/science/article/pii/S2666523923001204 |
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