Arp2/3 Complex Is Required for Auxin-Driven Cell Expansion Through Regulation of Auxin Transporter Homeostasis
The Arp2/3 complex is an actin nucleator shown to be required throughout plant morphogenesis, contributing to processes such as cell expansion, tissue differentiation or cell wall assembly. A recent publication demonstrated that plants lacking functional Arp2/3 complex also present defects in auxin...
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| Format: | Article |
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Frontiers Media S.A.
2020-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00486/full |
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| author | Judith García-González Štépánka Kebrlová Matěj Semerák Jozef Lacek Jozef Lacek Innu Kotannal Baby Jan Petrášek Kateřina Schwarzerová |
| author_facet | Judith García-González Štépánka Kebrlová Matěj Semerák Jozef Lacek Jozef Lacek Innu Kotannal Baby Jan Petrášek Kateřina Schwarzerová |
| author_sort | Judith García-González |
| collection | DOAJ |
| description | The Arp2/3 complex is an actin nucleator shown to be required throughout plant morphogenesis, contributing to processes such as cell expansion, tissue differentiation or cell wall assembly. A recent publication demonstrated that plants lacking functional Arp2/3 complex also present defects in auxin distribution and transport. This work shows that Arp2/3 complex subunits are predominantly expressed in the provasculature, although other plant tissues also show promoter activity (e.g., cotyledons, apical meristems, or root tip). Moreover, auxin can trigger subunit expression, indicating a role of this phytohormone in mediating the complex activity. Further investigation of the functional interaction between Arp2/3 complex and auxin signaling also reveals their cooperation in determining pavement cell shape, presumably through the role of Arp2/3 complex in the correct auxin carrier trafficking. Young seedlings of arpc5 mutants show increased auxin-triggered proteasomal degradation of DII-VENUS and altered PIN3 distribution, with higher levels of the protein in the vacuole. Closer observation of vacuolar morphology revealed the presence of a more fragmented vacuolar compartment when Arp2/3 function is abolished, hinting a generalized role of Arp2/3 complex in endomembrane function and protein trafficking. |
| first_indexed | 2024-12-10T05:30:42Z |
| format | Article |
| id | doaj.art-28b6618f6f1c451aab9d77e64d7940a6 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T05:30:42Z |
| publishDate | 2020-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-28b6618f6f1c451aab9d77e64d7940a62022-12-22T02:00:34ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-04-011110.3389/fpls.2020.00486526470Arp2/3 Complex Is Required for Auxin-Driven Cell Expansion Through Regulation of Auxin Transporter HomeostasisJudith García-González0Štépánka Kebrlová1Matěj Semerák2Jozef Lacek3Jozef Lacek4Innu Kotannal Baby5Jan Petrášek6Kateřina Schwarzerová7Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, CzechiaDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Prague, CzechiaDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Prague, CzechiaDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Prague, CzechiaInstitute of Experimental Botany, Czech Academy of Sciences, Prague, CzechiaDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Prague, CzechiaDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Prague, CzechiaDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Prague, CzechiaThe Arp2/3 complex is an actin nucleator shown to be required throughout plant morphogenesis, contributing to processes such as cell expansion, tissue differentiation or cell wall assembly. A recent publication demonstrated that plants lacking functional Arp2/3 complex also present defects in auxin distribution and transport. This work shows that Arp2/3 complex subunits are predominantly expressed in the provasculature, although other plant tissues also show promoter activity (e.g., cotyledons, apical meristems, or root tip). Moreover, auxin can trigger subunit expression, indicating a role of this phytohormone in mediating the complex activity. Further investigation of the functional interaction between Arp2/3 complex and auxin signaling also reveals their cooperation in determining pavement cell shape, presumably through the role of Arp2/3 complex in the correct auxin carrier trafficking. Young seedlings of arpc5 mutants show increased auxin-triggered proteasomal degradation of DII-VENUS and altered PIN3 distribution, with higher levels of the protein in the vacuole. Closer observation of vacuolar morphology revealed the presence of a more fragmented vacuolar compartment when Arp2/3 function is abolished, hinting a generalized role of Arp2/3 complex in endomembrane function and protein trafficking.https://www.frontiersin.org/article/10.3389/fpls.2020.00486/fullactincytoskeletonauxincell expansionArp2/3 complex |
| spellingShingle | Judith García-González Štépánka Kebrlová Matěj Semerák Jozef Lacek Jozef Lacek Innu Kotannal Baby Jan Petrášek Kateřina Schwarzerová Arp2/3 Complex Is Required for Auxin-Driven Cell Expansion Through Regulation of Auxin Transporter Homeostasis Frontiers in Plant Science actin cytoskeleton auxin cell expansion Arp2/3 complex |
| title | Arp2/3 Complex Is Required for Auxin-Driven Cell Expansion Through Regulation of Auxin Transporter Homeostasis |
| title_full | Arp2/3 Complex Is Required for Auxin-Driven Cell Expansion Through Regulation of Auxin Transporter Homeostasis |
| title_fullStr | Arp2/3 Complex Is Required for Auxin-Driven Cell Expansion Through Regulation of Auxin Transporter Homeostasis |
| title_full_unstemmed | Arp2/3 Complex Is Required for Auxin-Driven Cell Expansion Through Regulation of Auxin Transporter Homeostasis |
| title_short | Arp2/3 Complex Is Required for Auxin-Driven Cell Expansion Through Regulation of Auxin Transporter Homeostasis |
| title_sort | arp2 3 complex is required for auxin driven cell expansion through regulation of auxin transporter homeostasis |
| topic | actin cytoskeleton auxin cell expansion Arp2/3 complex |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00486/full |
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