Genome-Wide Identification of Fatty Acyl-CoA Reductase (FAR) Genes in <i>Dendrobium catenatum</i> and Their Response to Drought Stress
<i>Dendrobium catenatum</i> is a high-value medicinal plant that is predominantly found in high mountain areas, thriving amidst cliffs and rock crevices. However, its wild resources face constant threats from adverse environmental conditions, especially drought stress. Fatty acyl-CoA red...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/9/982 |
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| author | Yutong Ren Peng Wang Tingting Zhang Wen Liu Yujuan Wang Jun Dai Yang Zhou |
| author_facet | Yutong Ren Peng Wang Tingting Zhang Wen Liu Yujuan Wang Jun Dai Yang Zhou |
| author_sort | Yutong Ren |
| collection | DOAJ |
| description | <i>Dendrobium catenatum</i> is a high-value medicinal plant that is predominantly found in high mountain areas, thriving amidst cliffs and rock crevices. However, its wild resources face constant threats from adverse environmental conditions, especially drought stress. Fatty acyl-CoA reductase (FAR) is crucial in plant drought resistance, but there is a lack of research on <i>FAR</i> genes in <i>D. catenatum</i>. In this study, the FAR family genes were identified from the <i>D. catenatum</i> genome. Their genomic characteristics were investigated using bioinformatics techniques, and their expression patterns in different tissues and under 20% PEG8000 conditions mimicking drought stress were analyzed using quantitative real-time RT-PCR (RT-qPCR). Seven <i>DcFAR</i> genes were identified from the <i>D. catenatum</i> genome. The encoded amino acids range between 377 and 587 aa, with molecular weights between 43.41 and 66.15 kD and isoelectric points between 5.55 and 9.02. Based on the phylogenetic relationships, the FAR family genes were categorized into three subgroups, each with similar conserved sequences and gene structures. The <i>cis</i>-acting elements of the promoter regions were assessed, and the results reveal that the <i>DcFAR</i> upstream promoter region contains multiple stress-related elements, suggesting its potential involvement in abiotic stress responses. The RT-qPCR results show distinct expression patterns of <i>DcFAR</i> genes in various plant tissues. It was observed that the expression of most <i>DcFAR</i> genes was upregulated under drought stress. Among them, the expression levels of <i>DcFAR2</i>, <i>DcFAR3</i>, <i>DcFAR5</i>, and <i>DcFAR7</i> genes under drought stress were 544-, 193-, 183-, and 214-fold higher compared to the control, respectively. These results indicate that DcFAR2/3/5/7 might play significant roles in <i>D. catenatum</i> drought tolerance. This research offers insight into the function of DcFAR genes and provides theoretical support for breeding drought-resistant <i>D. catenatum</i> varieties. |
| first_indexed | 2024-03-10T22:41:58Z |
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| id | doaj.art-28b5531b3155472f85e88ec734cf1fb6 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T22:41:58Z |
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| spelling | doaj.art-28b5531b3155472f85e88ec734cf1fb62023-11-19T10:58:33ZengMDPI AGHorticulturae2311-75242023-08-019998210.3390/horticulturae9090982Genome-Wide Identification of Fatty Acyl-CoA Reductase (FAR) Genes in <i>Dendrobium catenatum</i> and Their Response to Drought StressYutong Ren0Peng Wang1Tingting Zhang2Wen Liu3Yujuan Wang4Jun Dai5Yang Zhou6Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Tropical Agriculture and Forestry (School of Agricultural and Rural Affairs, School of Rural Revitalization), Hainan University, Haikou 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Tropical Agriculture and Forestry (School of Agricultural and Rural Affairs, School of Rural Revitalization), Hainan University, Haikou 570228, ChinaXiangyang Academy of Agricultural Sciences, Xiangyang 441057, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Tropical Agriculture and Forestry (School of Agricultural and Rural Affairs, School of