Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats
CONTEXT: Peripheral neuropathy is one of the chronic complications of diabetes mellitus and is directly related to gastrointestinal consequences of the disease. Myenteric neurons are affected in some pathological conditions such as diabetic neuropathy. The imbalance between cellular antioxidants and...
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| Format: | Article |
| Language: | English |
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Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia (IBEPEGE)
2011-03-01
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| Series: | Arquivos de Gastroenterologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032011000100014&lng=en&tlng=en |
| _version_ | 1819071196653682688 |
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| author | Jacqueline Nelisis Zanoni Eleandro Aparecido Tronchini Sheila Alves Moure Ivan Domicio da Silva Souza |
| author_facet | Jacqueline Nelisis Zanoni Eleandro Aparecido Tronchini Sheila Alves Moure Ivan Domicio da Silva Souza |
| author_sort | Jacqueline Nelisis Zanoni |
| collection | DOAJ |
| description | CONTEXT: Peripheral neuropathy is one of the chronic complications of diabetes mellitus and is directly related to gastrointestinal consequences of the disease. Myenteric neurons are affected in some pathological conditions such as diabetic neuropathy. The imbalance between cellular antioxidants and free radicals, leading to an increase in oxidative stress, is considered one of the main factors responsible for neuronal damages in diabetes. Drugs that reduce the oxidative stress may play a significant role in the treatment of neurological complications of diabetes mellitus. OBJECTIVE: To evaluate the effect of L-glutamine supplementation on the myenteric neurons from the cecum and duodenum of Wistar rats with streptozotocin-induced diabetes mellitus. METHODS: The animals were divided in four groups (n = 5): non-treated normoglycemics, normoglycemics treated with L-glutamine, non-treated diabetics and diabetics treated with L-glutamine from the 4th day of diabetes induction on. The amino acid L-glutamine was added to their diet at 1%. Giemsa's technique was employed to stain the myenteric neurons. We determined the cell body area of 500 neurons in each group studied. The quantitative analysis was performed by sampling in an area of 16.6 mm² in the cecum and 3.6 mm² in the duodenum of each animal. RESULTS: After the supplementation with L-glutamine in the duodenum, we observed a preservation of neuronal density in groups normoglycemic and diabetic (P<0.05). We also observed a preservation of the cell bodies area in diabetic animals (group treated with L-glutamine) (P<0.05). In the cecum, that preservation was not evident. CONCLUSION: Supplementation with L-glutamine (1%) promoted a neuroprotective effect on the myenteric neurons from the duodenum of rats, both in terms of natural aging and of diabetes mellitus. |
| first_indexed | 2024-12-21T17:17:59Z |
| format | Article |
| id | doaj.art-28b88cfedaf5484cb0215d7465d2a4cc |
| institution | Directory Open Access Journal |
| issn | 1678-4219 |
| language | English |
| last_indexed | 2024-12-21T17:17:59Z |
| publishDate | 2011-03-01 |
| publisher | Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia (IBEPEGE) |
| record_format | Article |
| series | Arquivos de Gastroenterologia |
| spelling | doaj.art-28b88cfedaf5484cb0215d7465d2a4cc2022-12-21T18:56:14ZengInstituto Brasileiro de Estudos e Pesquisas de Gastroenterologia (IBEPEGE)Arquivos de Gastroenterologia1678-42192011-03-01481667110.1590/S0004-28032011000100014S0004-28032011000100014Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic ratsJacqueline Nelisis Zanoni0Eleandro Aparecido Tronchini1Sheila Alves Moure2Ivan Domicio da Silva Souza3Universidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáCONTEXT: Peripheral neuropathy is one of the chronic complications of diabetes mellitus and is directly related to gastrointestinal consequences of the disease. Myenteric neurons are affected in some pathological conditions such as diabetic neuropathy. The imbalance between cellular antioxidants and free radicals, leading to an increase in oxidative stress, is considered one of the main factors responsible for neuronal damages in diabetes. Drugs that reduce the oxidative stress may play a significant role in the treatment of neurological complications of diabetes mellitus. OBJECTIVE: To evaluate the effect of L-glutamine supplementation on the myenteric neurons from the cecum and duodenum of Wistar rats with streptozotocin-induced diabetes mellitus. METHODS: The animals were divided in four groups (n = 5): non-treated normoglycemics, normoglycemics treated with L-glutamine, non-treated diabetics and diabetics treated with L-glutamine from the 4th day of diabetes induction on. The amino acid L-glutamine was added to their diet at 1%. Giemsa's technique was employed to stain the myenteric neurons. We determined the cell body area of 500 neurons in each group studied. The quantitative analysis was performed by sampling in an area of 16.6 mm² in the cecum and 3.6 mm² in the duodenum of each animal. RESULTS: After the supplementation with L-glutamine in the duodenum, we observed a preservation of neuronal density in groups normoglycemic and diabetic (P<0.05). We also observed a preservation of the cell bodies area in diabetic animals (group treated with L-glutamine) (P<0.05). In the cecum, that preservation was not evident. CONCLUSION: Supplementation with L-glutamine (1%) promoted a neuroprotective effect on the myenteric neurons from the duodenum of rats, both in terms of natural aging and of diabetes mellitus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032011000100014&lng=en&tlng=enGlutaminaPlexo mientéricoDiabetes mellitus, experimentalNeuropatias diabéticasEstresse oxidativoCecoDuodenoRatos |
| spellingShingle | Jacqueline Nelisis Zanoni Eleandro Aparecido Tronchini Sheila Alves Moure Ivan Domicio da Silva Souza Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats Arquivos de Gastroenterologia Glutamina Plexo mientérico Diabetes mellitus, experimental Neuropatias diabéticas Estresse oxidativo Ceco Duodeno Ratos |
| title | Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats |
| title_full | Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats |
| title_fullStr | Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats |
| title_full_unstemmed | Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats |
| title_short | Effects of L-glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats |
| title_sort | effects of l glutamine supplementation on the myenteric neurons from the duodenum and cecum of diabetic rats |
| topic | Glutamina Plexo mientérico Diabetes mellitus, experimental Neuropatias diabéticas Estresse oxidativo Ceco Duodeno Ratos |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032011000100014&lng=en&tlng=en |
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