Detection of OXA-Type Carbapenemase Genes in Acinetobacter baumannii Isolates from Nosocomial Infections in Isfahan Hospitals, Iran
"> <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><strong><em>Background: </em></strong><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>Acine...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2016-02-01
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| Series: | Journal of Medical Bacteriology |
| Subjects: | |
| Online Access: | http://jmb.tums.ac.ir/index.php/jmb/article/view/110 |
| Summary: | "> <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><strong><em>Background: </em></strong><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>Acinetobacter baumannii </em><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">as one of the causes of nosocomial infections has become<br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">resistant to almost all antimicrobial agents. The emergence of resistance to carbapenems, one of<br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">the last drugs on the shelf, is the major concern about <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>A. baumannii </em><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">antimicrobial resistance.<br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">Resistance to carbapenems is mediated by production of class B and D carbapenemases. The aim<br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">of this study was to detect the resistance genes including <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>bla</em><span style="font-size: 6pt; color: #000000; font-style: normal; font-variant: normal;">OXA-23, 24, 51, <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">and <span style="font-size: 6pt; color: #000000; font-style: normal; font-variant: normal;">58 <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">in <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>A. baumannii</em><br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">isolates from nosocomial infections in Isfahan hospitals.<br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><strong><em>Methods</em></strong><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><strong>: </strong><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">A total number of 456 clinical specimens were collected from nosocomial infections and<br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">evaluated in order to isolate <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>A. baumannii </em><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">strains. After identification of the isolates, the antibiotic<br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">sensitivity to carbapenems was assessed using disk diffusion method. The resistance genes of <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>bla</em><br /><span style="font-size: 6pt; color: #000000; font-style: normal; font-variant: normal;">OXA-23, 24, 51, <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">and <span style="font-size: 6pt; color: #000000; font-style: normal; font-variant: normal;">58 <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">were detected by multiplex PCR method.<br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><strong><em>Results: </em></strong><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">Fifty <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>A. baumannii </em><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">isolates were isolated from clinical specimens. Fifty two percent of<br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">the isolates showed phenotypic resistance to the carbapenems (imipenem and meropenem).<br /><span style="font-family: Times New Roman; font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">According to PCR results, 88% of resistant isolates had ≥1 <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>bla</em><span style="font-size: 6pt; color: #000000; font-style: normal; font-variant: normal;">OXA <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">gene. The frequency of resistant<br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">isolates bearing <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>bla</em><span style="font-size: 6pt; color: #000000; font-style: normal; font-variant: normal;">OXA-23<span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">, <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>bla</em><span style="font-size: 6pt; color: #000000; font-style: normal; font-variant: normal;">OXA-24 <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">and <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>bla</em><span style="font-size: 6pt; color: #000000; font-style: normal; font-variant: normal;">OXA-58 <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">were 77%, 38% and 15% respectively.<br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><strong><em>Conclusions</em></strong><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">: This study showed the high frequency of carbapenem resistance genes among <span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>A.</em><br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;"><em>baumannii </em><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">isolates. Therefore, adopting an appropriate strategy to confine the spreading of these<br /><span style="font-size: 9pt; color: #000000; font-style: normal; font-variant: normal;">strains and also implementing new treatment regimens are necessary.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span> |
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| ISSN: | 2251-8649 2322-2581 |