Rural Revitalization), Hainan University, Haikou 570228, ChinaAnhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, West Anhui University, Lu’an 237012, ChinaAnhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, West Anhui University, Lu’an 237012, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Tropical Agriculture and Forestry (School of Agricultural and Rural Affairs, School of Rural Revitalization), Hainan University, Haikou 570228, China<i>Dendrobium catenatum</i> is a high-value medicinal plant that is predominantly found in high mountain areas, thriving amidst cliffs and rock crevices. However, its wild resources face constant threats from adverse environmental conditions, especially drought stress. Fatty acyl-CoA reductase (FAR) is crucial in plant drought resistance, but there is a lack of research on <i>FAR</i> genes in <i>D. catenatum</i>. In this study, the FAR family genes were identified from the <i>D. catenatum</i> genome. Their genomic characteristics were investigated using bioinformatics techniques, and their expression patterns in different tissues and under 20% PEG8000 conditions mimicking drought stress were analyzed using quantitative real-time RT-PCR (RT-qPCR). Seven <i>DcFAR</i> genes were identified from the <i>D. catenatum</i> genome. The encoded amino acids range between 377 and 587 aa, with molecular weights between 43.41 and 66.15 kD and isoelectric points between 5.55 and 9.02. Based on the phylogenetic relationships, the FAR family genes were categorized into three subgroups, each with similar conserved sequences and gene structures. The <i>cis</i>-acting elements of the promoter regions were assessed, and the results reveal that the <i>DcFAR</i> upstream promoter region contains multiple stress-related elements, suggesting its potential involvement in abiotic stress responses. The RT-qPCR results show distinct expression patterns of <i>DcFAR</i> genes in various plant tissues. It was observed that the expression of most <i>DcFAR</i> genes was upregulated under drought stress. Among them, the expression levels of <i>DcFAR2</i>, <i>DcFAR3</i>, <i>DcFAR5</i>, and <i>DcFAR7</i> genes under drought stress were 544-, 193-, 183-, and 214-fold higher compared to the control, respectively. These results indicate that DcFAR2/3/5/7 might play significant roles in <i>D. catenatum</i> drought tolerance. This research offers insight into the function of DcFAR genes and provides theoretical support for breeding drought-resistant <i>D. catenatum</i> varieties.https://www.mdpi.com/2311-7524/9/9/982<i>Dendrobium catenatum</i>FAR gene familyexpression patternsdrought stress |
| spellingShingle | Yutong Ren Peng Wang Tingting Zhang Wen Liu Yujuan Wang Jun Dai Yang Zhou Genome-Wide Identification of Fatty Acyl-CoA Reductase (FAR) Genes in <i>Dendrobium catenatum</i> and Their Response to Drought Stress Horticulturae <i>Dendrobium catenatum</i> FAR gene family expression patterns drought stress |
| title | Genome-Wide Identification of Fatty Acyl-CoA Reductase (FAR) Genes in <i>Dendrobium catenatum</i> and Their Response to Drought Stress |
| title_full | Genome-Wide Identification of Fatty Acyl-CoA Reductase (FAR) Genes in <i>Dendrobium catenatum</i> and Their Response to Drought Stress |
| title_fullStr | Genome-Wide Identification of Fatty Acyl-CoA Reductase (FAR) Genes in <i>Dendrobium catenatum</i> and Their Response to Drought Stress |
| title_full_unstemmed | Genome-Wide Identification of Fatty Acyl-CoA Reductase (FAR) Genes in <i>Dendrobium catenatum</i> and Their Response to Drought Stress |
| title_short | Genome-Wide Identification of Fatty Acyl-CoA Reductase (FAR) Genes in <i>Dendrobium catenatum</i> and Their Response to Drought Stress |
| title_sort | genome wide identification of fatty acyl coa reductase far genes in i dendrobium catenatum i and their response to drought stress |
| topic | <i>Dendrobium catenatum</i> FAR gene family expression patterns drought stress |
| url | https://www.mdpi.com/2311-7524/9/9/982 |
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