Significance of transcription factor LMO3 in evaluating prognosis of patients with endometrial carcinoma
Objective To investigate the significance of transcription factor LIM domain only 3 (LMO3) expression level in the prognosis of patients with endometrial carcinoma (EC). Methods The Cancer Genome Atlas (TCGA), GeneMANIA and STRING databases were used to analyze the relationship between LMO3 expressi...
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| Format: | Article |
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Editorial Office of Journal of Third Military Medical University
2022-05-01
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| Series: | Di-san junyi daxue xuebao |
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| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202110101.htm |
| _version_ | 1818237754352336896 |
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| author | LIU Jiali XUAN Beibei QUAN Quan GONG Sainan MU Xiaoling |
| author_facet | LIU Jiali XUAN Beibei QUAN Quan GONG Sainan MU Xiaoling |
| author_sort | LIU Jiali |
| collection | DOAJ |
| description | Objective To investigate the significance of transcription factor LIM domain only 3 (LMO3) expression level in the prognosis of patients with endometrial carcinoma (EC). Methods The Cancer Genome Atlas (TCGA), GeneMANIA and STRING databases were used to analyze the relationship between LMO3 expression in EC tissues and the prognosis of patients, as well as the genes interacting with LMO3, and the correlation of LMO3 with TP53 mutation. The paraffin sections and clinical information of 51 patients with EC who underwent surgery in our hospital from March 2011 to December 2014 were collected. Immunohistochemical staining was subsequently performed to determine the expression of LMO3 in EC tissues and its association with the clinicopathological features of patients. SPSS was used to plot the survival curve, and COX multivariate regression analysis was also adopted. In addition, LMO3 overexpression was conducted in EC cells to verify its effect on tumor cell migration. Results According to the analysis of TCGA database, the overall survival and disease-free survival of patients with high LMO3 expression were significantly shorter than those of low LMO3 expression(P < 0.05), and the expression level of LMO3 in patients with TP53 mutation was remarkably higher than that in TP53 wild-type patients. GeneMANIA and STRING analyses showed that the main genes interacting with LMO3 included NHLH2 and LDB2. Moreover, immunohistochemical staining also indicated that patients with higher LMO3 expression level had shorter disease-free survival (P=0.005), and multivariate COX analysis suggested LMO3 as a prognostic factor for EC. Finally, overexpression of LMO3 enhanced the migration of EC cells. Conclusion EC patients with high LMO3 level have a poor prognosis, which may be related to the migration of EC cells promoted by LMO3 overexpression. |
| first_indexed | 2024-12-12T12:30:47Z |
| format | Article |
| id | doaj.art-28c030086fa549daa0141e7a559f8fa6 |
| institution | Directory Open Access Journal |
| issn | 1000-5404 |
| language | zho |
| last_indexed | 2024-12-12T12:30:47Z |
| publishDate | 2022-05-01 |
| publisher | Editorial Office of Journal of Third Military Medical University |
| record_format | Article |
| series | Di-san junyi daxue xuebao |
| spelling | doaj.art-28c030086fa549daa0141e7a559f8fa62022-12-22T00:24:25ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042022-05-0144101054106010.16016/j.2097-0927.202110101Significance of transcription factor LMO3 in evaluating prognosis of patients with endometrial carcinomaLIU Jiali0XUAN Beibei1QUAN Quan2GONG Sainan3MU Xiaoling4Department of Obstetrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, ChinaDepartment of Obstetrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, ChinaDepartment of Obstetrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, ChinaDepartment of Obstetrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, ChinaDepartment of Obstetrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, ChinaObjective To investigate the significance of transcription factor LIM domain only 3 (LMO3) expression level in the prognosis of patients with endometrial carcinoma (EC). Methods The Cancer Genome Atlas (TCGA), GeneMANIA and STRING databases were used to analyze the relationship between LMO3 expression in EC tissues and the prognosis of patients, as well as the genes interacting with LMO3, and the correlation of LMO3 with TP53 mutation. The paraffin sections and clinical information of 51 patients with EC who underwent surgery in our hospital from March 2011 to December 2014 were collected. Immunohistochemical staining was subsequently performed to determine the expression of LMO3 in EC tissues and its association with the clinicopathological features of patients. SPSS was used to plot the survival curve, and COX multivariate regression analysis was also adopted. In addition, LMO3 overexpression was conducted in EC cells to verify its effect on tumor cell migration. Results According to the analysis of TCGA database, the overall survival and disease-free survival of patients with high LMO3 expression were significantly shorter than those of low LMO3 expression(P < 0.05), and the expression level of LMO3 in patients with TP53 mutation was remarkably higher than that in TP53 wild-type patients. GeneMANIA and STRING analyses showed that the main genes interacting with LMO3 included NHLH2 and LDB2. Moreover, immunohistochemical staining also indicated that patients with higher LMO3 expression level had shorter disease-free survival (P=0.005), and multivariate COX analysis suggested LMO3 as a prognostic factor for EC. Finally, overexpression of LMO3 enhanced the migration of EC cells. Conclusion EC patients with high LMO3 level have a poor prognosis, which may be related to the migration of EC cells promoted by LMO3 overexpression.http://aammt.tmmu.edu.cn/Upload/rhtml/202110101.htmtranscription factorlim domain only 3endometrial carcinomaprognosisbioinformatics |
| spellingShingle | LIU Jiali XUAN Beibei QUAN Quan GONG Sainan MU Xiaoling Significance of transcription factor LMO3 in evaluating prognosis of patients with endometrial carcinoma Di-san junyi daxue xuebao transcription factor lim domain only 3 endometrial carcinoma prognosis bioinformatics |
| title | Significance of transcription factor LMO3 in evaluating prognosis of patients with endometrial carcinoma |
| title_full | Significance of transcription factor LMO3 in evaluating prognosis of patients with endometrial carcinoma |
| title_fullStr | Significance of transcription factor LMO3 in evaluating prognosis of patients with endometrial carcinoma |
| title_full_unstemmed | Significance of transcription factor LMO3 in evaluating prognosis of patients with endometrial carcinoma |
| title_short | Significance of transcription factor LMO3 in evaluating prognosis of patients with endometrial carcinoma |
| title_sort | significance of transcription factor lmo3 in evaluating prognosis of patients with endometrial carcinoma |
| topic | transcription factor lim domain only 3 endometrial carcinoma prognosis bioinformatics |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202110101.htm |
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