M6A regulator methylation patterns and characteristics of immunity in acute ST-segment elevation myocardial infarction
Abstract M6A methylation is the most prevalent and abundant RNA modification in mammals. Although there are many studies on the regulatory role of m6A methylation in the immune response, the m6A regulators in the pathogenesis of acute ST-segment elevation myocardial infarction (STEMI) remain unclear...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-09-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-42959-5 |
| _version_ | 1797576678929596416 |
|---|---|
| author | Jingqi Yang Qing Shangguan Guobo Xie Ming Yang Guotai Sheng |
| author_facet | Jingqi Yang Qing Shangguan Guobo Xie Ming Yang Guotai Sheng |
| author_sort | Jingqi Yang |
| collection | DOAJ |
| description | Abstract M6A methylation is the most prevalent and abundant RNA modification in mammals. Although there are many studies on the regulatory role of m6A methylation in the immune response, the m6A regulators in the pathogenesis of acute ST-segment elevation myocardial infarction (STEMI) remain unclear. We comprehensively analysed the role of m6A regulators in STEMI and built a predictive model, revealing the relationship between m6A methylations and the immune microenvironment. Differential analysis revealed that 18 of 24 m6A regulators were significantly differentially expressed, and there were substantial interactions between the m6A regulator. Then, we established a classifier and nomogram model based on 6 m6A regulators, which can easily distinguish the STEMI and control samples. Finally, two distinct m6A subtypes were obtained and significantly differentially expressed in terms of infiltrating immunocyte abundance, immune reaction activity and human leukocyte antigen genes. Three hub m6A phenotype related genes (RAC2, RELA, and WAS) in the midnightblue module were identified by weighted gene coexpression network analysis, and were associated with immunity. These findings suggest that m6A modification and the immune microenvironment play a key role in the pathogenesis of STEMI. |
| first_indexed | 2024-03-10T21:56:16Z |
| format | Article |
| id | doaj.art-28b78c33f7744a0aac6f73f5e3742b7a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T21:56:16Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-28b78c33f7744a0aac6f73f5e3742b7a2023-11-19T13:06:19ZengNature PortfolioScientific Reports2045-23222023-09-0113111410.1038/s41598-023-42959-5M6A regulator methylation patterns and characteristics of immunity in acute ST-segment elevation myocardial infarctionJingqi Yang0Qing Shangguan1Guobo Xie2Ming Yang3Guotai Sheng4Department of Cardiovascular Medicine, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical CollegeDepartment of Cardiovascular Medicine, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical CollegeDepartment of Cardiovascular Medicine, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical CollegeDepartment of Cardiovascular Medicine, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical CollegeDepartment of Cardiovascular Medicine, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical CollegeAbstract M6A methylation is the most prevalent and abundant RNA modification in mammals. Although there are many studies on the regulatory role of m6A methylation in the immune response, the m6A regulators in the pathogenesis of acute ST-segment elevation myocardial infarction (STEMI) remain unclear. We comprehensively analysed the role of m6A regulators in STEMI and built a predictive model, revealing the relationship between m6A methylations and the immune microenvironment. Differential analysis revealed that 18 of 24 m6A regulators were significantly differentially expressed, and there were substantial interactions between the m6A regulator. Then, we established a classifier and nomogram model based on 6 m6A regulators, which can easily distinguish the STEMI and control samples. Finally, two distinct m6A subtypes were obtained and significantly differentially expressed in terms of infiltrating immunocyte abundance, immune reaction activity and human leukocyte antigen genes. Three hub m6A phenotype related genes (RAC2, RELA, and WAS) in the midnightblue module were identified by weighted gene coexpression network analysis, and were associated with immunity. These findings suggest that m6A modification and the immune microenvironment play a key role in the pathogenesis of STEMI.https://doi.org/10.1038/s41598-023-42959-5 |
| spellingShingle | Jingqi Yang Qing Shangguan Guobo Xie Ming Yang Guotai Sheng M6A regulator methylation patterns and characteristics of immunity in acute ST-segment elevation myocardial infarction Scientific Reports |
| title | M6A regulator methylation patterns and characteristics of immunity in acute ST-segment elevation myocardial infarction |
| title_full | M6A regulator methylation patterns and characteristics of immunity in acute ST-segment elevation myocardial infarction |
| title_fullStr | M6A regulator methylation patterns and characteristics of immunity in acute ST-segment elevation myocardial infarction |
| title_full_unstemmed | M6A regulator methylation patterns and characteristics of immunity in acute ST-segment elevation myocardial infarction |
| title_short | M6A regulator methylation patterns and characteristics of immunity in acute ST-segment elevation myocardial infarction |
| title_sort | m6a regulator methylation patterns and characteristics of immunity in acute st segment elevation myocardial infarction |
| url | https://doi.org/10.1038/s41598-023-42959-5 |
| work_keys_str_mv | AT jingqiyang m6aregulatormethylationpatternsandcharacteristicsofimmunityinacutestsegmentelevationmyocardialinfarction AT qingshangguan m6aregulatormethylationpatternsandcharacteristicsofimmunityinacutestsegmentelevationmyocardialinfarction AT guoboxie m6aregulatormethylationpatternsandcharacteristicsofimmunityinacutestsegmentelevationmyocardialinfarction AT mingyang m6aregulatormethylationpatternsandcharacteristicsofimmunityinacutestsegmentelevationmyocardialinfarction AT guotaisheng m6aregulatormethylationpatternsandcharacteristicsofimmunityinacutestsegmentelevationmyocardialinfarction |