Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient Density
This study was conducted to investigate the impacts of dietary energy and protein on rumen bacterial composition and ruminal metabolites. A total of 12 ruminal samples were collected from Shaanbei white cashmere goats which were divided into two groups, including high-energy and high-protein (Group...
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MDPI AG
2020-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/7/1193 |
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| author | Yaoyue Wang Peng Tang Yafei Xiao Jianming Liu Yulin Chen Yuxin Yang |
| author_facet | Yaoyue Wang Peng Tang Yafei Xiao Jianming Liu Yulin Chen Yuxin Yang |
| author_sort | Yaoyue Wang |
| collection | DOAJ |
| description | This study was conducted to investigate the impacts of dietary energy and protein on rumen bacterial composition and ruminal metabolites. A total of 12 ruminal samples were collected from Shaanbei white cashmere goats which were divided into two groups, including high-energy and high-protein (Group H; crude protein, CP: 9.37% in dry matter; metabolic energy, ME: 9.24 MJ/kg) and control (Group C; CP: 8.73%; ME: 8.60 MJ/kg) groups. Thereby, 16S rRNA gene sequencing and a quantitative polymerase chain reaction were performed to identify the rumen bacterial community. Metabolomics analysis was done to investigate the rumen metabolites and the related metabolic pathways in Groups C and H. The high-energy and high-protein diets increased the relative abundance of phylum Bacteroidetes and genera <i>Prevotella</i>_1 and <i>Succiniclasticum</i>, while decreasing the number of Proteobacteria (<i>p</i> < 0.05). The dominant differential metabolites were amino acids, peptides, and analogs. Tyrosine metabolism played an important role among the nine main metabolic pathways. Correlation analysis revealed that both <i>Prevotella</i>_1 (<i>r</i> = 0.608, <i>p</i> < 0.05) and <i>Ruminococcus</i>_2 (<i>r</i> = 0.613, <i>p</i> < 0.05) showed a positive correlation with catechol. Our findings revealed that the diets with high energy and protein levels in Group H significantly altered the composition of ruminal bacteria and metabolites, which can help to improve the dietary energy and protein use efficiency in goats. |
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| spelling | doaj.art-28b7296cd81e47d7b280311a2b99c5432023-11-20T06:43:39ZengMDPI AGAnimals2076-26152020-07-01107119310.3390/ani10071193Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient DensityYaoyue Wang0Peng Tang1Yafei Xiao2Jianming Liu3Yulin Chen4Yuxin Yang5College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaThis study was conducted to investigate the impacts of dietary energy and protein on rumen bacterial composition and ruminal metabolites. A total of 12 ruminal samples were collected from Shaanbei white cashmere goats which were divided into two groups, including high-energy and high-protein (Group H; crude protein, CP: 9.37% in dry matter; metabolic energy, ME: 9.24 MJ/kg) and control (Group C; CP: 8.73%; ME: 8.60 MJ/kg) groups. Thereby, 16S rRNA gene sequencing and a quantitative polymerase chain reaction were performed to identify the rumen bacterial community. Metabolomics analysis was done to investigate the rumen metabolites and the related metabolic pathways in Groups C and H. The high-energy and high-protein diets increased the relative abundance of phylum Bacteroidetes and genera <i>Prevotella</i>_1 and <i>Succiniclasticum</i>, while decreasing the number of Proteobacteria (<i>p</i> < 0.05). The dominant differential metabolites were amino acids, peptides, and analogs. Tyrosine metabolism played an important role among the nine main metabolic pathways. Correlation analysis revealed that both <i>Prevotella</i>_1 (<i>r</i> = 0.608, <i>p</i> < 0.05) and <i>Ruminococcus</i>_2 (<i>r</i> = 0.613, <i>p</i> < 0.05) showed a positive correlation with catechol. Our findings revealed that the diets with high energy and protein levels in Group H significantly altered the composition of ruminal bacteria and metabolites, which can help to improve the dietary energy and protein use efficiency in goats.https://www.mdpi.com/2076-2615/10/7/1193dietary energy and proteinmetabolomicsrumen bacterial compositionShaanbei white cashmere goats16S rRNA gene sequencing |
| spellingShingle | Yaoyue Wang Peng Tang Yafei Xiao Jianming Liu Yulin Chen Yuxin Yang Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient Density Animals dietary energy and protein metabolomics rumen bacterial composition Shaanbei white cashmere goats 16S rRNA gene sequencing |
| title | Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient Density |
| title_full | Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient Density |
| title_fullStr | Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient Density |
| title_full_unstemmed | Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient Density |
| title_short | Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient Density |
| title_sort | alterations in rumen bacterial community and metabolome characteristics of cashmere goats in response to dietary nutrient density |
| topic | dietary energy and protein metabolomics rumen bacterial composition Shaanbei white cashmere goats 16S rRNA gene sequencing |
| url | https://www.mdpi.com/2076-2615/10/7/1193 |
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