Phenolic compounds, antioxidant and antibacterial activity of extract from leaves and bark of Stryphnodendron adstringens (Mart.) Coville
ABSTRACT There is currently growing interest in the investigation of bioactive compounds from natural sources. In this context, this study investigated the phenolic content, antioxidant capacity, and antimicrobial activity of the hydroalcoholic extract (EHA) of the stem barks and leaves of Stryphnod...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal do Ceará
2022-08-01
|
| Series: | Revista Ciência Agronômica |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902022000100448&tlng=en |
| _version_ | 1817995407934881792 |
|---|---|
| author | Jhonatas Emílio Ribeiro da Cruz João Lucas Gomes Costa Terezinha Aparecida Teixeira Guilherme Ramos Oliveira e Freitas Marcos de Souza Gomes Enyara Rezende Morais |
| author_facet | Jhonatas Emílio Ribeiro da Cruz João Lucas Gomes Costa Terezinha Aparecida Teixeira Guilherme Ramos Oliveira e Freitas Marcos de Souza Gomes Enyara Rezende Morais |
| author_sort | Jhonatas Emílio Ribeiro da Cruz |
| collection | DOAJ |
| description | ABSTRACT There is currently growing interest in the investigation of bioactive compounds from natural sources. In this context, this study investigated the phenolic content, antioxidant capacity, and antimicrobial activity of the hydroalcoholic extract (EHA) of the stem barks and leaves of Stryphnodendron adstringens. Plant parts were extracted with ethanol:water (1:1) and filtered in a vacuum to obtain the crude extract. Phenolic content was determined by the Folin-Ciocalteu method. Antioxidant activity was measured using the DPPH test. The antimicrobial activity of extracts was evaluated against Gram-positive cocci: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (strain isolated from a patient); and Gram-negative bacilli: Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853). The zone of inhibition and the minimum inhibitory concentration (MIC) of the plant extracts were evaluated by the agar diffusion disc method and broth microdilution, respectively. Total phenolic content of the EHAs was higher in stem barks (970.4 mg GAE/g) than in leaves (693.8 mg GAE/g). In the DPPH assay, all extracts showed high antioxidant activity, above 75% at the concentration of 100 μg/mL. About antimicrobial activity, all the EHAs showed effects against Gram-positive bacteria S. aureus andS. epidermidis, whose MIC ranged from 7.81 μg/mL to 62.5 μg/mL. This finding is important since these pathogens are generally resistant to a variety of widely known antibiotics. Therefore, these results are promising and indicate the use of S. adstringens (Mart.) Coville as a natural antioxidant in foods, medicines and also point to its use in antimicrobial therapy. |
| first_indexed | 2024-04-14T02:06:37Z |
| format | Article |
| id | doaj.art-28bc82274c214c58b46cc780f8d84749 |
| institution | Directory Open Access Journal |
| issn | 1806-6690 |
| language | English |
| last_indexed | 2024-04-14T02:06:37Z |
| publishDate | 2022-08-01 |
| publisher | Universidade Federal do Ceará |
| record_format | Article |
| series | Revista Ciência Agronômica |
| spelling | doaj.art-28bc82274c214c58b46cc780f8d847492022-12-22T02:18:39ZengUniversidade Federal do CearáRevista Ciência Agronômica1806-66902022-08-015310.5935/1806-6690.20220049Phenolic compounds, antioxidant and antibacterial activity of extract from leaves and bark of Stryphnodendron adstringens (Mart.) CovilleJhonatas Emílio Ribeiro da Cruzhttps://orcid.org/0000-0002-9361-6909João Lucas Gomes Costahttps://orcid.org/0000-0002-4360-1952Terezinha Aparecida Teixeirahttps://orcid.org/0000-0001-9356-7492Guilherme Ramos Oliveira e Freitashttps://orcid.org/0000-0001-6927-9730Marcos de Souza Gomeshttps://orcid.org/0000-0003-3735-2694Enyara Rezende Moraishttps://orcid.org/0000-0001-5193-889XABSTRACT There is currently growing interest in the investigation of bioactive compounds from natural sources. In this context, this study investigated the phenolic content, antioxidant capacity, and antimicrobial activity of the hydroalcoholic extract (EHA) of the stem barks and leaves of Stryphnodendron adstringens. Plant parts were extracted with ethanol:water (1:1) and filtered in a vacuum to obtain the crude extract. Phenolic content was determined by the Folin-Ciocalteu method. Antioxidant activity was measured using the DPPH test. The antimicrobial activity of extracts was evaluated against Gram-positive cocci: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (strain isolated from a patient); and Gram-negative bacilli: Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853). The zone of inhibition and the minimum inhibitory concentration (MIC) of the plant extracts were evaluated by the agar diffusion disc method and broth microdilution, respectively. Total phenolic content of the EHAs was higher in stem barks (970.4 mg GAE/g) than in leaves (693.8 mg GAE/g). In the DPPH assay, all extracts showed high antioxidant activity, above 75% at the concentration of 100 μg/mL. About antimicrobial activity, all the EHAs showed effects against Gram-positive bacteria S. aureus andS. epidermidis, whose MIC ranged from 7.81 μg/mL to 62.5 μg/mL. This finding is important since these pathogens are generally resistant to a variety of widely known antibiotics. Therefore, these results are promising and indicate the use of S. adstringens (Mart.) Coville as a natural antioxidant in foods, medicines and also point to its use in antimicrobial therapy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902022000100448&tlng=enCerradoNatural productsMinimum inhibitory concentration |
| spellingShingle | Jhonatas Emílio Ribeiro da Cruz João Lucas Gomes Costa Terezinha Aparecida Teixeira Guilherme Ramos Oliveira e Freitas Marcos de Souza Gomes Enyara Rezende Morais Phenolic compounds, antioxidant and antibacterial activity of extract from leaves and bark of Stryphnodendron adstringens (Mart.) Coville Revista Ciência Agronômica Cerrado Natural products Minimum inhibitory concentration |
| title | Phenolic compounds, antioxidant and antibacterial activity of extract from leaves and bark of Stryphnodendron adstringens (Mart.) Coville |
| title_full | Phenolic compounds, antioxidant and antibacterial activity of extract from leaves and bark of Stryphnodendron adstringens (Mart.) Coville |
| title_fullStr | Phenolic compounds, antioxidant and antibacterial activity of extract from leaves and bark of Stryphnodendron adstringens (Mart.) Coville |
| title_full_unstemmed | Phenolic compounds, antioxidant and antibacterial activity of extract from leaves and bark of Stryphnodendron adstringens (Mart.) Coville |
| title_short | Phenolic compounds, antioxidant and antibacterial activity of extract from leaves and bark of Stryphnodendron adstringens (Mart.) Coville |
| title_sort | phenolic compounds antioxidant and antibacterial activity of extract from leaves and bark of stryphnodendron adstringens mart coville |
| topic | Cerrado Natural products Minimum inhibitory concentration |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902022000100448&tlng=en |
| work_keys_str_mv | AT jhonatasemilioribeirodacruz phenoliccompoundsantioxidantandantibacterialactivityofextractfromleavesandbarkofstryphnodendronadstringensmartcoville AT joaolucasgomescosta phenoliccompoundsantioxidantandantibacterialactivityofextractfromleavesandbarkofstryphnodendronadstringensmartcoville AT terezinhaaparecidateixeira phenoliccompoundsantioxidantandantibacterialactivityofextractfromleavesandbarkofstryphnodendronadstringensmartcoville AT guilhermeramosoliveiraefreitas phenoliccompoundsantioxidantandantibacterialactivityofextractfromleavesandbarkofstryphnodendronadstringensmartcoville AT marcosdesouzagomes phenoliccompoundsantioxidantandantibacterialactivityofextractfromleavesandbarkofstryphnodendronadstringensmartcoville AT enyararezendemorais phenoliccompoundsantioxidantandantibacterialactivityofextractfromleavesandbarkofstryphnodendronadstringensmartcoville |